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Text here about the PCR reactions to create the fragments for Gibson Assembly | Text here about the PCR reactions to create the fragments for Gibson Assembly | ||
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Revision as of 02:45, 1 November 2017
Replace this with text describing the construction of our BioBricks (3.1 and 3.2) - in other words, the colony PCR and sequencing Data
PCR to Generate Fragments for Gibson Assembly
Text here about the PCR reactions to create the fragments for Gibson Assembly
Colony PCR to Verify Clone is Correct
Text here about the PCR reactions to create the fragments for Gibson Assembly
Sequencing Verification
Text here about the PCR reactions to create the fragments for Gibson Assembly
Example sequencing results for construct K2268004
> VF2 Primer result, reverse complement GGGNACGTTACGTTGCGCATTTAACTATTAGATGCGTCGAATCACGAGGCAGGAATTTCAGATAAAAAAAATCCTTAGCT TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTAACACCGTGCGTGTTGACTATTTTACCTCTGGCGGTG ATAATGGTTGCGCTAGCAAAGAGGAGAAAGCTTATGACCATGATTACGGATTCACTGGCCGTCGTTTTACAACTCGTGAC TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGC CCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAAT
> 3.2_LinkR Primer result, reverse complement.
ATCACCAGCTCCCGTCTTTCATTGCATACGAAATTCCGGAGAGCATTCATCAGGCGGGCA
AGAAATGTGAATAAAGGCCGAATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAA
GGCCGTAATATCCAACTGAACGGTCTGGTTATAGGTACATTGAGCACTGACTGAAATGCC
CTCAAAATGTTCTTTACGATGCCATTGGGATATATCACCGGTGGTATATCCAGTGATTTT
TTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGG
TAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAG
TGCCACCTGACGTCTAAGAACCCATTATTATCGTGACATTAACCTATAAAAATAGGCGTA
TCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGA
ATTCGCGGCCGCTTCTAGAGTAACACCGTGCGTGTTGACTATTTTACCTCTGGCGGTGAT
AATGGTTGCGCTAGCAAAGAGGAGAAAGCTTATGACCATGATTACGGATTCACTGGCCGT
CGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGC
ACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCA
ACAGAGGGCAGCTTGAATGGCGAATGGGCTTTGCCTGGTTTTTCGAATTCTTCCGCAACT
TACTGCGTAGCGNA
> 3.2_LinkF Primer result NTCCATGATAAGTACTCGCACTTTCTCAGCAGTCATCAGTTCGCCGCGCCAAACGTCTCTTCAGGCCACTGACTAGCGAT AACTTTCCCCACAACGGAACAACTCTCATTGCATGGGATCATTGGGTACTGTGGGTTTAGTGGTTGTAAAAACACCTGAC CGCTATCCCTGATCAGTTTCTTGAAGGTAAACTCATCACCCCCAAGTCTGGCTATGCAGAAATCACCTGGCTCAACAGCC TGCTCAGGGTCAACGAGAATTAACATTCCGTCAGGAAAGCTTGGCTTGGAGCCTGTTGGTGCGGTCATGGAATTACCTTC AACCTCAAGCCAGAATGCAGAATCACTGGCTTTTTTGGTTGTGCTTACCCATCTCTCCGCATCACCTTTGGTAAAGGTTC TAAGCTAAGGTGAGAACATCCCTGCCTGAACATGAGAAAAAACAGGGTACTCATACTCACTTCTAAGTGACGGCTGCATA CTAACCGCTTCATACATCTCGTAGATTTCTCTGGCGATTGAAGGGCTAAATTCTTCAACGCTAACTTTGAGAATTTTTGC AAGCAATGCGGCGTTATAAGCATTTAATGCATTGATGCCATTAAATAAAGCACCAACGCCTGACTGCCCCATCCCCATCT TGTCTGCGACAGATTCCTGGGATAAGCCAAGTTCATTTTTCTTTTTTTCATAAATTGCTTTAAGGCGACGTGCGTCCTCA AGCTGCTCTTGTGTTAATGGTTTCTTTTTTGTGCTCATAAGCTTTCTTCCTCTTTGCTAGCTGTGCTCAGTATCTTGTTA TCCGCTCACAATTTACTAGTAGCGGCCGCTGCAGGCCCGGCAAAAAAGGCAAGGGGTCACCACCCTGCCCTTTTTCTTTA AAACCGAAAAGATTACTTCGCGTTATGCAGGGTTCCTCGCTCACTGACTCGCTGCGCTCGGGCGTCGGCTGCGGCGGAGC GGATCAGCTCACTCAAGCGGTAATACGGTATCACAGAATCAGGGATACCGCAGAAGAACATGTGAGCAAAGGCAGCAAAG GCAGGACTCGTAAAAGCCGCGTGCTGGCGATTCACAAGGCTCGCCCCCTGACGGCACTACAAAATCGACGCTCTAGGCAG AGGTGCGAACCGCACGGACATAAAGGATCCAGC
> VR Primer result, reverse complement
ATGATTCGGATCCCTGGCCGTGTTTACACGTTCGGAACTGGGAAACCCTGCGTACCAACT
AATCCCTTGCAGCACATCCCTTTCGCAGCTGCGTAATAGCGAAGAGGCCGCCCGATCGCC
CTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGCGCTTGCCTGGTTTCCGGCACCAGAA
GCGGTGCCGAAAGCTGGCTGGAGTAATATTATTACGCCACCAGCGCATAGTTTTCATCGT
TCGCGCGCAAACGTCTCTTCAGGCACTGACTAGCGATAACTTTCCCCACAACGGAACACC
TCTCATTGCATGGGATCATTGGGTACTGTGGGTTTAGTGGTTGTAAAAACACCTGACCGC
TATCCCTGATCAGTTTCTTGAAGGTAAACTCATCACCCCCAAGTCTGGCTATGCAGAAAT
CACCTGGCTCAACAGCCTGCTCAGGGTCAACGAGAATTAACATTCCGTCAGGAAAGCTTG
GCTTGGAGCCTGTTGGTGCGGTCATGGAATTACCTTCAACCTCAAGCCAGAATGCAGAAT
CACTGGCTTTTTTGGTTGTGCTTACCCATCTCTCCGCATCACCTTTGGTAAAGGTTCTAA
GCTAAGGTGAGAACATCCCTGCCTGAACATGAGAAAAAACAGGGTACTCATACTCACTTC
TAAGTGACGGCTGCATACTAACCGCTTCATACATCTCGTAGATTTCTCTGGCGATTGAAG
GGCTAAATTCTTCAACGCTAACTTTGAGAATTTTTGCAAGCAATGCGGCGTTATAAGCAT
TTAATGCATTGATGCCATTAAATAAAGCACCAACGCCTGACTGCCCCATCCCCATCTTGT
CTGCGACAGATTCCTGGGATAAGCCAAGTTCATTTTTCTTTTTTTCATAAATTGCTTTAA
GGCGACGTGCGTCCTCAAGCTGCTCTTGTGTTAATGGTTTCTTTTTTGTGCTCATAAGCG
TTAGTATAACATTGAAGAGCTATTCGCATCACACGGATCTCCGAGCGCATTGAGCCATTG
AACGAGGAATCGGAAGAGCGCCCATTAATCAATCGCCCCTCGCGCGGGGAGAGGCGGTTT
GCGTATTGGGCGCTCTGCCCCTCACTCGCTCCCTCTGGAAACTGCGGCAGTACGTTGCGC
GGAATGTAAGG