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+ | <span dir="auto">Team:SCU-WestChina/Background</span> | ||
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+ | |||
+ | <title>index</title> | ||
+ | </head> | ||
+ | |||
+ | <body> | ||
+ | <ul class="nav nav-pills"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/b/b9/T--SCU-WestChina--logo.png" alt="" class="logo"> | ||
+ | <div class="nav-right-wrapper"> | ||
+ | <li role="presentation"> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina">Home</a> | ||
+ | </li> | ||
+ | <li role="presentation"> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Background">Background</a> | ||
+ | </li> | ||
+ | <li role="presentation" class="dropdown"> | ||
+ | <a class="dropdown-toggle" data-toggle="dropdown" href="#" role="button"> | ||
+ | Project | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Description">Description</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Demonstrate">Proof</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Parts">Parts</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Measurement">Measurement</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Hardware">Hardware</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li role="presentation"> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Model">Modeling</a> | ||
+ | </li> | ||
+ | <li role="presentation" class="dropdown"> | ||
+ | <a class="dropdown-toggle" data-toggle="dropdown" href="#" role="button"> | ||
+ | Human Practice | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/HP/Silver">Silver</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/HP/Gold_Integrated">Gold</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Engagement">Education and Public Engagement</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Collaborations">Collaborations</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li role="presentation" class="dropdown"> | ||
+ | <a class="dropdown-toggle" data-toggle="dropdown" href="#" role="button"> | ||
+ | Notebook | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Notebook">Notebook</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Protocols">Protocols</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Medals">Medals</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li role="presentation" class="dropdown"> | ||
+ | <a class="dropdown-toggle" data-toggle="dropdown" href="#" role="button"> | ||
+ | Team | ||
+ | </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Team">Team</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Attributions">Attributions</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/Acknowledgement">Acknowledgement</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li role="presentation"> | ||
+ | <a href="https://2017.igem.org/Team:SCU-WestChina/InterLab">InterLab</a> | ||
+ | </li> | ||
+ | </div> | ||
+ | </ul> | ||
+ | <div class="main-wrapper"> | ||
+ | <div class="primary-wrapper"> | ||
+ | <div class="primary-title">Interlab</div> | ||
+ | <div class="primary-content">How close can the numbers be when fluorescence is measured all around the world? | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="mid-wrapper"> | ||
+ | <div class="title-center">Introduction</div> | ||
+ | <p> | ||
+ | Over the past three years, iGEM has advanced the frontiers of science with the biggest interlaboratory studies ever done in synthetic biology. The SCU-WestChina iGEM team is honored to participate in the Interlab study this year. | ||
+ | </p> | ||
+ | <p> | ||
+ | The aim of the Interlab study is to analyze the measurement data of fluorescence with a detailed protocol, but different lab equipment and environment. In this way, researchers are able to get an access to compare fluorescence data from laboratories all over the world, in a standardized way. | ||
+ | </p> | ||
+ | <div class="title-center">Protocol</div> | ||
+ | <p> | ||
+ | <a href="https://static.igem.org/mediawiki/2017/8/85/InterLab_2017_Plate_Reader_Protocol.pdf">InterLab 2017 Plate Reader Protocol</a> was followed. The device utilized in our Interlab experiments was BioTek Cytation™ 3. | ||
+ | </p> | ||
+ | <p> | ||
+ | For this study, following constructs are used: | ||
+ | </p> | ||
+ | <p> | ||
+ | •Negative Control: I20270 | ||
+ | </p> | ||
+ | <p> | ||
+ | •Positive Control: R0040 | ||
+ | </p> | ||
+ | <p> | ||
+ | •Test Device 1: J23101.BCD2.E0040.B0015 | ||
+ | </p> | ||
+ | <p> | ||
+ | •Test Device 2: J23106.BCD2.E0040.B0015 | ||
+ | </p> | ||
+ | <p> | ||
+ | •Test Device 3: J23117.BCD2.E0040.B0015 | ||
+ | </p> | ||
+ | <p> | ||
+ | •Test Device 4: J23101+I13504 | ||
+ | </p> | ||
+ | <p> | ||
+ | •Test Device 5: J23106+I13504 | ||
+ | </p> | ||
+ | <p> | ||
+ | •Test Device 6: J23117+I13504 | ||
+ | </p> | ||
+ | <p> | ||
+ | All devices and parts were obtained from the iGEM 2017 InterLab Distribution Kit. Devices were made with a pSB1C3 plasmid backbone and transformed into Escherichia coli DH5α. | ||
+ | </p> | ||
+ | <div class="title-center">Data & Result</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/9/98/T--SCU-WestChina--Wiki-Interlab-Fig1.png" alt=""> | ||
+ | <div class="img-describe"> | ||
+ | Fig. 1 OD600 curve for each replicate, which suggests that most devices had no influence on the replication ability of the cells, while Device 4 and Device 1 showed much lower growth curves. | ||
+ | </div> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/9/9f/T--SCU-WestChina--Wiki-Interlab-Fig2.png" alt=""> | ||
+ | <div class="img-describe"> | ||
+ | Fig. 2 GFP fluorescence data of each device and replicate measured with an excitation of 485 nm and emission of 530 nm | ||
+ | </div> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/0/0c/T--SCU-WestChina--Wiki-Interlab-Fig3.png" alt=""> | ||
+ | <div class="img-describe"> | ||
+ | Fig.3 The ratio of fluorescence to growth for each Interlab device. | ||
+ | </div> | ||
+ | <p> | ||
+ | Results from the Plate Reader indicated that the promoter of the Device 4 is strongest, followed by the promoter of the Device 2 and Device 1. | ||
+ | </p> | ||
+ | <p> | ||
+ | The lowest Intensity was detected in Device 3, Device 5 and Device 6. | ||
+ | </p> | ||
+ | </div> | ||
+ | <script src="https://code.jquery.com/jquery-3.2.1.min.js"></script> | ||
+ | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script> | ||
+ | <!-- <script src="https://cdn.bootcss.com/echarts/3.7.2/echarts.min.js"></script> --> | ||
+ | </body> | ||
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</p> | </p> | ||
− | </div> | + | <!-- |
+ | NewPP limit report | ||
+ | CPU time usage: 0.007 seconds | ||
+ | Real time usage: 0.009 seconds | ||
+ | Preprocessor visited node count: 14/1000000 | ||
+ | Preprocessor generated node count: 64/1000000 | ||
+ | Post‐expand include size: 82/2097152 bytes | ||
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Revision as of 03:14, 31 October 2017
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Over the past three years, iGEM has advanced the frontiers of science with the biggest interlaboratory studies ever done in synthetic biology. The SCU-WestChina iGEM team is honored to participate in the Interlab study this year.
The aim of the Interlab study is to analyze the measurement data of fluorescence with a detailed protocol, but different lab equipment and environment. In this way, researchers are able to get an access to compare fluorescence data from laboratories all over the world, in a standardized way.
InterLab 2017 Plate Reader Protocol was followed. The device utilized in our Interlab experiments was BioTek Cytation™ 3.
For this study, following constructs are used:
•Negative Control: I20270
•Positive Control: R0040
•Test Device 1: J23101.BCD2.E0040.B0015
•Test Device 2: J23106.BCD2.E0040.B0015
•Test Device 3: J23117.BCD2.E0040.B0015
•Test Device 4: J23101+I13504
•Test Device 5: J23106+I13504
•Test Device 6: J23117+I13504
All devices and parts were obtained from the iGEM 2017 InterLab Distribution Kit. Devices were made with a pSB1C3 plasmid backbone and transformed into Escherichia coli DH5α.
![](https://static.igem.org/mediawiki/2017/9/98/T--SCU-WestChina--Wiki-Interlab-Fig1.png)
![](https://static.igem.org/mediawiki/2017/9/9f/T--SCU-WestChina--Wiki-Interlab-Fig2.png)
![](https://static.igem.org/mediawiki/2017/0/0c/T--SCU-WestChina--Wiki-Interlab-Fig3.png)
Results from the Plate Reader indicated that the promoter of the Device 4 is strongest, followed by the promoter of the Device 2 and Device 1.
The lowest Intensity was detected in Device 3, Device 5 and Device 6.