Difference between revisions of "Team:HokkaidoU Japan"

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<h1>HokkaidoU_Japan Project: E.co Circle</h1>
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<h1> Project discription </h1>
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<p> Eutrophication is a phenomenon that undermines our lives quietly but steadily by polluting water or damaging fisheries. Our team aim to solve the problem through stock-breeding by utilizing self-assembling peptides (SAPs), the materials now getting more and more attention in many fields. Using SAPs, we try to reduce the excess leakage of nutrition from the farm. </p>
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<h2>Overview</h2>
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<p>Today, we have a problem over stock breeding that excrement of livestock causes eutrophication of rivers. This is because they contain phytic acid which has 6 phosphate groups. Moreover, phytic acid chelate and prevent livestock from absorbing minerals. Nowadays, phytase, an enzyme that degrades phytic acids, is widely added to the feed to increase mineral absorption and to alleviate eutrophication. However, feed is sterilized with heat during its production and the activity of phytase drops. To solve this problem, we have come up with a way to maintain the phytase activity by connecting a linker containing Self Assembling Peptides (SAP) to its end. By doing so, we aim to form disulfide bonds between linkers to make phytase circularized and stabilized. </p>
  
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<p> This year, we work on following projects: phytase strengthening, fertilizer production and phosphate retrieve. Phytase is an enzyme that stocks take in with food and it helps them absorb nutrition by degrading phytic acid which contains 6 phosphate and chelate minerals. We try to circularize the enzyme using SAPs to increase the enzyme stability against heat or extreme pH. It may lead enhancing absorption of nutrition and decrease the amount of phosphate in the excretion.
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<img src="https://static.igem.org/mediawiki/2017/thumb/d/d6/T--HokkaidoU_Japan--overviewushi.png/788px-T--HokkaidoU_Japan--overviewushi.png" width="700px"><p><br></p>
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Production of fertilizer from the excrement is now widely performed under the status quo, however, since the feces often contains urine, it holds too much water to advance fermentation and it requires time and labor to make proper fertilizer. We try to let the bacteria in the intestine of animals produce gels made of SAPs that holds appropriate amount of water and realize the fertilizer production without trouble. While 2nd project is mainly focusing on feces, we also planning to use urine to retrieve phosphate. By producing gels of SAPs that chelate phosphate, we expect to reduce the leakage of phosphate and enable to reuse them. </p>
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<h2>ACHIEVEMENT</h2>
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<p>We met all the criteria shown below!<br></p>
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<h3 class="bronzestatue">BRONZE</h3>
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    <li>We registered our team, had a fantastic summer, and we are going to Giant Jamboree.</li>
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<h1> Welcome to iGEM 2017! </h1>
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    <li>We met all the Competition deriberables.</li>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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    <li>We created our Wiki with attribution.</li>
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    <li>We characterized existing BioBrick parts, <a href="http://parts.igem.org/Part:BBa_K912000">BBa_K912000</a> and <a href="http://parts.igem.org/Part:BBa_K912001">BBa_K912001</a>.</li>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2017.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2017.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2017.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2017.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2017.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2017.igem.org/Calendar">iGEM 2017 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2017 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)<br><br>
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UPLOAD FILES
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<h3 class="silverback">SILVER</h3>
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    <li>We assayed two new BioBrick parts, <a href="http://parts.igem.org/Part:BBa_K2414002">BBa_K2414002</a> and <a href="http://parts.igem.org/Part:BBa_K2414003">BBa_K2414003</a>. Also see <a href="/Team:HokkaidoU_Japan/Results">Results</a>.</li>
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    <li>We collaborated with other iGEM teams. See<a href="https://2017.igem.org/Team:HokkaidoU_Japan/Collaborations"> Collaborations</a> page.</li>
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    <li>We conducted well-thought Human Practices. See <a href="https://2017.igem.org/Team:HokkaidoU_Japan/HP/Silver">Silve_HP</a> page.</li>
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<a href="https://igem.org/2017_Judging_Form?team=HokkaidoU_Japan"> Judging form</a>
  
  
  
 
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Latest revision as of 03:59, 2 November 2017

HokkaidoU_Japan Project: E.co Circle


Overview

Today, we have a problem over stock breeding that excrement of livestock causes eutrophication of rivers. This is because they contain phytic acid which has 6 phosphate groups. Moreover, phytic acid chelate and prevent livestock from absorbing minerals. Nowadays, phytase, an enzyme that degrades phytic acids, is widely added to the feed to increase mineral absorption and to alleviate eutrophication. However, feed is sterilized with heat during its production and the activity of phytase drops. To solve this problem, we have come up with a way to maintain the phytase activity by connecting a linker containing Self Assembling Peptides (SAP) to its end. By doing so, we aim to form disulfide bonds between linkers to make phytase circularized and stabilized.


ACHIEVEMENT

We met all the criteria shown below!

BRONZE

  1. We registered our team, had a fantastic summer, and we are going to Giant Jamboree.
  2. We met all the Competition deriberables.
  3. We created our Wiki with attribution.
  4. We characterized existing BioBrick parts, BBa_K912000 and BBa_K912001.

SILVER

  1. We assayed two new BioBrick parts, BBa_K2414002 and BBa_K2414003. Also see Results.
  2. We collaborated with other iGEM teams. See Collaborations page.
  3. We conducted well-thought Human Practices. See Silve_HP page.


Judging form