Difference between revisions of "Team:Grenoble-Alpes/Protocols"
| Line 125: | Line 125: | ||
</center></div> | </center></div> | ||
| − | <!- -------------- PROTOCOLS | + | <!- -------------- PROTOCOLS DETAILS --------------- -> |
<div id="lb_broth"> | <div id="lb_broth"> | ||
Revision as of 15:30, 29 August 2017
Protocols
Preparations
Experimentations
LB Broth
Materials
Materials
- Add 20g of LB Broth Base per 1L of distillated water
- Homogenize
- Autoclave at 121°C for 15 minutes
TOP
LB Agar
Materials
Materials
- Add 32g of LB Agar per 1L of distilled water
- Homogenize
- Autoclave at 121°C for 15 minutes
TOP
Chloramphenicol (Cam) 25ug/mL Stock
Materials
Materials
- Add 0,25g of Cam per 10mL of ethanol
- Homogenize by vortexing
- Filter with a 22mm membrane filter
- Conserve at -20°C in 1mL aliquot
TOP
Petri Dish Preparation
Materials
Materials
- Add of 20uL Cam in 20mL of liquid LB Agar
- Pour the solution in the plate
- Wait until the agar solidifies
- Conserve returned in 4°C
TOP
Glycerol 80%
Materials
Materials
- Harvest 80mL of glycerol 100%
- Add 20mL of sterile water
- Conserve at 4°C
TOP
CaCl2 100mM
Materials
Materials
- Dissolve 0,056g of CaCl2 in 5mL of sterile water
- Conserve at 4°C
TOP
MgCl2 100mM
Materials
Materials
- Dissolve 0,6105g of MgCl2 in 30mL of sterile water
- Conserve at 4°C
TOP
Sucrose 100mM
Materials
Materials
- Dissolve 0,171g of sucrose in 5mL of sterile water
- Conserve at 4°C
TOP
IPTG 4mM
Materials
Materials
- Add 10uL of Cam in 10mL of LB
- Homogenize
- Remove 90uL of solution and add 80uL of IPTG
- Conserve at -20°C
TOP
