Difference between revisions of "Team:SCU-WestChina/Demonstrate"

 
(8 intermediate revisions by one other user not shown)
Line 1: Line 1:
−
{{SCU-WestChina}}
+
{{:Team:SCU-WestChina/default.css}}
 +
{{:Team:SCU-WestChina/style.css}}
  
 
<html>
 
<html>
  
 +
<head>
 +
    <meta charset="utf-8">
 +
    <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css">
 +
    <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
 +
   
 +
    <title>index</title>
 +
</head>
  
 +
<body>
 +
    <ul class="nav nav-pills">
 +
        <img src="https://static.igem.org/mediawiki/2017/b/b9/T--SCU-WestChina--logo.png" alt="" class="logo">
 +
        <div class="nav-right-wrapper">
 +
            <li role="presentation">
 +
                <a href="https://2017.igem.org/Team:SCU-WestChina">Home</a>
 +
            </li>
 +
            <li role="presentation">
 +
                <a href="https://2017.igem.org/Team:SCU-WestChina/Background">Background</a>
 +
            </li>
 +
            <li role="presentation" class="dropdown">
 +
                <a class="dropdown-toggle" data-toggle="dropdown" href="#" role="button">
 +
                    Project
 +
                </a>
 +
                <ul class="dropdown-menu">
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/Description">Description</a>
 +
                    </li>
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/Demonstrate">Proof</a>
 +
                    </li>
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/Parts">Parts</a>
 +
                    </li>
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/Measurement">Measurement</a>
 +
                    </li>
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/Hardware">Hardware</a>
 +
                    </li>
 +
                </ul>
 +
            </li>
 +
            <li role="presentation">
 +
                <a href="https://2017.igem.org/Team:SCU-WestChina/Model">Modeling</a>
 +
            </li>
 +
            <li role="presentation" class="dropdown">
 +
                <a class="dropdown-toggle" data-toggle="dropdown" href="#" role="button">
 +
                    Human Practice
 +
                </a>
 +
                <ul class="dropdown-menu">
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/HP/Silver">Silver</a>
 +
                    </li>
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/HP/Gold_Integrated">Gold</a>
 +
                    </li>
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/Engagement">Education and Public Engagement</a>
 +
                    </li>
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/Collaborations">Collaborations</a>
 +
                    </li>
 +
                </ul>
 +
            </li>
 +
            <li role="presentation" class="dropdown">
 +
                <a class="dropdown-toggle" data-toggle="dropdown" href="#" role="button">
 +
                    Notebook
 +
                </a>
 +
                <ul class="dropdown-menu">
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/Notebook">Notebook</a>
 +
                    </li>
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/Protocols">Protocols</a>
 +
                    </li>
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/Medals">Medals</a>
 +
                    </li>
 +
                </ul>
 +
            </li>
 +
            <li role="presentation" class="dropdown">
 +
                <a class="dropdown-toggle" data-toggle="dropdown" href="#" role="button">
 +
                    Team
 +
                </a>
 +
                <ul class="dropdown-menu">
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/Team">Team</a>
 +
                    </li>
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/Attributions">Attributions</a>
 +
                    </li>
 +
                    <li>
 +
                        <a href="https://2017.igem.org/Team:SCU-WestChina/Acknowledgement">Acknowledgement</a>
 +
                    </li>
 +
                </ul>
 +
            </li>
 +
            <li role="presentation">
 +
                <a href="https://2017.igem.org/Team:SCU-WestChina/InterLab">InterLab</a>
 +
            </li>
 +
        </div>
 +
    </ul>
 +
    <div class="main-wrapper">
 +
        <div class="primary-wrapper">
 +
            <div class="primary-title">Proof</div>
 +
            <div class="primary-content"><br>The real condition in the human gut is so complicated: a. Nutrition environment is hard to simulate because of the circadian rhythm of food intake, the food eaten and the enzymes released by intestine. b. The effect of gut microbiome. c. the peristalsis process in the intestine. d. the oxygen environment. To evaluate whether our project can work in the real condition, we decided to do the animal experiment.</br>
 +
            </div>
 +
        </div>
 +
        <div class="mid-wrapper">
  
−
<div class="column full_size">
+
<div class="title-center">Reducing the urate concentration in the gut for HUA patients.</div>
−
<h1>Demonstrate</h1>
+
<p>To prove the function of the bacteria in the real condition, we conducted the animal experiment.</p>
−
<h3>Gold Medal Criterion #4</h3>
+
<p>We tried to establish the HUA mouse model in the first stage in our animal experiment. We divided 10 male 5-week-aged balb/c mice into two groups randomly: Group 1 (n=5) and Group 2 (n=5). Set one week’s time without special handling to let them adapt to the new environment. Feed Group 2 with special food containing 0.1% adenine since week 2 while feeding Group 1 with normal food. All the feeding condition is the same expect for food.The blood of all the mice from caudal vein was taken after 3 weeks’ feeding. Isolate the serum and demonstrate the concentration of blood uric acid with ELISA.
 +
The result showed that, we can use the method to establish the HUA model successfully (Figure 11a.).</p>
  
−
<p>
+
<p>Then, the engineered bacteria was used in the animal experiment. We divided 25 male 5-week-aged balb/c mice into five groups randomly: Group Control (Group A, n=5), Group HUA (Group B, n=5), Group Allopurinol (Group C, n=5), Group engineered <i>E. coli</i> Nissle 1917 (transformed with BBa_K2334001) (Group D,n=5) and Group <i>E. coli</i> Nissle 1917 (Group E, n=5) [Figure 11b]. Set one week’s time without special handling to let them adapt to the new environment. Establish hyperuricemia animal models with all the groups except Group Control.Performe oval gavage as protocol from week 2, last for 3 weeks Isolate the serum and demonstrate the concentration of blood uric acid with ELISA.</p>  
−
Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
+
−
</p>
+
  
−
<p>
+
<p>Allopurinol is used in clinics to reduce the serum urate concentration, whose group is the negative control. Group B and C indicated that we established the HUA model successfully. Group D is the experiment group, which is of distinctive lower urate concentration compared to Group A. However, the group E has similar performance as Group D, which suggested <i>E. coli</i> Nissle 1917 itself has a ability to reduce the serum urate concentration, which is so surprising. We can't determine if our engineered pathway is working in vivo. But it's clear our engineered bacteria itself works. It may be related to the remodeling of the gut microbiome if a lot of bacteria were given by oral gavage (Figure 11c). Though no group diarrhea was found.</p>
−
Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
+
−
</p>
+
  
 +
            <img src="https://static.igem.org/mediawiki/2017/3/33/Scuwestchina-am.jpg" alt="">
 +
            <div class="img-describe">
 +
              Figure 11 (a) The urate concentration in serum in experiment group is higher than that in control group obviously, which indicated the successful establishment of HUA mouse model. (b) The arrangement of engineered bacteria in vivo experiment. (c) Group Control (Group A, n=5), Group HUA (Group B, n=5), Group Allopurinol (Group C, n=5), Group engineered <i>E. coli</i> Nissle 1917 (Nissle 1917 transformed with BBa_K2334001, Group D,n=5) and Group <i>E. coli</i> Nissle 1917 (Group E, n=5). The urate concentration in serum in engineerd bacteria experiment. Group B and C indicated we established the HUA model successfully. The experiment group has obviously lower urate concentration than that of the control group A. However, there's no significant difference between group D and E.
 +
            </div>
  
−
</div>
 
  
 +
    </div>
 +
    <script src="https://code.jquery.com/jquery-3.2.1.min.js"></script>
 +
    <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
 +
    <!-- <script src="https://cdn.bootcss.com/echarts/3.7.2/echarts.min.js"></script> -->
 +
</body>
  
−
<div class="column half_size">
 
  
−
<h4> What should we do for our demonstration?</h4>
 
−
 
−
<h5> Standard teams </h5>
 
−
 
−
<p>
 
−
If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
 
</p>
 
</p>
−
</div>
+
<!--
 +
NewPP limit report
 +
CPU time usage: 0.007 seconds
 +
Real time usage: 0.009 seconds
 +
Preprocessor visited node count: 14/1000000
 +
Preprocessor generated node count: 64/1000000
 +
Post‐expand include size: 82/2097152 bytes
 +
Template argument size: 0/2097152 bytes
 +
Highest expansion depth: 2/40
 +
Expensive parser function count: 0/100
 +
-->
  
−
<div class="column half_size">
+
<!-- Saved in parser cache with key 2017_igem_org:pcache:idhash:53983-0!*!*!*!*!*!* and timestamp 20171030155604 and revision id 339167
 +
-->
 +
</div>                  <div class="visualClear"></div>
 +
                </div>
 +
            </div>
  
−
<br>
+
        </div>
−
<h5> Special track teams </h5>
+
  
−
<p>
+
        <!-- Side Menubar -->
−
Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
+
        <div id="sideMenu">
−
</p>
+
            <a href="https://2017.igem.org">
 +
                <div id="home_logo" >
 +
                <img src="https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg">
 +
                </div>
 +
            </a>
  
 +
            <div style="clear:both; height:5px;"></div>
  
−
</div>
+
            <div id="menuDisplay"></div>  <!- Menu will be loaded here ->
 +
        </div>
  
 +
        <!-- Pop_Why Div is definded here -->
 +
        <div class="pop_why_cover"></div>
  
 +
        <div class="pop_why_box" >
 +
            <div class="pop_close">× </div>
 +
            <div class="pop_why_content"><h3> Loading ... </h3></div>
 +
        </div>
  
 +
    </div>
 
</html>
 
</html>

Latest revision as of 17:22, 1 November 2017

index

Proof

The real condition in the human gut is so complicated: a. Nutrition environment is hard to simulate because of the circadian rhythm of food intake, the food eaten and the enzymes released by intestine. b. The effect of gut microbiome. c. the peristalsis process in the intestine. d. the oxygen environment. To evaluate whether our project can work in the real condition, we decided to do the animal experiment.
Reducing the urate concentration in the gut for HUA patients.

To prove the function of the bacteria in the real condition, we conducted the animal experiment.

We tried to establish the HUA mouse model in the first stage in our animal experiment. We divided 10 male 5-week-aged balb/c mice into two groups randomly: Group 1 (n=5) and Group 2 (n=5). Set one week’s time without special handling to let them adapt to the new environment. Feed Group 2 with special food containing 0.1% adenine since week 2 while feeding Group 1 with normal food. All the feeding condition is the same expect for food.The blood of all the mice from caudal vein was taken after 3 weeks’ feeding. Isolate the serum and demonstrate the concentration of blood uric acid with ELISA. The result showed that, we can use the method to establish the HUA model successfully (Figure 11a.).

Then, the engineered bacteria was used in the animal experiment. We divided 25 male 5-week-aged balb/c mice into five groups randomly: Group Control (Group A, n=5), Group HUA (Group B, n=5), Group Allopurinol (Group C, n=5), Group engineered E. coli Nissle 1917 (transformed with BBa_K2334001) (Group D,n=5) and Group E. coli Nissle 1917 (Group E, n=5) [Figure 11b]. Set one week’s time without special handling to let them adapt to the new environment. Establish hyperuricemia animal models with all the groups except Group Control.Performe oval gavage as protocol from week 2, last for 3 weeks Isolate the serum and demonstrate the concentration of blood uric acid with ELISA.

Allopurinol is used in clinics to reduce the serum urate concentration, whose group is the negative control. Group B and C indicated that we established the HUA model successfully. Group D is the experiment group, which is of distinctive lower urate concentration compared to Group A. However, the group E has similar performance as Group D, which suggested E. coli Nissle 1917 itself has a ability to reduce the serum urate concentration, which is so surprising. We can't determine if our engineered pathway is working in vivo. But it's clear our engineered bacteria itself works. It may be related to the remodeling of the gut microbiome if a lot of bacteria were given by oral gavage (Figure 11c). Though no group diarrhea was found.

Figure 11 (a) The urate concentration in serum in experiment group is higher than that in control group obviously, which indicated the successful establishment of HUA mouse model. (b) The arrangement of engineered bacteria in vivo experiment. (c) Group Control (Group A, n=5), Group HUA (Group B, n=5), Group Allopurinol (Group C, n=5), Group engineered E. coli Nissle 1917 (Nissle 1917 transformed with BBa_K2334001, Group D,n=5) and Group E. coli Nissle 1917 (Group E, n=5). The urate concentration in serum in engineerd bacteria experiment. Group B and C indicated we established the HUA model successfully. The experiment group has obviously lower urate concentration than that of the control group A. However, there's no significant difference between group D and E.

×

Loading ...