Team:ECUST/Parts
New Parts
| # | Name | Type | Description | Designer | Other |
|---|---|---|---|---|---|
| 1 | BBa_K2308001 | composite part | knock out (upstream1+downstream1) | Yunpeng Dai | |
| 2 | BBa_K2308002 | composite part | knock in (upstream2+sYFP2+downstream2) | Yunpeng Dai | |
| 3 | BBa_K2308003 | basic part | sYFP2 | Yunpeng Dai | improvement of BBa_K864100 |
| 4 | BBa_K2308004 | composite part | knock out (upstream1+promotor+Amp+downstream1) | Yunpeng Dai | |
| 5 | BBa_K2308005 | composite part | knock in (upstream2+sYFP2+promotor+Amp+downstream2) | Yunpeng Dai | |
| 6 | BBa_K2308006 | basic part | upstream1 | Yunpeng Dai | |
| 7 | BBa_K2308007 | basic part | downstream1 | Yunpeng Dai | |
| 8 | BBa_K2308008 | basic part | upstream2 | Yunpeng Dai | |
| 9 | BBa_K2308009 | basic part | downstream2 | Yunpeng Dai | |
| 10 | BBa_K2308010 | basic part | Amp promotor | Yunpeng Dai | |
| 11 | BBa_K2308011 | basic part | Amp coding | Yunpeng Dai | |
| 12 | BBa_K2308012 | basic part | LacIq repressor | Yunpeng Dai | |
| 13 | BBa_K2308013 | basic part | LacIq spacer | Yunpeng Dai | |
| 14 | BBa_K2308014 | basic part | LacIq promotor | Yunpeng Dai | |
| 15 | BBa_K2308015 | basic part | LacIq coding | Yunpeng Dai | |
| 16 | BBa_K2308016 | composite part | LacIq regulator | Yunpeng Dai | improvement of BBa_v1003 |
Improvements
Improvement of Part:BBa K864100
The reporter sYPF2 has been codon-optimized for Rhodobacter sphaeroides 2.4.1 and it was successfully tested as a reporter in this chassis. We have made several measurements to show its fluorescence in Rhodobacter sphaeroides 2.4.1 as well its properties (Fluorescence excitation/emission spectrum).
To learn more about the improvement ，please click here.
Improvement of Part:BBa V1003
The regulator LacIq's coding sequences are submitted in part BBa_K2308016. In our project, we used this part with Part:BBa_K2308003 together to turn Rhodobacter sphaeroides 2.4.1 into inducible strains and successfully induced the expression of sYFP2.
To learn more about the improvement ，please click here.
Key parts
BBa_K2308002
BBa_K2308002 is an important part in our experiment. It is composed of BBa_K2308006，BBa_K2308003 and BBa_K2308007. The function of this part is to carry out the homologous recombination on the genome of Rhodobacter sphaeroides 2.4.1.
To learn more about this part ，please click here.
BBa_K2308003
sYFP2(BBa_K2308003) is the key part in our experiment. It is used both for fusion expression (see BBa_K2308002) and inducible cytoplasm expression (combined with BBa_K2308016), and the host cell is Rhodobacter sphaeroides 2.4.1.
In inducible cytoplasm expression experiment, we used part BBa_K2308016 to turn the Rhodobacter sphaedoides 2.4.1 into inducible strains, and IPTG was added （800 μM in final volume）when the OD700 of the strain was about 0.4（grown for about 24h）.
To learn more about this part ， please click here.
BBa_K2308016
This part contains LacIq repressor, LacIq spacer, LacIq promotor and LacIq coding sequence and is used for Rhodobacter sphaeroides 2.4.1, cells with the device can carry out inducible expression of the target proteins.
In inducible cytoplasm expression experiment, we used part BBa_K2308016 to turn the Rhodobacter sphaeroides 2.4.1 into inducible strains, and IPTG was added （800 μM in final volume）when the OD700 of the strain was about 0.4（grown for about 24h）.
To learn more about this part ，please click here.
