Difference between revisions of "Team:Grenoble-Alpes/Protocols"
| Line 77: | Line 77: | ||
} | } | ||
| − | #protocol1:target, #protocol2:target, #protocol3:target, #protocol4:target, #protocol5:target, #protocol6:target, #protocol7:target, #protocol8:target, #protocol9:target { | + | #protocol1:target:before, #protocol2:target, #protocol3:target, #protocol4:target, #protocol5:target, #protocol6:target, #protocol7:target, #protocol8:target, #protocol9:target { |
display: block; | display: block; | ||
-webkit-animation: fadeIn 1s; | -webkit-animation: fadeIn 1s; | ||
| Line 125: | Line 125: | ||
<div style="width:100%;"> | <div style="width:100%;"> | ||
<a href="#protocol1" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a> | <a href="#protocol1" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a> | ||
| − | <a href="#protocol2" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a> | + | <a href="#protocol2" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a> |
| − | <a href="#protocol3" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a> | + | <a href="#protocol3" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a> |
| − | <a href="#protocol1" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a> | + | <a href="#protocol1" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a> |
| − | <a href="#protocol1" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a> | + | <a href="#protocol1" style="text-decoration: none;"> <img class="logo" src="https://static.igem.org/mediawiki/2017/0/06/Lab_2.png"></a> |
</div> | </div> | ||
Revision as of 07:35, 29 August 2017
Protocols
Categorie 1
Materials :
- Add 20g of LB Broth Base per 1L of distillated water
- Homogenize
- Autoclave at 121°C for 15 minutes
TOP
Materials :
- Add 32g of LB Agar per 1L of distillated water
- Homogenize
- Autoclave at 121°C for 15 minutes
TOP
Materials :
- Gel preparation
- Dissolve 1g of agarose in 100mL of TAE (1X)
- Pour the solution into the gel mould
- Let the solution gel (almost 15 minutes)
- Preparation of the samples to deposit
- The migration is done at 100V during 30 minutes
- Revelation
- Prepare 250mL of distillated water in a tank
- Add 25uL of Gel Red. Do not forget to use gloves to manipulate the Gel Red.
- Incubate the gel in the solution for 10 minutes at the obscurity
- Wash the gel in a tank of distillated water
TOP
Categorie 2
blablabla
TOP
blablabla
TOP
blablabla
TOP
Categorie 3
blablabla
TOP
blablabla
TOP
blablabla
TOP
