Team:Kingsborough NY/Design
Contents
Designing a Kill-Switch
We initially attempted to create engineered bacteria that break down nitrogenous waste effectively in wastewater treatment plant. Since our E. coli can survive in saltwater, creating a kill switch for our engineered bacteria will, in theory, prevent our bacteria from thriving outside wastewater treatment plants.
Control of Gene Expression by pDawn alone
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3.1 (K2268003): When light is present, c1 WILL NOT be produced. In theory, the expression of lacZ should be maximal. This is intended to be the non-lethal version of our kill-switch.
4.1 (K2268004): When light is present, c1 WILL NOT be produced. In theory, the expression of MazF should be maximal.
Control of Gene Expression by pDawn and supplementary cI Repressor
3.2(K2268005): When light is present with no ITPG, c1 WILL NOT be produced. In theory, maximal expression of LacZ should be produced. This is a non-lethal version of our kill switch.
4.2(K2268006): When light is present with no ITPG, c1 WILL NOT be produced. In theory, maximal expression of MazF should be produced.
Light control by pDawn
Can include here the data of pDawn expression when grown in light or darkness (optional)
