Difference between revisions of "Team:UChile OpenBio-CeBiB/Experiments"

 
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<h1>Experiments</h1>
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<h1 style="text-align:center;">Procedure</h1>
 
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<h2> Chlamydomonas reinhardtii growth </h2>
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The main objetives of this project, at first instance, is to develope a Chlamydomonas reinhardtii strain that is able to fix more quantities of CO2 and, at second instance, once got the ability, produce recombinant proteins that the other strains are not capable to do. On the other hand, the second aim is to characterized kineticallyan adjustable promoter for vitamin B12, which was nicely shared by Dr. Alisson Smith.</p>
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<p>That is why, our team has been working on an experimental method that is explained in the next figure:</p>
  
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<p> The strain that will be utilized is cell wall deficient, the used in [1] is the strain cw15-30-derived UVM4 C. The culture medium in which they grow are TAP (solid) and TAPS (liquid). Light stimulation will be required, for which a structure capable of covering the Erlenmeyer flasks or the petri dishes in which C. reinhardtii would be growing while uniformly illuminating with the whole visible light spectrum will be designed and built. In [1] the lamp that is put into use has the following characteristics: Nano Light, 11 Watts, Dennerle, Vinningen, Germany, which provided constant light with the following details: 2500 lux, eq. 72.5 μE/m2∙s1. </p>
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<p> For the construction of calibration curves the medium to be used is TAPS without acetate. </p>
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<p> [1] Chávez, M. N., Schenck, T. L., Hopfner, U., Centeno-Cerdas, C., Somlai-Schweiger, I., Schwarz, C., ... & Nickelsen, J. (2016). Towards autotrophic tissue engineering: photosynthetic gene therapy for regeneration. Biomaterials, 75, 25-36. </p>
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<cite>Figure I: Scheme of our plan, which the wall deficient cells are UVM4 and UVM11 strain</cite>
 
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<p><i><u>TAP (Tris-Acetate-Phosphate)</u></i></p>
 
 
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<p>Source: http://www.chlamycollection.org/methods/media-recipes/tap-and-tris-minimal/</p>
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Latest revision as of 18:28, 1 November 2017

Document

Procedure

The main objetives of this project, at first instance, is to develope a Chlamydomonas reinhardtii strain that is able to fix more quantities of CO2 and, at second instance, once got the ability, produce recombinant proteins that the other strains are not capable to do. On the other hand, the second aim is to characterized kineticallyan adjustable promoter for vitamin B12, which was nicely shared by Dr. Alisson Smith.

That is why, our team has been working on an experimental method that is explained in the next figure:


Figure I: Scheme of our plan, which the wall deficient cells are UVM4 and UVM11 strain