Difference between revisions of "Team:Grenoble-Alpes/Protocols"
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<a href="#protocol1" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a> | <a href="#protocol1" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a> | ||
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Materials : | Materials : | ||
<li>20g LB Broth Base (powder)</li> | <li>20g LB Broth Base (powder)</li> | ||
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<li>Autoclave at 121°C for 15 minutes</li> | <li>Autoclave at 121°C for 15 minutes</li> | ||
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<a href="#cat1" style="text-decoration: none;"><h2 class="top">TOP</h2></a> | <a href="#cat1" style="text-decoration: none;"><h2 class="top">TOP</h2></a> | ||
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Materials : | Materials : | ||
<li>32g LB Agar (powder)</li> | <li>32g LB Agar (powder)</li> | ||
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<li>Autoclave at 121°C for 15 minutes</li> | <li>Autoclave at 121°C for 15 minutes</li> | ||
</ol> | </ol> | ||
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<a href="#cat1" style="text-decoration: none;"><h2 class="top">TOP</h2></a> | <a href="#cat1" style="text-decoration: none;"><h2 class="top">TOP</h2></a> | ||
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Materials : | Materials : | ||
<li>1g Agarose (powder)</li> | <li>1g Agarose (powder)</li> | ||
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</ol> | </ol> | ||
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<a href="#cat1" style="text-decoration: none;"><h2 class="top">TOP</h2></a> | <a href="#cat1" style="text-decoration: none;"><h2 class="top">TOP</h2></a> | ||
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<h2 class="titre_categorie" id="cat2" style="padding-bottom: 2%; background:#27ae60; margin:0%;"> Categorie 2 </h2> | <h2 class="titre_categorie" id="cat2" style="padding-bottom: 2%; background:#27ae60; margin:0%;"> Categorie 2 </h2> | ||
Revision as of 07:27, 29 August 2017
Protocols
Categorie 1
Materials :
- Add 20g of LB Broth Base per 1L of distillated water
- Homogenize
- Autoclave at 121°C for 15 minutes
TOP
Materials :
- Add 32g of LB Agar per 1L of distillated water
- Homogenize
- Autoclave at 121°C for 15 minutes
TOP
Materials :
- Gel preparation
- Dissolve 1g of agarose in 100mL of TAE (1X)
- Pour the solution into the gel mould
- Let the solution gel (almost 15 minutes)
- Preparation of the samples to deposit
- The migration is done at 100V during 30 minutes
- Revelation
- Prepare 250mL of distillated water in a tank
- Add 25uL of Gel Red. Do not forget to use gloves to manipulate the Gel Red.
- Incubate the gel in the solution for 10 minutes at the obscurity
- Wash the gel in a tank of distillated water
TOP
Categorie 2
blablabla
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blablabla
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blablabla
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Categorie 3
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