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| − | <h2 style="padding-top:2%; padding-bottom:2%;"> Characterisation of BBa_J04450 and BBa_K801100: </h2>
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| − | <h5>
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| − | This year, we worked on BBa_J04450 and BBa_K801100 and searched the importance of IPTG induction on the production of mRFP1 so as to use this part in our kit.
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| − | It was essential for us to know if there is an importance of the inducer in RFP production. A strong leak of RFP production is observed and could be seen on appropriate wavelength.
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| − | <img style="width:65%; display: block; margin-left: auto; margin-right: auto ; padding: 3% 3% 0.5% 3%" src="https://static.igem.org/mediawiki/2017/0/05/Comparaison_fluo_igem_grenoble.png">
| + | <div style="background:#27ae60; margin:0%; padding-bottom: 1%;"><h2 class="titre_categorie" id="prep" > Step 1 : Target Preparation </h2></div> |
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| − | A : Tubes used for B&C photos. Photos were taken with SnapLab, the detection kit made by Grenoble-Alpes iGEM Team (see pictures below).
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| − | <img style="width:65%; display: block; margin-left: auto; margin-right: auto ; padding: 1% 3% 3% 3%" src="https://static.igem.org/mediawiki/2017/5/5a/Boitier_igem_grenoble.png">
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| − | We focused our research on the estimation of a difference of fluorescence intensity and a quicker induction, if it exists.
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| − | <img style="width:60%; display: block; margin-left: auto; margin-right: auto" src="https://static.igem.org/mediawiki/2017/1/1d/Evolution_ratio_plasmides_igem_grenoble.png">
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| − | <img style="width:60%; display: block; margin-left: auto; margin-right: auto; padding : 0% 0% 2% 0%" src="https://static.igem.org/mediawiki/2017/4/48/Results_fluo_parts_igem_grenoble_2.png">
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| − | <h5 style="text-align:center">
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| − | Results showed IPTG had an importance in RFP production. Indeed, we can see that IPTG allow an augmentation of RFP after a decrease period.
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| − | </h5>
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| − | </div>
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| − | <img style="width:60%; display: block; margin-left: auto; margin-right: auto; padding : 2% 0% 0% 0%" src="https://static.igem.org/mediawiki/2017/1/16/Evolution_temporelle_fluo_igem_grenoble_4.png">
| + | <div style="background:#27ae60; margin:0%; padding-bottom: 1%;"><h2 class="titre_categorie" id="prep" > Step 2 : </h2></div> |
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| | + | <div style="background:#27ae60; margin:0%; padding-bottom: 1%;"><h2 class="titre_categorie" id="prep" > Step 3 : </h2></div> |
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| − | <h5>
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| − | Results showed IPTG had an importance in RFP production. Indeed, we can see that IPTG allow an augmentation of RFP after a decrease period. We design this experience with an unique IPTG concentration, however, it could be interesting to repeat this experience with different IPTG gradient.
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Facing back to the Etendard glacier, in between the peaks of Maurienne and the ones of Oisan. 