Line 163: | Line 163: | ||
<div id="protocol_name"> | <div id="protocol_name"> | ||
− | <a href="#protocol3" style="text-decoration: none;"><h2 class="titre_protocole"> | + | <a href="#protocol3" style="text-decoration: none;"><h2 class="titre_protocole"> Electrophorese </h2></a> |
</div> | </div> | ||
<div id="protocol3"> | <div id="protocol3"> | ||
<div class="fleche_bas"></div> | <div class="fleche_bas"></div> | ||
− | <h5 style="padding-top:5%;"> | + | <h5 style="padding-top:5%;"> |
+ | Materials : | ||
+ | <li>1g Agarose (powder)</li> | ||
+ | <li>100mL TAE</li> | ||
+ | <li>Distillated Water</li> | ||
+ | <li>25uL Gel Red</li> | ||
+ | |||
+ | Methods : | ||
+ | <ol> | ||
+ | <li>Gel preparation</li> | ||
+ | <ol> | ||
+ | <li>Dissolve 1g of agarose in 100mL of TAE (1X)</li> | ||
+ | <li>Pour the solution into the gel mould</li> | ||
+ | <li>Let the solution gel (almost 15 minutes)</li> | ||
+ | </ol> | ||
+ | <li>Preparation of the samples to deposit</li> | ||
+ | <li>The migration is done at 100V during 30 minutes</li> | ||
+ | <li>Revelation</li> | ||
+ | <ol> | ||
+ | <li>Prepare 250mL of distillated water in a tank</li> | ||
+ | <li>Add 25uL of Gel Red. Do not forget to use gloves to manipulate the Gel Red.</li> | ||
+ | <li>Incubate the gel in the solution for 10 minutes at the obscurity</li> | ||
+ | <li>Wash the gel in a tank of distillated water</li> | ||
+ | </ol> | ||
+ | </ol> | ||
+ | </h5> | ||
<a href="#cat1" style="text-decoration: none;"><h2 class="top">TOP</h2></a> | <a href="#cat1" style="text-decoration: none;"><h2 class="top">TOP</h2></a> | ||
<div class="fleche_haut"></div> | <div class="fleche_haut"></div> |
Revision as of 15:46, 28 August 2017
Protocols
Categorie 1
Materials :
- Add 20g of LB Broth Base per 1L of distillated water
- Homogenize
- Autoclave at 121°C for 15 minutes
TOP
Materials :
- Add 32g of LB Agar per 1L of distillated water
- Homogenize
- Autoclave at 121°C for 15 minutes
TOP
Materials :
- Gel preparation
- Dissolve 1g of agarose in 100mL of TAE (1X)
- Pour the solution into the gel mould
- Let the solution gel (almost 15 minutes)
- Preparation of the samples to deposit
- The migration is done at 100V during 30 minutes
- Revelation
- Prepare 250mL of distillated water in a tank
- Add 25uL of Gel Red. Do not forget to use gloves to manipulate the Gel Red.
- Incubate the gel in the solution for 10 minutes at the obscurity
- Wash the gel in a tank of distillated water
TOP
Categorie 2
blablabla
TOP
blablabla
TOP
blablabla
TOP
Categorie 3
blablabla
TOP
blablabla
TOP
blablabla
TOP