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<p style="font-size:1.6em;color:black;margin-bottom:4px"><b>Production Plasmid</b></p> | <p style="font-size:1.6em;color:black;margin-bottom:4px"><b>Production Plasmid</b></p> | ||
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Revision as of 03:18, 2 November 2017
Design
Production Plasmid
Part of our project revolved around an assembly that produced L-DOPA (levadopa or L-3,4-dihydroxyphenylalanine) from glucose.
We constructed our production assembly by cloning a CP25/PA3-hpaBC-rpoc transcriptional unit into a vector containing a --- ori and spectinomycin resistance marker. Our construct was assembled using golden gate cloning techniques.
Diagram here.
Here is a brief overview of each main constituent of the hpaBC transcriptional unit:
Sensing Plasmid
The second half of our project revolved around an assembly that sensed the production of L-DOPA.
Our sensing construct consisted of several ---. We constructed this assembly using golden gate, gibson, and traditional cloning techniques.
Diagram here.
Here is a brief overview of the transcriptional units and their functions.
lacI Transcriptional Unit -
This page is different to the "Applied Design Award" page. Please see the Applied Design page for more information on how to compete for that award.
![](https://static.igem.org/mediawiki/2017/7/77/T--Austin_UTexas_LASA--Design1.png)
What should this page contain?
- Explanation of the engineering principles your team used in your design
- Discussion of the design iterations your team went through
- Experimental plan to test your designs
Inspiration