Difference between revisions of "Team:RDFZ-China/Results"

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<img src="https://static.igem.org/mediawiki/2017/3/32/RDFZ_Team_symbol.jpeg" alt="This is the team symbol" id="TeamSymbol">
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            <a href="https://2017.igem.org/Team:RDFZ-China"><img src="https://static.igem.org/mediawiki/2017/5/57/RDFZ_logo.png" /></a>
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        <li><a href="https://2017.igem.org/Team:RDFZ-China/Team" style = "color:white">Team</a>
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            <img src="https://static.igem.org/mediawiki/2017/6/6e/RDFZ_wiki_nav.png" />
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          <ul>
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            <li><a href="https://2017.igem.org/Team:RDFZ-China/Results">Results</a></li>
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<li><a href="https://2017.igem.org/Team:RDFZ-China/Collaboration">Collobration</a></li>
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            <li><a href ="https://2017.igem.org/Team:RDFZ-China/InterLab">Interlab</a></li>
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          </ul>
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         </li>
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        <li><a href="https://2017.igem.org/Team:RDFZ-China/Description" style = "color:white" >Project </a>
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             <ul>
 
             <ul>
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              <li><a href="https://2017.igem.org/Team:RDFZ-China/Description" >Description</a></li>
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                <li class="team">
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              <li><a href="https://2017.igem.org/Team:RDFZ-China/Design">Design</a></li>
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                    <a href="https://2017.igem.org/Team:RDFZ-China/Team">Team</a>
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              <li><a href="https://2017.igem.org/Team:RDFZ-China/Experiments">Experiments</a></li>
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                    <ul>
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              <li><a href="https://2017.igem.org/Team:RDFZ-China/Parts" style = "color:white">Parts</a></li>
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                        <li><a href="https://2017.igem.org/Team:RDFZ-China/Attributions">Attribution</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:RDFZ-China/Results">Results</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:RDFZ-China/Safety">Safety</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:RDFZ-China/InterLab">Interlab</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:RDFZ-China/Notebook">Notebook</a></li>
 +
                    </ul>
 +
                </li>
 +
                <li class="project">
 +
                    <a href="https://2017.igem.org/Team:RDFZ-China/Description">Project </a>
 +
                    <ul>
 +
                        <li><a href="https://2017.igem.org/Team:RDFZ-China/Description">Description</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:RDFZ-China/Design">Design</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:RDFZ-China/Experiments">Experiments</a></li>
 +
           
 +
                    </ul>
 +
                </li>
 +
                <li class="awards">
 +
                    <a href="https://2017.igem.org/Team:RDFZ-China/Applied_Design">Awards</a>
 +
                    <ul>
 +
                        <li><a href="https://2017.igem.org/Team:RDFZ-China/Entrepreneurship">Entrepreneurship</a></li>
 +
                    </ul>
 +
                </li>
 +
                <li class="parts"><a href="https://2017.igem.org/Team:RDFZ-China/Parts">Parts</a>
 +
                <ul>
 +
                <li><a href="https://2017.igem.org/Team:RDFZ-China/Basic_Part">Basic Parts</a></li>
 +
                <li><a href="https://2017.igem.org/Team:RDFZ-China/Composite_Part">Composite Parts</a></li>
 +
                </ul>
 +
                </li>
 +
                <li class="human">
 +
                    <a href="https://2017.igem.org/Team:RDFZ-China/HP/Silver">Human<br>Practice</a>
 +
                    <ul>
 +
                        <li><a href="https://2017.igem.org/Team:RDFZ-China/HP/Silver">HP Silver</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:RDFZ-China/HP/Gold_Integrated">HP Gold</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:RDFZ-China/Engagement">Public Engagament</a></li>
 +
                        <li><a href="https://2017.igem.org/Team:RDFZ-China/Collaboration">Collobration</a></li>
 +
                    </ul>
 +
                </li>
 +
                <li class="model"><a href="https://2017.igem.org/Team:RDFZ-China/Model">Model</a>
 +
                <ul>
 +
                <li><a href="https://2017.igem.org/Team:RDFZ-China/Hardware">Hardware</a></li>
 +
                </ul>
 +
                </li>
 
             </ul>
 
             </ul>
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         </li>
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         </nav>
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         <li><a href="https://2017.igem.org/Team:RDFZ-China/Model" style = "color:white">Model</a></li>  
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         <div style="clear:both;"></div>
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        <li><a href="https://2017.igem.org/Team:RDFZ-China/Safety" style = "color:white">Safety</a></li>  
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    </header>
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        <li><a href="https://2017.igem.org/Team:RDFZ-China/HP/Silver" style = "color:white; word-spacing:5px;">Human Practice</a>
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    <main>
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            <ul>
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        <!--Title-->
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            <li><a href="https://2017.igem.org/Team:RDFZ-China/Notebook">Notebook</a></li>
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      <section class="title">
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            </ul>
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            <div class="title-content">
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         </li>  
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                <img src="https://static.igem.org/mediawiki/2017/c/c0/Rdfz_wiki_results.png" class="m"/>
−
         <li><a href="https://2017.igem.org/Team:RDFZ-China/Applied_Design" style = "color:white">Awards</a>
+
           
−
            <ul>
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            <h1>Achievements</h1>
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            <li><a href="https://2017.igem.org/Team:RDFZ-China/Model">Model</a></li>
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                <hr class= "line">
−
            <li><a href="https://2017.igem.org/Team:RDFZ-China/Entrepreneurship">Entrepreneurship</a></li>
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                <p>We have successful results:</p>
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             </ul>
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                <ul>
−
         </li>  
+
                    <li>We helped to generate the data for the Interlab study this year.</li>
−
          
+
                    <li>We isolated two full-length genes - sfp and YerP from our Bacillus subtilis strain and successfully processed them into biobricks, and the sequencing results are positive.</li>
 +
                    <li>We ligated lmrA into pHT01, a dual host, expression vector for Bacillus subtilis, and the sequencing result is positive.
 +
We measured the growth curve for our strain and tested two different transformation protocols.</li>
 +
                    <li>We assembled our surfactin-producing operon by overlap extension PCR, and the sequencing results are positive.</li>
 +
                    <li>We constructed a mathematical model and applied various estimations and confirmed parameters to obtain useful predictions.
 +
</li>
 +
                    <li>We designed and constructed a sprinkler model that can be used to spray bacterial suspensions for bioremediation.</li>
 +
                    <li>We adapted a protocol to measure leaching efficiency of different eluents in the soil quantitatively.</li>
 +
                </ul>
 +
                <p></p>
 +
                <p>But we also have unsuccessful results:</p>
 +
                <ul>
 +
                    <li>We failed to ligate many of our biobricks into pSB1C3 at various times, which may be due to low ligation efficiency in our lab.</li>
 +
                    <li>We didn’t do much characterization for our parts.</li>
 +
                    <li>We didn’t have time to test for surfactin production by our engineered strain.</li>
 +
                    <li>We didn’t construct our designed integration vector for B. subtilis, although we had prepared the fragments for Gibson assembly.</li>
 +
                    <li>We had a difficult time of transforming B. subtilis in our lab, and we once contaminated the competent cells.</li>
 +
                </ul>
 +
                <p></p>
 +
            <h1>Future Plans</h1>
 +
                <hr class= "line">
 +
                <ul>
 +
                    <li>We will complete the construction of our integration vector and surfactin-producing operon.</li>
 +
                    <li>We will test for the efficiency of surfactin production by analytical methods.</li>
 +
                    <li>We will refine our protocol for testing leaching efficiency and test our strain’s ability to emulsify the oil.</li>
 +
                    <li>We will analyze the eluate to see if the microbiome degrades the hydrocarbons.</li>
 +
                    <li>We will deposit our strain in a real-world contamination zone and see if it works.</li>  
 +
                    <li>We will continue to analyze possible interactions between lmrA and surfactin as its substrate; then we will find mutated variants that could export surfactin more efficiently.</li>
 +
                </ul>
 +
         </div>
 +
        </section>
 +
        <footer>
 +
      <ul class="x">
 +
         <li><img src="https://static.igem.org/mediawiki/2017/7/76/Rdfz_wiki_footer.png" class="math"/></li>
 +
        <li><img src="https://static.igem.org/mediawiki/2017/5/57/RDFZ_logo.png" class="footer-logo"/></li>
 +
        <li><img src="https://static.igem.org/mediawiki/2017/1/1a/T--Bristol--igem.png" class="igem"/></li>
 +
      </ul>
 +
      <div class="fp">
 +
             <img src="https://static.igem.org/mediawiki/2017/3/3c/Rdfz_wiki_footer_image.png" class="fb">
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         </footer>
 
          
 
          
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<h1>Results</h1>
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<p>Here you can describe the results of your project and your future plans. </p>
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<h5>What should this page contain?</h5>
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<ul>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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<h5>You should also describe what your results mean: </h5>
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<ul>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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<h5> Project Achievements </h5>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<h5>Inspiration</h5>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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Revision as of 06:51, 1 November 2017

RDFZ-China

Achievements

We have successful results:

  • We helped to generate the data for the Interlab study this year.
  • We isolated two full-length genes - sfp and YerP from our Bacillus subtilis strain and successfully processed them into biobricks, and the sequencing results are positive.
  • We ligated lmrA into pHT01, a dual host, expression vector for Bacillus subtilis, and the sequencing result is positive. We measured the growth curve for our strain and tested two different transformation protocols.
  • We assembled our surfactin-producing operon by overlap extension PCR, and the sequencing results are positive.
  • We constructed a mathematical model and applied various estimations and confirmed parameters to obtain useful predictions.
  • We designed and constructed a sprinkler model that can be used to spray bacterial suspensions for bioremediation.
  • We adapted a protocol to measure leaching efficiency of different eluents in the soil quantitatively.

But we also have unsuccessful results:

  • We failed to ligate many of our biobricks into pSB1C3 at various times, which may be due to low ligation efficiency in our lab.
  • We didn’t do much characterization for our parts.
  • We didn’t have time to test for surfactin production by our engineered strain.
  • We didn’t construct our designed integration vector for B. subtilis, although we had prepared the fragments for Gibson assembly.
  • We had a difficult time of transforming B. subtilis in our lab, and we once contaminated the competent cells.

Future Plans

  • We will complete the construction of our integration vector and surfactin-producing operon.
  • We will test for the efficiency of surfactin production by analytical methods.
  • We will refine our protocol for testing leaching efficiency and test our strain’s ability to emulsify the oil.
  • We will analyze the eluate to see if the microbiome degrades the hydrocarbons.
  • We will deposit our strain in a real-world contamination zone and see if it works.
  • We will continue to analyze possible interactions between lmrA and surfactin as its substrate; then we will find mutated variants that could export surfactin more efficiently.