Difference between revisions of "Team:UChile OpenBio-CeBiB/Model"
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| − | < | + | <cite>Genetic and metabolic pathways of our model. The Calcin Cycle evaluated is a simplified version.</cite> |
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| + | <cite>Antisense mechanism. Transgene transcription is inhibited by presence of vitamin B12. In absence of B12, | ||
| + | the wild-type RNA is sequestrated by the corresponding antisense RNA, inhibitng starch synthesis.</cite> | ||
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Revision as of 00:50, 2 November 2017
Modelling Overview
Modelling commission has the objective of giving information that contributes to implementation and designing of our project through mathematical representation of the metabolic pathways involved and genetic expression kinetics. To achieve this end, the following proposals will be accomplished:
- Elaborate a kinetic model to describe the actions of inserting FBP/SBPase regarding carbon dioxide fixation.
- Construct a kinetic model of the ideas that we won’t be able to verify experimentally in this first approach, this is the regulation of starch synthesis pathways by the means of metE operator sensible to vitamin B12. This would allow the usage of glucose for other economical purposes, and it the preferred method is through the use of antisense RNA.
- Get an estimation or quantify the production of certain substances that could be generated in our microalgae by this genetic circuit.
- Carry out an economic analysis that could help evaluate the viability of the usage of this modified microalgae in bioreactors.
