Difference between revisions of "Team:Grenoble-Alpes/Protocols"

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         <h2 class="titre_categorie" id="cat1" style="padding-bottom: 2%; background:#27ae60; margin:0%;"> Categorie 1 </h2>
 
         <h2 class="titre_categorie" id="cat1" style="padding-bottom: 2%; background:#27ae60; margin:0%;"> Categorie 1 </h2>
  
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               <a href="#protocol1" style="text-decoration: none;"><h2 class="titre_protocole"> LB Broth </h2></a>
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               <a href="#protocol1" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
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<a href="#protocol2" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
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<a href="#protocol3" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
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<a href="#protocol1" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
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<a href="#protocol1" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a>
 
         </div>
 
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         <div id="protocol1">
 
         <div id="protocol1">

Revision as of 07:13, 29 August 2017

Lab

Protocols

Categorie 1

Materials :
  • 20g LB Broth Base (powder)
  • 1L Distillated Water
    • Methods :
    1. Add 20g of LB Broth Base per 1L of distillated water
    2. Homogenize
    3. Autoclave at 121°C for 15 minutes

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    Materials :
  • 32g LB Agar (powder)
  • 1L Distillated Water
    • Methods :
    1. Add 32g of LB Agar per 1L of distillated water
    2. Homogenize
    3. Autoclave at 121°C for 15 minutes

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    Materials :
  • 1g Agarose (powder)
  • 100mL TAE
  • Distillated Water
  • 25uL Gel Red
    • Methods :
    1. Gel preparation
      1. Dissolve 1g of agarose in 100mL of TAE (1X)
      2. Pour the solution into the gel mould
      3. Let the solution gel (almost 15 minutes)
    2. Preparation of the samples to deposit
    3. The migration is done at 100V during 30 minutes
    4. Revelation
      1. Prepare 250mL of distillated water in a tank
      2. Add 25uL of Gel Red. Do not forget to use gloves to manipulate the Gel Red.
      3. Incubate the gel in the solution for 10 minutes at the obscurity
      4. Wash the gel in a tank of distillated water

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    Categorie 2

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    Categorie 3

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