Difference between revisions of "Team:ECUST/Parts"

 
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            <th>Designer</th>
 
            <th>Designer</th>
 
            <th>Other</th>
 
            <th>Other</th>
−
            <th>Url</th>
 
−
            <th>img</th>
 
 
          </tr>
 
          </tr>
 
        </thead>
 
        </thead>
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          <tr>
 
          <tr>
 
            <td>1</td>
 
            <td>1</td>
−
<td>BBa_K2308001</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308001">BBa_K2308001</a></td>
 
<td>composite part</td>
 
<td>composite part</td>
 
<td>knock out (upstream1+downstream1)</td>
 
<td>knock out (upstream1+downstream1)</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td></td>
 
<td></td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308001">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/0/08/Newparts1.png" alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
 
          <tr>
 
          <tr>
 
            <td>2</td>
 
            <td>2</td>
−
<td>BBa_K2308002</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308002">BBa_K2308002</a></td>
 
<td>composite part</td>
 
<td>composite part</td>
 
<td>knock in (upstream2+sYFP2+downstream2)</td>
 
<td>knock in (upstream2+sYFP2+downstream2)</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td></td>
 
<td></td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308002">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/8/80/Newparts2.png" alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
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          <tr>
 
          <tr>
 
            <td>3</td>
 
            <td>3</td>
−
<td>BBa_K2308003</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308003">BBa_K2308003</a></td>
 
<td>basic part</td>
 
<td>basic part</td>
 
<td>sYFP2</td>
 
<td>sYFP2</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td>improvement of BBa_K864100 </td>
 
<td>improvement of BBa_K864100 </td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308003">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/0/0b/Parts6.png" alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
 
          <tr>
 
          <tr>
 
            <td>4</td>
 
            <td>4</td>
−
<td>BBa_K2308004</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308004">BBa_K2308004</a></td>
 
<td>composite part</td>
 
<td>composite part</td>
 
<td>knock out (upstream1+promotor+Amp+downstream1)</td>
 
<td>knock out (upstream1+promotor+Amp+downstream1)</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td></td>
 
<td></td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308004">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/b/bd/Newparts4.png" alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
 
          <tr>
 
          <tr>
 
            <td>5</td>
 
            <td>5</td>
−
<td>BBa_K2308005</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308005">BBa_K2308005</a></td>
 
<td>composite part</td>
 
<td>composite part</td>
 
<td>knock in (upstream2+sYFP2+promotor+Amp+downstream2)</td>
 
<td>knock in (upstream2+sYFP2+promotor+Amp+downstream2)</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td></td>
 
<td></td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308005">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/1/17/Newparts5.png" alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
 
          <tr>
 
          <tr>
 
            <td>6</td>
 
            <td>6</td>
−
<td>BBa_K2308006</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308006">BBa_K2308006</a></td>
 
<td>basic part</td>
 
<td>basic part</td>
 
<td>upstream1</td>
 
<td>upstream1</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td></td>
 
<td></td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308006">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/0/0b/Parts6.png"  alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
 
          <tr>
 
          <tr>
 
            <td>7</td>
 
            <td>7</td>
−
<td>BBa_K2308007</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308007">BBa_K2308007</a></td>
 
<td>basic part</td>
 
<td>basic part</td>
 
<td>downstream1</td>
 
<td>downstream1</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td></td>
 
<td></td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308007">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/0/0b/Parts6.png"  alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
 
          <tr>
 
          <tr>
 
            <td>8</td>
 
            <td>8</td>
−
<td>BBa_K2308008</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308008">BBa_K2308008</a></td>
 
<td>basic part</td>
 
<td>basic part</td>
 
<td>upstream2</td>
 
<td>upstream2</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td></td>
 
<td></td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308008">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/0/0b/Parts6.png"  alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
 
          <tr>
 
          <tr>
 
            <td>9</td>
 
            <td>9</td>
−
<td>BBa_K2308009</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308009">BBa_K2308009</a></td>
 
<td>basic part</td>
 
<td>basic part</td>
 
<td>downstream2</td>
 
<td>downstream2</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td></td>
 
<td></td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308009">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/0/0b/Parts6.png"  alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
 
          <tr>
 
          <tr>
 
            <td>10</td>
 
            <td>10</td>
−
<td>BBa_K2308010</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308010">BBa_K2308010</a></td>
 
<td>basic part</td>
 
<td>basic part</td>
 
<td>Amp promotor</td>
 
<td>Amp promotor</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td></td>
 
<td></td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308010">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/0/0b/Parts6.png"  alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
 
          <tr>
 
          <tr>
 
            <td>11</td>
 
            <td>11</td>
−
<td>BBa_K2308011</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308011">BBa_K2308011</a></td>
 
<td>basic part</td>
 
<td>basic part</td>
 
<td>Amp coding</td>
 
<td>Amp coding</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td></td>
 
<td></td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308011">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/0/0b/Parts6.png"  alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
 
          <tr>
 
          <tr>
 
            <td>12</td>
 
            <td>12</td>
−
<td>BBa_K2308012</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308012">BBa_K2308012</a></td>
 
<td>basic part</td>
 
<td>basic part</td>
 
<td>LacIq repressor</td>
 
<td>LacIq repressor</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td></td>
 
<td></td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308012">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/0/0b/Parts6.png"  alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
 
          <tr>
 
          <tr>
 
            <td>13</td>
 
            <td>13</td>
−
<td>BBa_K2308013</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308013">BBa_K2308013</a></td>
 
<td>basic part</td>
 
<td>basic part</td>
 
<td>LacIq spacer</td>
 
<td>LacIq spacer</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td></td>
 
<td></td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308013">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/0/0b/Parts6.png"  alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
 
          <tr>
 
          <tr>
 
            <td>14</td>
 
            <td>14</td>
−
<td>BBa_K2308014</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308014">BBa_K2308014</a></td>
 
<td>basic part</td>
 
<td>basic part</td>
 
<td>LacIq promotor</td>
 
<td>LacIq promotor</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td></td>
 
<td></td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308014">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/0/0b/Parts6.png"  alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
 
          <tr>
 
          <tr>
 
            <td>15</td>
 
            <td>15</td>
−
<td>BBa_K2308015</td>
+
<td> <a href="http://parts.igem.org/Part:BBa_K2308015">BBa_K2308015</a></td>
 
<td>basic part</td>
 
<td>basic part</td>
 
<td>LacIq coding</td>
 
<td>LacIq coding</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td></td>
 
<td></td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308015">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/0/0b/Parts6.png"  alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
 
  <tr>
 
  <tr>
 
            <td>16</td>
 
            <td>16</td>
−
<td>BBa_K2308016</td>
+
<td><a href="http://parts.igem.org/Part:BBa_K2308016">BBa_K2308016</a></td>
 
<td>composite part</td>
 
<td>composite part</td>
 
<td>LacIq regulator</td>
 
<td>LacIq regulator</td>
 
<td>Yunpeng Dai</td>
 
<td>Yunpeng Dai</td>
 
<td>improvement of BBa_v1003 </td>
 
<td>improvement of BBa_v1003 </td>
−
<td><a href="http://parts.igem.org/Part:BBa_K2308016">Click</a></td>
 
−
<td><img src="https://static.igem.org/mediawiki/2017/0/0b/Parts6.png"  alt="" style="width: 120px;height: 40px;"></td>
 
 
          </tr>
 
          </tr>
  
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<br><h1>Improvements</h1>
 
<br><h1>Improvements</h1>
 
<div class="page-header">
 
<div class="page-header">
−
<h2 id="tables">Improvement of Part:BBa K864100</h2>
+
<h2 id="tables">Improvement of <a href="http://parts.igem.org/Part:BBa_K864100">Part:BBa K864100</a></h2>
 
</div>
 
</div>
 
<div class="style1">
 
<div class="style1">
−
<p>The reporter sYPF2 has been codon-optimized for Rhodobacter sphaeroides 2.4.1 and it was successfully tested as a reporter in this chassis. We have made several measurements to show its fluorescence in Rhodobacter sphaeroides as well its properties (Fluorescence excitation/emission spectrum). For more information of the improved part, please go to the page of Part:BBa_K2136003.</p>
+
<p>The reporter sYPF2 has been codon-optimized for <i>Rhodobacter sphaeroides 2.4.1</i> and it was successfully tested as a reporter in this chassis. We have made several measurements to show its fluorescence in <i>Rhodobacter sphaeroides 2.4.1</i> as well its properties (Fluorescence excitation/emission spectrum).</p>
 
</div>
 
</div>
 +
<p><i> To learn more about the improvement ，<a href="http://parts.igem.org/Part:BBa_K2308003">please click here.</a></i></p>
 
<div class="some-padding"></div>
 
<div class="some-padding"></div>
 
<div class="some-padding"></div>
 
<div class="some-padding"></div>
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<div class="page-header">
 
<div class="page-header">
−
<h2 id="tables">Improvement of Part:BBa V1003</h2>
+
<h2 id="tables">Improvement of <a href="http://parts.igem.org/Part:BBa V1003">Part:BBa V1003</a></h2>
 
</div>
 
</div>
 
<div class="style1">
 
<div class="style1">
−
<p>The regulator LacIq's coding sequences are submitted. In our project, we used this part with Part:BBa_K2308003 together to turn Rhodobacter sphaeroides 2.4.1 into inducible strains and successfully induced the expression of Part_K2308003. For more information of the improved part, please go to the page of Part:BBa_K2136016.</p>
+
<p>The regulator LacIq's coding sequences are submitted in part BBa_K2308016. In our project, we used this part with Part:BBa_K2308003 together to turn <i>Rhodobacter sphaeroides 2.4.1</i> into inducible strains and successfully induced the expression of sYFP2. </p>
 +
 
</div>
 
</div>
 +
<p><i> To learn more about the improvement ，<a href="http://parts.igem.org/Part:BBa_K2308016">please click here.</a></i></p>
 
</div>
 
</div>
  
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<div class="style1">
 
<div class="style1">
 
<p>sYFP2(BBa_K2308003) is the key part in our experiment. It is used both for fusion expression (see BBa_K2308002) and inducible cytoplasm expression (combined with BBa_K2308016), and the host cell is <i>Rhodobacter sphaeroides 2.4.1.</i> </p><br>
 
<p>sYFP2(BBa_K2308003) is the key part in our experiment. It is used both for fusion expression (see BBa_K2308002) and inducible cytoplasm expression (combined with BBa_K2308016), and the host cell is <i>Rhodobacter sphaeroides 2.4.1.</i> </p><br>
−
<p>In inducible cytoplasm expression experiment, we used part BBa_K2308016 to turn the <i>Rhodobacter sphaedoides 2.4.1</i>  into inducible strains, and IPTG was added （800 μM in final volume）when the OD700 of the strain was about 0.4（grown for about 24h）.</p><br><br>
+
<p>In inducible cytoplasm expression experiment, we used part BBa_K2308016 to turn the <i>Rhodobacter sphaedoides 2.4.1</i>  into inducible strains, and IPTG was added （800 μM in final volume）when the OD<sub>700</sub> of the strain was about 0.4（grown for about 24h）.</p><br><br>
 
 
 
</div>
 
</div>
−
<p><i>To learn more about this part ，<a href="http://parts.igem.org/Part:BBa_K2308003"> please click here.</a></i></p>
+
<p><i>To learn more about this part ，<a href="http://parts.igem.org/Part:BBa_K2308003"> please click here.</a></i></p><br><br>
 
 
 
 
  
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</div>
 
</div>
 
<div class="style1">
 
<div class="style1">
−
<p> In inducible cytoplasm expression experiment, we used part BBa_K2308016 to turn the Rhodobacter sphaedoides 2.4.1 into inducible strains, and IPTG was added （800 μM in final volume）when the OD700 of the strain was about 0.4（grown for about 24h）.</p><br><br>
+
<p>This part contains LacIq repressor, LacIq spacer, LacIq promotor and LacIq coding sequence and is used for <i>Rhodobacter sphaeroides 2.4.1</i>, cells with the device can carry out inducible expression of the target proteins. </p><br>
 +
                                        <p>In inducible cytoplasm expression experiment, we used part BBa_K2308016 to turn the <i>Rhodobacter sphaeroides 2.4.1</i> into inducible strains, and IPTG was added （800 μM in final volume）when the OD<sub>700</sub> of the strain was about 0.4（grown for about 24h）.</p><br><br>
 
 
 
</div>
 
</div>
−
<p><i> To learn more about this part ，<a href="http://parts.igem.org/Part:BBa_K2308016">please click here.</a></i></p>
+
<p><i> To learn more about this part ，<a href="http://parts.igem.org/Part:BBa_K2308016">please click here.</a></i></p><br><br>
 
 
 
 
  
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</html>
 
</html>
  
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{{:Team:ECUST/footer}}
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<p>BBa_K2308002 is an important part in our experiment. It is composed of BBa_K2308006，BBa_K2308003 and BBa_K2308007. The function of this part is to carry out the homologous recombination on the genome of <i>Rhodobacter sphaeroides 2.4.1.</i></p><br><br>
+
−
 
+
−
<p>In the experiment, sYFP2 was designed to be fused to the C-terminal of subunit H in the reaction center(RC) of <i>Rhodobacter sphaeroides 2.4.1.</i> </p>
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<img src="https://static.igem.org/mediawiki/2017/5/59/Fusion_protein.gif" alt="">
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<center><p style="font-size: 10px;">Figure 1：Modelling of RC-sYFP2 (GIF)</p></center>
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<p>In fusion expression experiment: we constructed the plasmid using part BBa_K2308002, and after transformation and gene recombination, single clones are selected by antibiotics and PCR tests. </p>
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<img src="https://static.igem.org/mediawiki/2017/0/04/F1-B.jpg" alt="">
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<center><p style="font-size: 10px;">Figure 2：PCR test of BBa_K2308002</p></center>
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<p>Fluorescence image shows sYFP2 which is fused with H submit of <i>Rhodobacter sphaeroides 2.4.1</i> ’s reaction center folded correctly and was able to emit fluorescence. </p>
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<img src="https://static.igem.org/mediawiki/2017/b/b9/F3.jpg" alt="">
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<center><p style="font-size: 10px;">Figure 3：Fluorescence image of whole cells of RC-SYFP2 cells when excited at 495nm</p></center>
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<img src="https://static.igem.org/mediawiki/2017/3/34/F2.png" alt="">
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<center><p style="font-size: 10px;">Figure 4：Absorption spectra of RC-sYFP2 strains compared with wild type.</p></center>
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<p> The RC-sYFP2 fusion strain was created in a wild type background. Absorption spectrum of membranes from this strain recorded at room temperature show no isolated sYFP2 peak due to overlap with absorption of carotenoid B at 514 nm.</p>
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<img src="https://static.igem.org/mediawiki/2017/4/42/F4.png" alt="">
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<center><p style="font-size: 10px;">Figure 5：Growth rate of WT and WT RC-sYFP2.</p></center>
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<p>The biomass of Rhodobacter sphaeroides 2.4.1 didn’t arise very much since the energy from sYFP2 is too little.</p>
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Latest revision as of 03:33, 2 November 2017



New Parts

# Name Type Description Designer Other
1 BBa_K2308001 composite part knock out (upstream1+downstream1) Yunpeng Dai
2 BBa_K2308002 composite part knock in (upstream2+sYFP2+downstream2) Yunpeng Dai
3 BBa_K2308003 basic part sYFP2 Yunpeng Dai improvement of BBa_K864100
4 BBa_K2308004 composite part knock out (upstream1+promotor+Amp+downstream1) Yunpeng Dai
5 BBa_K2308005 composite part knock in (upstream2+sYFP2+promotor+Amp+downstream2) Yunpeng Dai
6 BBa_K2308006 basic part upstream1 Yunpeng Dai
7 BBa_K2308007 basic part downstream1 Yunpeng Dai
8 BBa_K2308008 basic part upstream2 Yunpeng Dai
9 BBa_K2308009 basic part downstream2 Yunpeng Dai
10 BBa_K2308010 basic part Amp promotor Yunpeng Dai
11 BBa_K2308011 basic part Amp coding Yunpeng Dai
12 BBa_K2308012 basic part LacIq repressor Yunpeng Dai
13 BBa_K2308013 basic part LacIq spacer Yunpeng Dai
14 BBa_K2308014 basic part LacIq promotor Yunpeng Dai
15 BBa_K2308015 basic part LacIq coding Yunpeng Dai
16 BBa_K2308016 composite part LacIq regulator Yunpeng Dai improvement of BBa_v1003

Improvements

Improvement of Part:BBa K864100

The reporter sYPF2 has been codon-optimized for Rhodobacter sphaeroides 2.4.1 and it was successfully tested as a reporter in this chassis. We have made several measurements to show its fluorescence in Rhodobacter sphaeroides 2.4.1 as well its properties (Fluorescence excitation/emission spectrum).

To learn more about the improvement ，please click here.

Improvement of Part:BBa V1003

The regulator LacIq's coding sequences are submitted in part BBa_K2308016. In our project, we used this part with Part:BBa_K2308003 together to turn Rhodobacter sphaeroides 2.4.1 into inducible strains and successfully induced the expression of sYFP2.

To learn more about the improvement ，please click here.


Key parts

BBa_K2308002

BBa_K2308002 is an important part in our experiment. It is composed of BBa_K2308006，BBa_K2308003 and BBa_K2308007. The function of this part is to carry out the homologous recombination on the genome of Rhodobacter sphaeroides 2.4.1.



To learn more about this part ，please click here.



BBa_K2308003

sYFP2(BBa_K2308003) is the key part in our experiment. It is used both for fusion expression (see BBa_K2308002) and inducible cytoplasm expression (combined with BBa_K2308016), and the host cell is Rhodobacter sphaeroides 2.4.1.


In inducible cytoplasm expression experiment, we used part BBa_K2308016 to turn the Rhodobacter sphaedoides 2.4.1 into inducible strains, and IPTG was added （800 μM in final volume）when the OD700 of the strain was about 0.4（grown for about 24h）.



To learn more about this part ， please click here.



BBa_K2308016

This part contains LacIq repressor, LacIq spacer, LacIq promotor and LacIq coding sequence and is used for Rhodobacter sphaeroides 2.4.1, cells with the device can carry out inducible expression of the target proteins.


In inducible cytoplasm expression experiment, we used part BBa_K2308016 to turn the Rhodobacter sphaeroides 2.4.1 into inducible strains, and IPTG was added （800 μM in final volume）when the OD700 of the strain was about 0.4（grown for about 24h）.



To learn more about this part ，please click here.