Team:ECUST/Lab/Notebook
We have built the team since March 29 this year, one of Team-ECUST Teams. There are initial seven people only, which then gradually expanded to 13 people, during 7 month.
We knew nothing at the beginning of the IGEM, through step-by-step understanding and pondering, in the efforts of the team members and with the help of teachers and seniors, we accomplished the whole project finally.
Here are our team memorabilia from our team building to the wiki freeze.
2017.3.29-2017.4.5
1、Team-ECUST was established, initially 12 students were divided into two teams, our team initial members included Weijie Chen, Yunpeng Dai, Ye Li, Yuyao Jin, Yuanyuan Li, Binjie zhang and Zhenzhou Jiang.
2、We made researches about previous iGEM teams, wondering who did what.
2017.4.5-2017.4.12
After the teachers' review, we determined that the primary direction was the photosynthesis of microorganisms.
2017.4.12-2017.4.19
1、We have looked for kinds of ways to enhance the efficiency of photosynthesis and ultimately determined to broaden the absorption spectrum.
2、Under the recommendation of teachers, we selected Rhodobacter sphaeroides as a chassis biology.
2017.4.19-2017.4.26
1、Thanks to Dr. Haoqian Zhang from Beijing who shared his experience about iGEM and proposed improvements to our project.
2、So far we have determined to broaden the absorption spectra by means of fluorescence resonance energy transformation and studied its application.
2017.4.26-2017.5.3
1、In-depth study of light complexes of Rhodobacter sphaeroide and preparation of preliminary modelling.
2、plan and prepare the Human practice.
3、Jianchang Huang joined our team .
2017.5.3-2017.5.10
1、The design of the initial experimental protocol after survey lots of literature.
2、With the help of Teacher Lu, the protocol of the photo-reactor was designed preliminarily.
2017.5.10-2017.5.17
1、In this regular discussion, we and the teachers approved of the feasibility of our protocol.
2、Continued to deepen our project from all angles.
3、Light-harvester team was established, and had our own logo.
2017.5.17-2017.5.24
Kaiyu Yin, from the Hoa Bioengineering Institute, introduced us to the Gibson Assembly and gene knockout/typing method.
2017.5.24-2017.5.31
Prof. Gaoyi Tan explained some of the characteristics of Rhodobacter sphaeroide, and Xian Liu teached us how to cultivate Rhodobacter sphaeroide.
2017.5.31-2017.6.7
1、We have studied the primer design and designed the primers for the our project.
2、We studied the characteristics of Rhodobacter sphaeroide, and found that they can degrade organic matter and produce hydrogen through photosynthesis.
3、Mengqing Zhou，Lu Lu joined our team.
2017.6.7-2017.6.14
1、We have studied the specific mechanism of hydrogen production about Rhodobacter sphaeroide.
2、Investigated the requirements of iGEM medals and improved our project according to the standards.
3、We begun to cultivate Rhodobacter sphaeroide.
2017.6.14-2017.6.21
1、Reserving the reagents required for the experiment.
2、We designed postcards and mailed it to the University from German.
2017.6.21-2017.6.28
1、Completed a series of tasks according to the requirements of the iGEM official calendar.
2、Zhi Yi joined our team.
2017.6.28-2017.7.5
1、Jianan Shi，Fengting Lijoined our team.
2、We made the final preparations for the summer experiment.
2017.7.5-2017.7.12
We did the wet lab work and successfully constructed the Konck out plasmids.
2017.7.12-2017.7.19
1、We successfully constructed the Knock in plasmid.
2、We tried to reconstruct the pIND4 inducible plasmid, but the effect is poor.
3,、We produced a brochure about our team.
2017.7.19-2017.7.26
1、We conducted joint transformation experiments of Rhodobacter sphaeroide and did not achieve the predicted effect.
2、Tried to build pIND4 with Gibson assembly.
3、We completed all the experiments on Interlab.
4、We finally determined the scheme of the luminous stirring paddle, and discussed the details.
2017.7.26-2017.8.2
1、We conducted joint transformation experiments of Rhodobacter sphaeroide and did not achieve the predicted effect.
2、We successsfully assembled and constructed pIND4 plasmids by Gibson.
3、Cooperated with the iGEM team from SJTU.
4、We use CAD software to plot the paddle.
2017.8.2-2017.8.9
1、We transform Rhodobacter sphaeroide by electric shock, but the effect is not good.
2、We successfully constructed PIND4-SYFP2 (optimized) plasmids.
3、Measured the growth curve of Rhodobacter sphaeroides.
4、Thanks to the Synthetic Biology Gene Company as our sponsor, we made team jackets, brochure and postcard.
2017.8.9-2017.8.16
1、We transform Rhodobacter sphaeroide by electric shock again, but the effect is not good.
2、We successfully constructed PIND4-SYFP2(unoptimized) plasmids.
3、Apply for ECUST-iGEM club
4、Cooperation with SUIS high school team
2017.8.16-2017.8.23
1、We transform Rhodobacter sphaeroide by electric shock, but the effect is not good.
2、Finished our first poster.
3、Cooperation with Tongji University.
2017.8.23-2017.8.30
Some of our team members participated in the CCiC meeting.
2017.8.30-2017.9.6
The SYFP2 expression strain was successfully obtained by electroporation.
2017.9.6-2017.9.13
We transform Rhodobacter sphaeroide by electric shock, but the effect is not good.
2017.9.13-2017.9.20
We interviewed Prof. Qingli Xu to understand the current energy crisis.
2017.9.20-2017.9.27
Completed the requirement for Part-submission and submitted 4 parts.
2017.9.27-2017.10.4
1、We made the PPT and rehearsed it.
2、Perfected our modeling.
2017.10.4-2017.10.11
1、Characterization of SYFP2 in cytoplasmic expression.
2、Editing Wiki pages.
3、Spectral scanning and fluorescence microscopy.
2017.10.11-2017.10.18
1、Perform the final fermentation hydrogen production experiment
2、Continue to Edit Wiki pages.
3、Assembling a luminous stirring oar and simulating the flow field.
4、Interviewed with Guangming Group.
5、Hold Fret-me Games.
2017.10.18-2017.10.25
1、Writing each copy of the wiki page.
2、Measuring photosynthetic growth curve.
3、Making new uniforms.
4、Editing Wiki
2017.10.25-2017.11.1
Optimized the final wiki page and the Registry page
