Difference between revisions of "Team:RDFZ-China/Team"

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                 <h1>Protocols</h1>
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                 <h1>Who are we?</h1>
 
             <hr class= "line">
 
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                <h2>Sand Pack Test</h2>
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                 <p>We are Grade 11 and 12 students from the High School Affiliated to Renmin University of China, RDFZ! With high interest in and great passion for biology, especially synthetic biology, we participate in iGEM this year for the first time, representing our school. Our team is not only great at English, capable of communicate with other scholars globally, but also knowledgeable regards to synthetic biology. Top candidates in British Biology Olympiad and USA Biology Olympiad are in our team; as brilliant as our students, our instructors are highly trained, responsible and experienced, providing us important guidance technically during the project and sharing their unique experience with us to improve our experiments as well as managing the whole project.</p>
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                 <p></p>
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                 <h1>Team Members</h1>
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                 <p>Materials:</p>
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                 <hr class="line">
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                 <ol>
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                <p>Bowen Xiao<br>
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                    <li>A type of surfactant</li>
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Team leader of RDFZ-China! Grade 12 student who has a great fondness for biology and neurology. Participated in iGEM 2016 and won silver prize. Really love iGEM and plan to carry it on in university.<br><br>
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                    <li>Petroleum or sunflower oil</li>
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                    <li>Soil sample</li>
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Siqing Wang<br>
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                    <li>Tap water</li>
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In charge of human practice in our team. Have a huge interest in biological techniques, social science and medical science. Possess lots of practical experience from summer school and other biological events. Participated in social science workshop in Columbia. Won bronze prize in USABO.<br><br>
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                    <li>Plastic bottles</li>
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                    <li>Frame</li>
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Xuanyu Zhou<br>
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                </ol>
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In charge of publication in our team. Have great amount of experiences in biology competitions, including Dongrun-Yau Science Award and BBO. Outstanding in biology, English expression and debate.<br><br>
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                <p>Investigating the optimal concentration:</p>
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                <ol>
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Yuyin Yang<br>
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                    <li>Prepare plastic bottles and place them in the frame.</li>
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In charge of publication in our team. Greatly interested in synthetic biology. Won International Brain Bee second class prize. Vice-president of the 30th Student Union.<br><br>
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                    <li>Weigh about 200g of soil sample, and fill them into the plastic bottle.</li>
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                    <li>Measure 20ml of oil with a measuring cylinder, and add them to each soil sample.</li>
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Kaiying Shan<br>
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                    <li>Wait for 2hrs to allow the oil to combine with the soil.</li>
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In charge of theoretical design of the experiment and project. Have a great passion and fondness for biology. Won golden prize in Biology and Chemistry Olympiad.<br><br>
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                    <li>Prepare surfactant solutions with different concentrations.</li>
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                    <li>Add 20ml of surfactant solutions to the oil.</li>
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Kexin Zhu<br>
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                    <li>Place a cup under each bottle. Wait for 2hrs for the emulsion to occur.</li>
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In charge of human practice in our team. Highly interested in synthetic biology. Have some practical skills in laboratory.<br><br>
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                    <li>Carefully cover the cups and freeze them in -20℃.</li>
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                    <li>After the liquid freezes, separate the ice and soil residue from the remaining liquid by filtering. The volume of liquid should be equal to the volume of oil eluded from the soil. Record it in the table below.</li>
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Rui Deng<br>
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                    <li>Calculate the percentage elusion with (V/20ml)*100%.</li>
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In charge of collecting data. Love biology and digging into books and papers.<br><br>
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                </ol>
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                <p>THERE SHOULD BE A PICTURE OF THE TABLE FOR DATA</p>
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Yiqing Zhang<br>
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                <h2>OEPCR(Overlap Extension PCR)</h2>
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In charge of design and publication. Participate in research for the main subject of our project and the function of Bacillus subtilis. Record every meeting and create documents on our official account.<br><br>
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                <p></p>
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−
                <p>Materials:</p>
+
Junhao Yang<br>
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                <ol>
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In charge of human practice. Interested in biology and won prize in BBO.<br><br>
−
                    <li>Template for the left and right fragment</li>
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                    <li>Primers A,B,C,D.</li>
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Zeyu Tang<br>
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                    <li>2x Pfu mastermix</li>
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The coordinator of our team. Meanwhile, in charge of wiki and modeling. Really intrigued by biology and won prize in BBO and USABO. Have some practical experience of experiment.<br><br>
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                    <li>DNA purification kit</li>
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                </ol>
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Yixin Shen<br>
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                <p></p>
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In charge of publication and wiki design. Enthusiastic of biology. Internship in Department of Medicine in Peking University. Have an outstanding oral ability in English. Have a professional training of art for 9 years, great in aesthetic judgment.<br><br>
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                <p>OEPCR Steps:</p>
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−
                <ol>
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Yuze Cao<br>
−
                    <li>Amplify desired DNA fragments on left and right template with AB and CD primer pairs correspondingly. The annealing temperature is set to 55°C. When designing the primers, B is made to be complementary to C, with 30 bases overlapping with ends of the left and right fragments to be joined. (Make Tm be as close to 60°C as possible.)</li>
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In charge of modeling and wiki. Possess solid basis of biology studies and great experimental techniques. Biology lover and won silver prize in BBO.<br><br>
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                    <li>Directly purify PCR product with a kit. Elute DNA with ddH2O.</li>
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−
                    <li>Set a new PCR reaction with specific requirements: equimolar amount of left and right fragment PCR product from step 1, combined volume of the fragments should be 1/2 to 3/4 of the total volume, no new primers is added, annealing temperature is set to 60°C. Run PCR for 15 cycles.</li>
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Junying Wang<br>
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                    <li>Add primers AD to the previous PCR product and set annealing temperature to 72°C and run PCR for another 15-20 cycles.</li>
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In charge of wiki design and digital record (i.e. take photos). Interested in biology. The head of Publication Department of the 30th Student Union. The vice-president of the 31st Student Union.<br><br>
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                    <li>Electrophorese, and cut gels of correct product length and recycle OEPCR product.</li>
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                </ol>
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Ziyuan Zhao<br>
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                <p>(If the amount of OEPCR product is not enough for later experiments, consider amplifying the product with AD primers, but be aware of nonspecific amplification results!)</p>
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In charge of the design of project and experiment. Clear about the whole competition and help the team make critical decisions. Knowledgeable in chemistry and biology, expert of molecular biology experiments. Participated in iGEM 2016. Won golden prize in BBO and USABO.<br><br>
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+
 
 +
Yuwei Guo<br>
 +
In charge of research and practical techniques in our team. Independently researched on epigenetic projects and published paper on science journal. Passion for science research, won silver prize in China Thinking Big.<br><br>
 +
 
 +
Zihan Liu<br>
 +
In charge of experiment and record. Interested in biology and have some experience of experiments.<br><br>
 +
 
 +
Haining Wang<br>
 +
In charge of publicizing, crowd funding and wiki editing. Biology lover, have some experimental experience.<br><br>
 +
 
 +
<b>Instructors</b><br>
 +
Weifeng Wilson Nina<br>
 +
In charge of the whole project and the main instructor. The member of Chinese Society of Education, an expert of VR/AR education. Instruct students in school taking part in various national and international biology competitions and help them have outstanding performance. <br>
 +
“iGem might be your future!”<br><br>
 +
 
 +
Wenfeng Liu<br>
 +
In charge of laboratory and responsible for lab safety. lead and instruct research team in our school. Provide our team with professional instructions on experimental techniques.</p>
 
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Revision as of 14:06, 1 November 2017

RDFZ-China

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Who are we?

We are Grade 11 and 12 students from the High School Affiliated to Renmin University of China, RDFZ! With high interest in and great passion for biology, especially synthetic biology, we participate in iGEM this year for the first time, representing our school. Our team is not only great at English, capable of communicate with other scholars globally, but also knowledgeable regards to synthetic biology. Top candidates in British Biology Olympiad and USA Biology Olympiad are in our team; as brilliant as our students, our instructors are highly trained, responsible and experienced, providing us important guidance technically during the project and sharing their unique experience with us to improve our experiments as well as managing the whole project.

Team Members

Bowen Xiao
Team leader of RDFZ-China! Grade 12 student who has a great fondness for biology and neurology. Participated in iGEM 2016 and won silver prize. Really love iGEM and plan to carry it on in university.

Siqing Wang
In charge of human practice in our team. Have a huge interest in biological techniques, social science and medical science. Possess lots of practical experience from summer school and other biological events. Participated in social science workshop in Columbia. Won bronze prize in USABO.

Xuanyu Zhou
In charge of publication in our team. Have great amount of experiences in biology competitions, including Dongrun-Yau Science Award and BBO. Outstanding in biology, English expression and debate.

Yuyin Yang
In charge of publication in our team. Greatly interested in synthetic biology. Won International Brain Bee second class prize. Vice-president of the 30th Student Union.

Kaiying Shan
In charge of theoretical design of the experiment and project. Have a great passion and fondness for biology. Won golden prize in Biology and Chemistry Olympiad.

Kexin Zhu
In charge of human practice in our team. Highly interested in synthetic biology. Have some practical skills in laboratory.

Rui Deng
In charge of collecting data. Love biology and digging into books and papers.

Yiqing Zhang
In charge of design and publication. Participate in research for the main subject of our project and the function of Bacillus subtilis. Record every meeting and create documents on our official account.

Junhao Yang
In charge of human practice. Interested in biology and won prize in BBO.

Zeyu Tang
The coordinator of our team. Meanwhile, in charge of wiki and modeling. Really intrigued by biology and won prize in BBO and USABO. Have some practical experience of experiment.

Yixin Shen
In charge of publication and wiki design. Enthusiastic of biology. Internship in Department of Medicine in Peking University. Have an outstanding oral ability in English. Have a professional training of art for 9 years, great in aesthetic judgment.

Yuze Cao
In charge of modeling and wiki. Possess solid basis of biology studies and great experimental techniques. Biology lover and won silver prize in BBO.

Junying Wang
In charge of wiki design and digital record (i.e. take photos). Interested in biology. The head of Publication Department of the 30th Student Union. The vice-president of the 31st Student Union.

Ziyuan Zhao
In charge of the design of project and experiment. Clear about the whole competition and help the team make critical decisions. Knowledgeable in chemistry and biology, expert of molecular biology experiments. Participated in iGEM 2016. Won golden prize in BBO and USABO.

Yuwei Guo
In charge of research and practical techniques in our team. Independently researched on epigenetic projects and published paper on science journal. Passion for science research, won silver prize in China Thinking Big.

Zihan Liu
In charge of experiment and record. Interested in biology and have some experience of experiments.

Haining Wang
In charge of publicizing, crowd funding and wiki editing. Biology lover, have some experimental experience.

Instructors
Weifeng Wilson Nina
In charge of the whole project and the main instructor. The member of Chinese Society of Education, an expert of VR/AR education. Instruct students in school taking part in various national and international biology competitions and help them have outstanding performance.
“iGem might be your future!”

Wenfeng Liu
In charge of laboratory and responsible for lab safety. lead and instruct research team in our school. Provide our team with professional instructions on experimental techniques.