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location listed in figure 1). We were asked to measure the fluorescent output of these samples using equipment | location listed in figure 1). We were asked to measure the fluorescent output of these samples using equipment | ||
in our lab.</h4> | in our lab.</h4> | ||
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− | <img src="https://static.igem.org/mediawiki/2017/5/54/T--CPU_CHINA--interlab_fig1.jpg | + | <center><img src="https://static.igem.org/mediawiki/2017/5/54/T--CPU_CHINA--interlab_fig1.jpg" width="900"></center> |
<h4 align=middle>Figure 1. The locations of devices</h4> | <h4 align=middle>Figure 1. The locations of devices</h4> | ||
</div> | </div> | ||
<h4><br>The CPU_CHINA team measured these samples on our Multimode Reader and reported the results to iGEM HQ for further | <h4><br>The CPU_CHINA team measured these samples on our Multimode Reader and reported the results to iGEM HQ for further | ||
analysis and comparison to other teams' results before deadline(figure 2).</h4> | analysis and comparison to other teams' results before deadline(figure 2).</h4> | ||
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− | <img src="https://static.igem.org/mediawiki/2017/3/32/T--CPU_CHINA--interlab_fig2.png" | + | <center><img src="https://static.igem.org/mediawiki/2017/3/32/T--CPU_CHINA--interlab_fig2.png" wdith = "900"></center> |
<h4 align=middle>Figure 2. The iGEMer is measuring these devices</h4> | <h4 align=middle>Figure 2. The iGEMer is measuring these devices</h4> | ||
</div> | </div> | ||
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<br> Import data into Excel (fluorescein standard curve tab ) Sheet_1 provided. | <br> Import data into Excel (fluorescein standard curve tab ) Sheet_1 provided. | ||
</h4> | </h4> | ||
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− | <img src="https://static.igem.org/mediawiki/2017/8/8a/T--CPU_CHINA--interlab_fig3.jpg" | + | <center><img src="https://static.igem.org/mediawiki/2017/8/8a/T--CPU_CHINA--interlab_fig3.jpg" width="700"></center> |
<h4 align=middle>Figure 3. Overview of 96 well plate setup</h4> | <h4 align=middle>Figure 3. Overview of 96 well plate setup</h4> | ||
</div> | </div> | ||
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<h4><br>Notes: The measurement protocol takes kit plate 6 as an example. It is the same protocol, when measuring the kit | <h4><br>Notes: The measurement protocol takes kit plate 6 as an example. It is the same protocol, when measuring the kit | ||
plate 7.</h4> | plate 7.</h4> | ||
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− | <img src="https://static.igem.org/mediawiki/2017/6/6e/T--CPU_CHINA--interlab_fig4.png" | + | <center><img src="https://static.igem.org/mediawiki/2017/6/6e/T--CPU_CHINA--interlab_fig4.png" width="700"></center> |
<h4 align=middle>Figure 4. Overview of the Cell measurement protocol</h4> | <h4 align=middle>Figure 4. Overview of the Cell measurement protocol</h4> | ||
</div> | </div> | ||
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<br>● Export data on the desktop. | <br>● Export data on the desktop. | ||
<br>● Import data into Excel ( cell measurement tab ) Sheet_1 provided.</h4> | <br>● Import data into Excel ( cell measurement tab ) Sheet_1 provided.</h4> | ||
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− | <img src="https://static.igem.org/mediawiki/2017/3/3f/T--CPU_CHINA--interlab_fig5.jpg" | + | <center><img src="https://static.igem.org/mediawiki/2017/3/3f/T--CPU_CHINA--interlab_fig5.jpg" width="900"></center> |
<h4 align=middle>Figure 5. Overview of the samples’ locations in 96 well plate for each time point</h4> | <h4 align=middle>Figure 5. Overview of the samples’ locations in 96 well plate for each time point</h4> | ||
</div> | </div> | ||
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<h4><br>We used LUDOX-S40 as a single point reference to obtain a ratiometric conversion factor and transform our absorbance | <h4><br>We used LUDOX-S40 as a single point reference to obtain a ratiometric conversion factor and transform our absorbance | ||
data into the standard OD 600 measurement (table6).</h4> | data into the standard OD 600 measurement (table6).</h4> | ||
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− | <img src="https://static.igem.org/mediawiki/2017/b/bc/T--CPU_CHINA--interlab_fig6.png" alt=""> | + | <center><img src="https://static.igem.org/mediawiki/2017/b/bc/T--CPU_CHINA--interlab_fig6.png" alt=""></center> |
<h4 align=middle>Table 1. The data of OD600 measurement</h4> | <h4 align=middle>Table 1. The data of OD600 measurement</h4> | ||
</div> | </div> | ||
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plate reader. By measuring these in all standard modes in the plate reader, We got a standard curve of fluorescence | plate reader. By measuring these in all standard modes in the plate reader, We got a standard curve of fluorescence | ||
for fluorescein concentration (table 2 and 3).</h4> | for fluorescein concentration (table 2 and 3).</h4> | ||
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− | <img src="https://static.igem.org/mediawiki/2017/6/63/T--CPU_CHINA--interlab_fig7.jpg" | + | <center><img src="https://static.igem.org/mediawiki/2017/6/63/T--CPU_CHINA--interlab_fig7.jpg" width = "700"></center> |
<h4 align=middle>Table 2. The data of fluorescence measurement</h4> | <h4 align=middle>Table 2. The data of fluorescence measurement</h4> | ||
</div> | </div> | ||
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− | <img src="https://static.igem.org/mediawiki/2017/a/af/T--CPU_CHINA--interlab_fig8.jpg" | + | <center><img src="https://static.igem.org/mediawiki/2017/a/af/T--CPU_CHINA--interlab_fig8.jpg" width="700"></center> |
<h4 align=middle>Table 3. The fluorescence standard curve</h4> | <h4 align=middle>Table 3. The fluorescence standard curve</h4> | ||
</div> | </div> | ||
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<h4><br>Hereafter we measured the Abs600 and fluorescence of all of the cell samples we took. We measured the fluorescence | <h4><br>Hereafter we measured the Abs600 and fluorescence of all of the cell samples we took. We measured the fluorescence | ||
with the same settings as the standard modes( table4 and 5 ).</h4> | with the same settings as the standard modes( table4 and 5 ).</h4> | ||
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− | <img src="https://static.igem.org/mediawiki/2017/6/63/T--CPU_CHINA--interlab_fig9.jpg" | + | <center><img src="https://static.igem.org/mediawiki/2017/6/63/T--CPU_CHINA--interlab_fig9.jpg" width="700"></center> |
<h4 align=middle>Table 4. The fluorescence of all of the cell samples(kit plate 6)</h4> | <h4 align=middle>Table 4. The fluorescence of all of the cell samples(kit plate 6)</h4> | ||
</div> | </div> | ||
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− | <img src="https://static.igem.org/mediawiki/2017/4/4d/T--CPU_CHINA--interlab_fig10.jpg" | + | <center><img src="https://static.igem.org/mediawiki/2017/4/4d/T--CPU_CHINA--interlab_fig10.jpg" width="700"></center> |
<h4 align=middle>Table 5. The fluorescence and OD600 of all of the cell samples at 6 h(kit plate 6)</h4> | <h4 align=middle>Table 5. The fluorescence and OD600 of all of the cell samples at 6 h(kit plate 6)</h4> | ||
</div> | </div> | ||
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/ 6 | / 6 | ||
</h4> | </h4> | ||
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− | <img src="https://static.igem.org/mediawiki/2017/5/55/T--CPU_CHINA--interlab_fig11.jpg" | + | <center><img src="https://static.igem.org/mediawiki/2017/5/55/T--CPU_CHINA--interlab_fig11.jpg" width="700"></center> |
<h4 align=middle>Table 6. The fluorescein/OD600 of samples at 6 h(kit plate 6)</h4> | <h4 align=middle>Table 6. The fluorescein/OD600 of samples at 6 h(kit plate 6)</h4> | ||
</div> | </div> | ||
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− | <img src="https://static.igem.org/mediawiki/2017/d/d3/T--CPU_CHINA--interlab_fig12.jpg" | + | <center><img src="https://static.igem.org/mediawiki/2017/d/d3/T--CPU_CHINA--interlab_fig12.jpg" width="700"></center> |
<h4 align=middle>Table 7. The fluorescein/OD600 of samples at 6 h(kit plate 7)</h4> | <h4 align=middle>Table 7. The fluorescein/OD600 of samples at 6 h(kit plate 7)</h4> | ||
</div> | </div> |
Revision as of 13:34, 31 October 2017