Difference between revisions of "Team:Grenoble-Alpes/Protocols"
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Revision as of 07:39, 29 August 2017
Protocols
Categorie 1
Materials :
- Add 20g of LB Broth Base per 1L of distillated water
- Homogenize
- Autoclave at 121°C for 15 minutes
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Materials :
- Add 32g of LB Agar per 1L of distillated water
- Homogenize
- Autoclave at 121°C for 15 minutes
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Materials :
- Gel preparation
- Dissolve 1g of agarose in 100mL of TAE (1X)
- Pour the solution into the gel mould
- Let the solution gel (almost 15 minutes)
- Preparation of the samples to deposit
- The migration is done at 100V during 30 minutes
- Revelation
- Prepare 250mL of distillated water in a tank
- Add 25uL of Gel Red. Do not forget to use gloves to manipulate the Gel Red.
- Incubate the gel in the solution for 10 minutes at the obscurity
- Wash the gel in a tank of distillated water
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Categorie 2
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Categorie 3
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