Team:UChile OpenBio-CeBiB/Results

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Results


Here are the lab results










Gel visualization of amplification of the gblocks Est2 and Est4


Gel visualization of the sequences of the second order made to IDT


Gel extraction for purification of the gBlock of the second order made to IDT


LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1-Se 1_2. It grows.


LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1-Se 2_2. It grows.


LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBS1-SEQ. It do not grows.


LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3-Est1_2. It grows.


LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3-Est2. It grows


LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3. It grows


LB and Ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1. It grows


Superior gel. It is indicated with (N °) the colony from which the plasmid comes. Staircase of 1 kB. Nomenclature: Part-N ° of colony-Plasmid to which the part is bound (Lig plasmid)


Lower gel. It is indicated with (N °) the colony from which the plasmid comes. Staircase of 1 kB. Nomenclature: Part-N ° of colony-Plasmid to which the part is bound (Lig plasmid).

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