Difference between revisions of "Team:HK SKHLPSS"
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This year, the ultimate goal of our iGEM team is trying to establish a metal ion responsive molecular beacon in E. coli as a general platform for detecting environmental contamination using bacteria. In order to achieve this progressively, we will first perform the experiment in vitro using molecular beacon that consists of short oligos as a prove of concept. Then we will try to express the system in E. coli and conduct the same experiment using laboratory prepared sample and on-site collected sample for the detection of contamination. | This year, the ultimate goal of our iGEM team is trying to establish a metal ion responsive molecular beacon in E. coli as a general platform for detecting environmental contamination using bacteria. In order to achieve this progressively, we will first perform the experiment in vitro using molecular beacon that consists of short oligos as a prove of concept. Then we will try to express the system in E. coli and conduct the same experiment using laboratory prepared sample and on-site collected sample for the detection of contamination. | ||
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Revision as of 06:41, 4 October 2017
Project Description
A Self-Assembled DNA Nanocube for the Diagnosis of H3N2 Influenza
DNA is always used a genetic material for information transfer over generations. Its programmability also allows the application in fabricating various objects for diagnostics and therapeutics as the field of DNA nanotechnology. This year, we used DNA to fabricate a three-dimensional cube that is responsive to the presence of H3N2 influenza mRNA biomarker. We used DNA and RNA oligos of the same sequence of the target for detection. From the result, we found that the DNA nanocube specifically responses to the presence of target leading to the opening of the lid. This results in the disassemble of the quadruplex formation and reduction in hemin-mediated peroxidase activity. We proved that a three-dimensional nanodevice can be used for quick diagnosis within 30 minutes and it is applicable for the detection of different biomarkers and we wish to largely produce this diagnostic device with engineered bacteria.
