Difference between revisions of "Team:UChile OpenBio-CeBiB/Model"

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<ul>
 
<ul>
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<li id="liOv">Elaborate a kinetic model to prove that the insertion of FBP/SBPase gene increases carbon
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<li id="liOv">Elaborate a kinetic model to describe the actions of inserting FBP/SBPase regarding carbon
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dioxide fixation and quantify the amount.</li>
+
dioxide fixation.</li>
  
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<li id="liOv">Construct a kinetic model of the ideas that we won’t be able to verify experimentally, these are
+
<li id="liOv">Construct a kinetic model of the ideas that we won’t be able to verify experimentally in this first approach, this is the regulation of starch synthesis pathways by the means of metE operator sensible to vitamin
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the regulation of starch synthesis pathways by the means of metE operator sensible to vitamin
+
 
B12. This would allow the usage of glucose for other economical purposes, and it the preferred
 
B12. This would allow the usage of glucose for other economical purposes, and it the preferred
−
method is through the use of antisense RNA. A comparison between the B12-sensible promoter
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method is through the use of antisense RNA.</li>
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and a light-induceable one will also be studied.</li>
+
 
 +
<li id="liOv>Evaluate the parameters by sensitivity analysis, in order to select which of them are susceptible to optimization, this way it is possible to know whether B12 promoter will actually do the trick, or if a different promoter would be even better. Moreover, we can know which reactions of the model could be further studied to propose new parts.
  
 
<li id="liOv">Get an estimation or quantify the production of certain substances that could be generated in
 
<li id="liOv">Get an estimation or quantify the production of certain substances that could be generated in

Revision as of 21:04, 1 November 2017

Document

Modellling


Modelling commission has the objective of giving information that contributes to implementation and designing of our project through mathematical representation of the metabolic pathways involved and genetic expression kinetics. To achieve this end, the following proposals will be accomplished:

  • Elaborate a kinetic model to describe the actions of inserting FBP/SBPase regarding carbon dioxide fixation.
  • Construct a kinetic model of the ideas that we won’t be able to verify experimentally in this first approach, this is the regulation of starch synthesis pathways by the means of metE operator sensible to vitamin B12. This would allow the usage of glucose for other economical purposes, and it the preferred method is through the use of antisense RNA.
  • Get an estimation or quantify the production of certain substances that could be generated in our microalgae by this genetic circuit.
  • Carry out an economic analysis that could help evaluate the viability of the usage of this modified microalgae in bioreactors.