Difference between revisions of "Team:ECUST/Parts"

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{{ECUST}}
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<h1>Parts</h1>
 
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<body>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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</div>
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<center>
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<img src="https://static.igem.org/mediawiki/2017/b/be/Parts_banner_head.png" width="100%"/>
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<br>
  
  
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<div id="wrapper" >
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<div class="section">
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<br>
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<h1>New&nbsp;Parts</h1>
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<div class="bs-example table-responsive">
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      <table class="table table-striped table-bordered table-hover">
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        <thead>
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          <tr>
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            <th>#</th>
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            <th>Name</th>
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            <th>Type</th>
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            <th>Description</th>
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            <th>Designer</th>
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            <th>Other</th>
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          </tr>
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        </thead>
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        <tbody>
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          <tr>
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            <td>1</td>
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<td><a href="http://parts.igem.org/Part:BBa_K2308001">BBa_K2308001</a></td>
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<td>composite part</td>
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<td>knock out (upstream1+downstream1)</td>
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<td>Yunpeng Dai</td>
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<td></td>
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          </tr>
  
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          <tr>
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            <td>2</td>
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<td><a href="http://parts.igem.org/Part:BBa_K2308002">BBa_K2308002</a></td>
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<td>composite part</td>
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<td>knock in (upstream2+sYFP2+downstream2)</td>
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<td>Yunpeng Dai</td>
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<td></td>
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          </tr>
  
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<div class="highlight">
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            <td>3</td>
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<h5>Note</h5>
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<td><a href="http://parts.igem.org/Part:BBa_K2308003">BBa_K2308003</a></td>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<td>basic part</td>
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</div>
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<td>sYFP2</td>
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</div>
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<td>Yunpeng Dai</td>
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<td>improvement of BBa_K864100 </td>
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          </tr>
  
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          <tr>
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            <td>4</td>
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<td><a href="http://parts.igem.org/Part:BBa_K2308004">BBa_K2308004</a></td>
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<td>composite part</td>
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<td>knock out (upstream1+promotor+Amp+downstream1)</td>
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<td>Yunpeng Dai</td>
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<td></td>
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          </tr>
  
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          <tr>
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            <td>5</td>
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<td><a href="http://parts.igem.org/Part:BBa_K2308005">BBa_K2308005</a></td>
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<td>composite part</td>
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<td>knock in (upstream2+sYFP2+promotor+Amp+downstream2)</td>
 +
<td>Yunpeng Dai</td>
 +
<td></td>
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          </tr>
  
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          <tr>
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            <td>6</td>
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<td><a href="http://parts.igem.org/Part:BBa_K2308006">BBa_K2308006</a></td>
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<td>basic part</td>
 +
<td>upstream1</td>
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<td>Yunpeng Dai</td>
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<td></td>
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          </tr>
  
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<div class="column half_size">
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          <tr>
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            <td>7</td>
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<td><a href="http://parts.igem.org/Part:BBa_K2308007">BBa_K2308007</a></td>
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<td>basic part</td>
 +
<td>downstream1</td>
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<td>Yunpeng Dai</td>
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<td></td>
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          </tr>
  
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<h5>Adding parts to the registry</h5>
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          <tr>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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            <td>8</td>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<td><a href="http://parts.igem.org/Part:BBa_K2308008">BBa_K2308008</a></td>
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</div>
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<td>basic part</td>
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<td>upstream2</td>
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<td>Yunpeng Dai</td>
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<td></td>
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          </tr>
  
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          <tr>
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            <td>9</td>
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<td><a href="http://parts.igem.org/Part:BBa_K2308009">BBa_K2308009</a></td>
 +
<td>basic part</td>
 +
<td>downstream2</td>
 +
<td>Yunpeng Dai</td>
 +
<td></td>
 +
          </tr>
  
 +
          <tr>
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            <td>10</td>
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<td><a href="http://parts.igem.org/Part:BBa_K2308010">BBa_K2308010</a></td>
 +
<td>basic part</td>
 +
<td>Amp promotor</td>
 +
<td>Yunpeng Dai</td>
 +
<td></td>
 +
          </tr>
  
 +
          <tr>
 +
            <td>11</td>
 +
<td><a href="http://parts.igem.org/Part:BBa_K2308011">BBa_K2308011</a></td>
 +
<td>basic part</td>
 +
<td>Amp coding</td>
 +
<td>Yunpeng Dai</td>
 +
<td></td>
 +
          </tr>
  
 +
          <tr>
 +
            <td>12</td>
 +
<td><a href="http://parts.igem.org/Part:BBa_K2308012">BBa_K2308012</a></td>
 +
<td>basic part</td>
 +
<td>LacIq repressor</td>
 +
<td>Yunpeng Dai</td>
 +
<td></td>
 +
          </tr>
  
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<div class="column half_size">
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          <tr>
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            <td>13</td>
 +
<td><a href="http://parts.igem.org/Part:BBa_K2308013">BBa_K2308013</a></td>
 +
<td>basic part</td>
 +
<td>LacIq spacer</td>
 +
<td>Yunpeng Dai</td>
 +
<td></td>
 +
          </tr>
  
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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          <tr>
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<p>The information needed to initially create a part on the Registry is:</p>
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            <td>14</td>
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<ul>
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<td><a href="http://parts.igem.org/Part:BBa_K2308014">BBa_K2308014</a></td>
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<li>Part Name</li>
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<td>basic part</td>
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<li>Part type</li>
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<td>LacIq promotor</td>
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<li>Creator</li>
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<td>Yunpeng Dai</td>
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<li>Sequence</li>
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<td></td>
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<li>Short Description (60 characters on what the DNA does)</li>
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          </tr>
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<li>Long Description (Longer description of what the DNA does)</li>
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−
<li>Design considerations</li>
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−
</ul>
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<p>
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          <tr>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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            <td>15</td>
 +
<td> <a href="http://parts.igem.org/Part:BBa_K2308015">BBa_K2308015</a></td>
 +
<td>basic part</td>
 +
<td>LacIq coding</td>
 +
<td>Yunpeng Dai</td>
 +
<td></td>
 +
          </tr>
  
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</div>
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  <tr>
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            <td>16</td>
 +
<td><a href="http://parts.igem.org/Part:BBa_K2308016">BBa_K2308016</a></td>
 +
<td>composite part</td>
 +
<td>LacIq regulator</td>
 +
<td>Yunpeng Dai</td>
 +
<td>improvement of BBa_v1003 </td>
 +
          </tr>
  
  
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<div class="column half_size">
 
  
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<h5>Inspiration</h5>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
  
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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        </tbody>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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    </div><!-- /example -->
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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</div>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<div class="some-padding"></div>
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<div class="some-padding"></div>
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<div class="column full_size">
 
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<h5>Part Table </h5>
 
  
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
 
  
−
<div class="highlight">
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<div class="section">
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<br><h1>Improvements</h1>
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<div class="page-header">
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<h2 id="tables">Improvement of <a href="http://parts.igem.org/Part:BBa_K864100">Part:BBa K864100</a></h2>
 +
</div>
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<div class="style1">
 +
<p>The reporter sYPF2 has been codon-optimized for <i>Rhodobacter sphaeroides 2.4.1</i> and it was successfully tested as a reporter in this chassis. We have made several measurements to show its fluorescence in <i>Rhodobacter sphaeroides 2.4.1</i> as well its properties (Fluorescence excitation/emission spectrum).</p>
 +
</div>
 +
<p><i> To learn more about the improvement ，<a href="http://parts.igem.org/Part:BBa_K2308003">please click here.</a></i></p>
 +
<div class="some-padding"></div>
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<div class="some-padding"></div>
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<div class="some-padding"></div>
  
  
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</html>
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<div class="page-header">
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<groupparts>iGEM17 ECUST</groupparts>
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<h2 id="tables">Improvement of <a href="http://parts.igem.org/Part:BBa V1003">Part:BBa V1003</a></h2>
 +
</div>
 +
<div class="style1">
 +
<p>The regulator LacIq's coding sequences are submitted in part BBa_K2308016. In our project, we used this part with Part:BBa_K2308003 together to turn <i>Rhodobacter sphaeroides 2.4.1</i> into inducible strains and successfully induced the expression of sYFP2. </p>
 +
 +
</div>
 +
<p><i> To learn more about the improvement ，<a href="http://parts.igem.org/Part:BBa_K2308016">please click here.</a></i></p>
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</div>
  
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<br><h1>Key parts</h1>
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<div class="page-header">
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<h2 id="tables">BBa_K2308002</h2>
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</div>
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<div class="style1">
 +
<p>BBa_K2308002 is an important part in our experiment. It is composed of BBa_K2308006，BBa_K2308003 and BBa_K2308007. The function of this part is to carry out the homologous recombination on the genome of <i>Rhodobacter sphaeroides 2.4.1.</i></p><br><br>
 +
  
 +
</div>
 +
<p><i>To learn more about this part ，<a href="http://parts.igem.org/Part:BBa_K2308002">please click here.</a></i></p><br><br>
  
  
 +
<div class="page-header">
 +
<h2 id="tables">BBa_K2308003</h2>
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</div>
 +
<div class="style1">
 +
<p>sYFP2(BBa_K2308003) is the key part in our experiment. It is used both for fusion expression (see BBa_K2308002) and inducible cytoplasm expression (combined with BBa_K2308016), and the host cell is <i>Rhodobacter sphaeroides 2.4.1.</i> </p><br>
 +
<p>In inducible cytoplasm expression experiment, we used part BBa_K2308016 to turn the <i>Rhodobacter sphaedoides 2.4.1</i>  into inducible strains, and IPTG was added （800 μM in final volume）when the OD<sub>700</sub> of the strain was about 0.4（grown for about 24h）.</p><br><br>
 +
 +
</div>
 +
<p><i>To learn more about this part ，<a href="http://parts.igem.org/Part:BBa_K2308003"> please click here.</a></i></p><br><br>
 +
 +
 +
<div class="page-header">
 +
<h2 id="tables">BBa_K2308016</h2>
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</div>
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<div class="style1">
 +
<p>This part contains LacIq repressor, LacIq spacer, LacIq promotor and LacIq coding sequence and is used for <i>Rhodobacter sphaeroides 2.4.1</i>, cells with the device can carry out inducible expression of the target proteins. </p><br>
 +
                                        <p>In inducible cytoplasm expression experiment, we used part BBa_K2308016 to turn the <i>Rhodobacter sphaeroides 2.4.1</i> into inducible strains, and IPTG was added （800 μM in final volume）when the OD<sub>700</sub> of the strain was about 0.4（grown for about 24h）.</p><br><br>
 +
 +
</div>
 +
<p><i> To learn more about this part ，<a href="http://parts.igem.org/Part:BBa_K2308016">please click here.</a></i></p><br><br>
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{{:Team:ECUST/footer}}

Latest revision as of 03:33, 2 November 2017



New Parts

# Name Type Description Designer Other
1 BBa_K2308001 composite part knock out (upstream1+downstream1) Yunpeng Dai
2 BBa_K2308002 composite part knock in (upstream2+sYFP2+downstream2) Yunpeng Dai
3 BBa_K2308003 basic part sYFP2 Yunpeng Dai improvement of BBa_K864100
4 BBa_K2308004 composite part knock out (upstream1+promotor+Amp+downstream1) Yunpeng Dai
5 BBa_K2308005 composite part knock in (upstream2+sYFP2+promotor+Amp+downstream2) Yunpeng Dai
6 BBa_K2308006 basic part upstream1 Yunpeng Dai
7 BBa_K2308007 basic part downstream1 Yunpeng Dai
8 BBa_K2308008 basic part upstream2 Yunpeng Dai
9 BBa_K2308009 basic part downstream2 Yunpeng Dai
10 BBa_K2308010 basic part Amp promotor Yunpeng Dai
11 BBa_K2308011 basic part Amp coding Yunpeng Dai
12 BBa_K2308012 basic part LacIq repressor Yunpeng Dai
13 BBa_K2308013 basic part LacIq spacer Yunpeng Dai
14 BBa_K2308014 basic part LacIq promotor Yunpeng Dai
15 BBa_K2308015 basic part LacIq coding Yunpeng Dai
16 BBa_K2308016 composite part LacIq regulator Yunpeng Dai improvement of BBa_v1003

Improvements

Improvement of Part:BBa K864100

The reporter sYPF2 has been codon-optimized for Rhodobacter sphaeroides 2.4.1 and it was successfully tested as a reporter in this chassis. We have made several measurements to show its fluorescence in Rhodobacter sphaeroides 2.4.1 as well its properties (Fluorescence excitation/emission spectrum).

To learn more about the improvement ，please click here.

Improvement of Part:BBa V1003

The regulator LacIq's coding sequences are submitted in part BBa_K2308016. In our project, we used this part with Part:BBa_K2308003 together to turn Rhodobacter sphaeroides 2.4.1 into inducible strains and successfully induced the expression of sYFP2.

To learn more about the improvement ，please click here.


Key parts

BBa_K2308002

BBa_K2308002 is an important part in our experiment. It is composed of BBa_K2308006，BBa_K2308003 and BBa_K2308007. The function of this part is to carry out the homologous recombination on the genome of Rhodobacter sphaeroides 2.4.1.



To learn more about this part ，please click here.



BBa_K2308003

sYFP2(BBa_K2308003) is the key part in our experiment. It is used both for fusion expression (see BBa_K2308002) and inducible cytoplasm expression (combined with BBa_K2308016), and the host cell is Rhodobacter sphaeroides 2.4.1.


In inducible cytoplasm expression experiment, we used part BBa_K2308016 to turn the Rhodobacter sphaedoides 2.4.1 into inducible strains, and IPTG was added （800 μM in final volume）when the OD700 of the strain was about 0.4（grown for about 24h）.



To learn more about this part ， please click here.



BBa_K2308016

This part contains LacIq repressor, LacIq spacer, LacIq promotor and LacIq coding sequence and is used for Rhodobacter sphaeroides 2.4.1, cells with the device can carry out inducible expression of the target proteins.


In inducible cytoplasm expression experiment, we used part BBa_K2308016 to turn the Rhodobacter sphaeroides 2.4.1 into inducible strains, and IPTG was added （800 μM in final volume）when the OD700 of the strain was about 0.4（grown for about 24h）.



To learn more about this part ，please click here.