Difference between revisions of "Team:CPU CHINA/collaborations"

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         <h4><br>According to the figure, we confirmed that the electroporation was successful and thus we were able to continue our experiments. We would like to thank the NJU-CHINA team for the great help for our experiments and for the provision of IL-17RA antibodies.</h4>
 
         <h4><br>According to the figure, we confirmed that the electroporation was successful and thus we were able to continue our experiments. We would like to thank the NJU-CHINA team for the great help for our experiments and for the provision of IL-17RA antibodies.</h4>
 
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             <img src="https://static.igem.org/mediawiki/2017/3/31/T--CPU_CHINA--collaborations_fig.png" width = "700">
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             <center><img src="https://static.igem.org/mediawiki/2017/3/31/T--CPU_CHINA--collaborations_fig.png" width = "700"></center>
  
 
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Revision as of 14:52, 31 October 2017

Collaborations

Collaboration with NJU-CHINA


Our team helped the NJU_CHINA team design two sequence of BCL2-siRNA.


The sequences are as follows:


BCL2-siRNA-3 sequence: GTGGATGACTGAGTACCTGAA
BCL2-siRNA-4 sequence: CTGCATCACTCTGGGTGCATA


The NJU_CHINA team helped us to detect the expression level of Myc-CAR-CD20 and IL-17RA-SynNotch with Western blot. Flag-FOXP3-Jurkat cells expressed the proteins after transfection by electroporation. The result is shown in the figure. They also provided us with human IL-17RA antibodies.


According to the figure, we confirmed that the electroporation was successful and thus we were able to continue our experiments. We would like to thank the NJU-CHINA team for the great help for our experiments and for the provision of IL-17RA antibodies.