Difference between revisions of "Team:Edinburgh OG"

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                                 <span>Antibiotic revolution: a molecular toolkit to re-sensitise </span><br>
 
                                 <span>Antibiotic revolution: a molecular toolkit to re-sensitise </span><br>
 
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<h2 class="wow fadeInUp animated" data-wow-delay=".6s">
 
                                     Modular molecular toolkit for re-sensitisation of antibiotic-resistant pathogens using CRISPR delivered by a two-phage system.
 
                                     Modular molecular toolkit for re-sensitisation of antibiotic-resistant pathogens using CRISPR delivered by a two-phage system.
 
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          <a class="btn-lines dark light wow fadeInUp animated smooth-scroll btn btn-default btn-green" data-wow-delay=".9s" href="#works" data-section="#about">Read more</a>
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<a class="btn-lines dark light wow fadeInUp animated smooth-scroll btn btn-default btn-green" data-wow-delay=".9s" href="#works" data-section="#about">Read more</a>
  
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                                 ABOUT PhagED
 
                                 ABOUT PhagED
 
                                 </h2>
 
                                 </h2>
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            The threat posed by antibiotic resistant bacteria is a pressing issue which must be addressed. It is difficult and expensive to develop new antibiotics, so our project is designed to make currently available antibiotics useful again. Our aim is to create a toolkit to re-sensitise pathogens to antibiotics using CRISPR and a two-phage system, based on work by <b style="font-weight: bold;">Yosef et al. (2015, doi:10.1073/pnas.1500107112)</b>. An engineered lysogenic phage will transfer a CRISPR system to its host bacterium, designed to cleave resistance genes and also confer protection from an engineered lytic phage. When this lytic phage is added to the population, it kills any bacteria that have not been successfully re-sensitised. We chose to target genes found in the highly-resistant ESKAPE pathogens, and worked with 4 different phages - P1, lambda, T4 and T7. Our system was modelled in silico and tested empirically on a specially designed E. coli testing platform.
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The threat posed by antibiotic resistant bacteria is a pressing issue which must be addressed. It is difficult and expensive to develop new antibiotics, so our project is designed to make currently available antibiotics useful again. Our aim is to create
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          </p>
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a toolkit to re-sensitise pathogens to antibiotics using CRISPR and a two-phage system, based on work by <b style="font-weight: bold;">Yosef et al. (2015, doi:10.1073/pnas.1500107112)</b>. An engineered lysogenic phage will transfer a CRISPR
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          <p>
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system to its host bacterium, designed to cleave resistance genes and also confer protection from an engineered lytic phage. When this lytic phage is added to the population, it kills any bacteria that have not been successfully re-sensitised. We
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          Figure: Yosef et al., 2015
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chose to target genes found in the highly-resistant ESKAPE pathogens, and worked with 4 different phages - P1, lambda, T4 and T7. Our system was modelled in silico and tested empirically on a specially designed E. coli testing platform.
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Figure: Yosef et al., 2015
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          <h1 class="title wow fadeInDown" data-wow-delay=".3s">TAKE A CLOSER LOOK</h1>
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<h1 class="title wow fadeInDown" data-wow-delay=".3s">TAKE A CLOSER LOOK</h1>
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Aliquam lobortis. Maecenas vestibulum mollis diam. Pellentesque auctor neque nec urna. Nulla sit amet est. Aenean posuere <br> tortor sed cursus feugiat, nunc augue blandit nunc, eu sollicitudin urna dolor sagittis lacus.
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<img src="https://static.igem.org/mediawiki/2017/e/ea/T--Edinburgh_OG--item-1.png" class="img-responsive" alt="this is a title">
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                    <a target="_blank" href="https://2017.igem.org/Team:Edinburgh_OG/Description">Details</a>
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<a target="_blank" href="https://2017.igem.org/Team:Edinburgh_OG/Description">Details</a>
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                                 <a href="https://2017.igem.org/Team:Edinburgh_OG/Description">
 
                                 <a href="https://2017.igem.org/Team:Edinburgh_OG/Description">
 
                                     Project
 
                                     Project
 
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<p>
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                  Get to know PhagED better.
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Get to know PhagED better.
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<img src="https://static.igem.org/mediawiki/2017/9/96/T--Edinburgh_OG--item-2.png" class="img-responsive" alt="this is a title">
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                    <a target="_blank" href="https://2017.igem.org/Team:Edinburgh_OG/Parts">Details</a>
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                                 <a href="https://2017.igem.org/Team:Edinburgh_OG/Parts">
 
                                 <a href="https://2017.igem.org/Team:Edinburgh_OG/Parts">
 
                                     Parts
 
                                     Parts
 
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                  Blocks of DNA that we've used.
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Blocks of DNA that we've used.
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                    <a target="_blank" href="https://2017.igem.org/Team:Edinburgh_OG/Safety">Details</a>
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                                 <a href="https://2017.igem.org/Team:Edinburgh_OG/Safety">
 
                                 <a href="https://2017.igem.org/Team:Edinburgh_OG/Safety">
 
                                     Safety
 
                                     Safety
 
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                                 </a>
 
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                  Safety always comes first.
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Safety always comes first.
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</p>
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                    <a target="_blank" href="https://2017.igem.org/Team:Edinburgh_OG/HP/Silver">Details</a>
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                                 <a href="https://2017.igem.org/Team:Edinburgh_OG/HP/Silver">
 
                                 <a href="https://2017.igem.org/Team:Edinburgh_OG/HP/Silver">
 
                                     Human Practices
 
                                     Human Practices
 
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                  How does PhagED fit into the context of our lives?
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How does PhagED fit into the context of our lives?
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          <div class="media-body">
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            <h4 class="media-heading">Well documented.</h4>
+
<h4 class="media-heading">Well documented.</h4>
−
            <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Voluptatum, sint.</p>
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<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Voluptatum, sint.</p>
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        <div class="media wow fadeInDown animated" data-wow-duration="500ms" data-wow-delay="1200ms">
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              <i class="ion-ios-americanfootball-outline"></i>
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<i class="ion-ios-americanfootball-outline"></i>
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            </div>
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          <div class="media-body">
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            <h4 class="media-heading">Free updates</h4>
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<h4 class="media-heading">Free updates</h4>
−
            <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Voluptatum, sint.</p>
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<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Voluptatum, sint.</p>
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              <i class="ion-ios-keypad-outline"></i>
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          <div class="media-body">
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            <h4 class="media-heading">Solid Support</h4>
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<h4 class="media-heading">Solid Support</h4>
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            <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Voluptatum, sint.</p>
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</div>
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          <div class="media-body">
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            <h4 class="media-heading">Simple Installation</h4>
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<h4 class="media-heading">Simple Installation</h4>
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            <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Voluptatum, sint.</p>
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</section>
 
<!--
 
<!--
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      <h2 class="subtitle text-center">Meet The Team</h2>
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<h2 class="subtitle text-center">Meet The Team</h2>
−
      <div class="col-md-3">
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<div class="col-md-3">
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        <div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
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<div class="team-img">
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            <img src="https://static.igem.org/mediawiki/2017/e/e6/T--Edinburgh_OG--filippo.png" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/e/e6/T--Edinburgh_OG--filippo.png" class="team-pic" alt="">
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          </div>
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</div>
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          <h3 class="team_name">Filippo Abbondanza</h3>
+
<h3 class="team_name">Filippo Abbondanza</h3>
−
          <p class="team_designation">MSc Synthetic Biology & Biotechnology</p>
+
<p class="team_designation">MSc Synthetic Biology & Biotechnology</p>
−
          <p class="team_text">Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.</p>
+
<p class="team_text">Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.</p>
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          <p class="social-icons">
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<p class="social-icons">
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            <a href="https://www.facebook.com/filippo.abbondanza" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
+
<a href="https://www.facebook.com/filippo.abbondanza" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
−
            <a href="https://www.linkedin.com/in/filippo-abbondanza-a9130897" target="_blank"><i class="ion-social-linkedin-outline"></i></a>
+
<a href="https://www.linkedin.com/in/filippo-abbondanza-a9130897" target="_blank"><i class="ion-social-linkedin-outline"></i></a>
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          </p>
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</p>
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        </div>
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</div>
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      </div>
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</div>
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      <div class="col-md-3">
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<div class="col-md-3">
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        <div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".5s">
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<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".5s">
−
          <div class="team-img">
+
<div class="team-img">
−
            <img src="https://static.igem.org/mediawiki/2017/8/8e/T--Edinburgh_OG--erin.png" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/8/8e/T--Edinburgh_OG--erin.png" class="team-pic" alt="">
−
          </div>
+
</div>
−
          <h3 class="team_name">Erin Corbett</h3>
+
<h3 class="team_name">Erin Corbett</h3>
−
          <p class="team_designation">MSc Synthetic Biology & Biotechnology</p>
+
<p class="team_designation">MSc Synthetic Biology & Biotechnology</p>
−
          <p class="team_text">Engineering <i>E. coli</i> to create our mock pathogen testing platform, and engineering the lytic T7 phage.</p>
+
<p class="team_text">Engineering <i>E. coli</i> to create our mock pathogen testing platform, and engineering the lytic T7 phage.</p>
−
          <p class="social-icons">
+
<p class="social-icons">
−
          </p>
+
</p>
−
        </div>
+
</div>
−
      </div>
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</div>
−
      <div class="col-md-3">
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<div class="col-md-3">
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        <div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".7s">
+
<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".7s">
−
          <div class="team-img">
+
<div class="team-img">
−
            <img src="https://static.igem.org/mediawiki/2017/a/a5/T--Edinburgh_OG--rikki.jpg" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/a/a5/T--Edinburgh_OG--rikki.jpg" class="team-pic" alt="">
−
          </div>
+
</div>
−
          <h3 class="team_name">Yunqi He</h3>
+
<h3 class="team_name">Yunqi He</h3>
−
          <p class="team_designation">MSc Biochemistry</p>
+
<p class="team_designation">MSc Biochemistry</p>
−
          <p class="team_text">Engineering the lysogenic P1 phage with a CRISPR system to target resistance gene fragments.</p>
+
<p class="team_text">Engineering the lysogenic P1 phage with a CRISPR system to target resistance gene fragments.</p>
−
          <p class="social-icons">
+
<p class="social-icons">
−
            <a href="https://www.facebook.com/y.q.heybd" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
+
<a href="https://www.facebook.com/y.q.heybd" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
  
−
          </p>
+
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        </div>
+
</div>
−
      </div>
+
</div>
−
      <div class="col-md-3">
+
<div class="col-md-3">
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        <div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".9s">
+
<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".9s">
−
          <div class="team-img">
+
<div class="team-img">
−
            <img src="https://static.igem.org/mediawiki/2017/3/35/T--Edinburgh_OG--ti.jpg" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/3/35/T--Edinburgh_OG--ti.jpg" class="team-pic" alt="">
−
            <h3 class="team_name">Ti He</h3>
+
<h3 class="team_name">Ti He</h3>
−
            <p class="team_designation">MSc Biotechnology</p>
+
<p class="team_designation">MSc Biotechnology</p>
−
            <p class="team_text">Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.</p>
+
<p class="team_text">Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.</p>
−
            <p class="social-icons">
+
<p class="social-icons">
−
              <a href="https://www.facebook.com/profile.php?id=100012772305809" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
+
<a href="https://www.facebook.com/profile.php?id=100012772305809" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
−
            </p>
+
</p>
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          </div>
+
</div>
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        </div>
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</div>
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      </div>
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−
      <div class="row">
+
<div class="row">
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          <div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
+
<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
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            <div class="team-img">
+
<div class="team-img">
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              <img src="https://static.igem.org/mediawiki/2017/f/fa/T--Edinburgh_OG--lydia.jpg" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/f/fa/T--Edinburgh_OG--lydia.jpg" class="team-pic" alt="">
−
            </div>
+
</div>
−
            <h3 class="team_name">Lydia Mapstone</h3>
+
<h3 class="team_name">Lydia Mapstone</h3>
−
            <p class="team_designation">MSc Synthetic Biology & Biotechnology</p>
+
<p class="team_designation">MSc Synthetic Biology & Biotechnology</p>
−
            <p class="team_text">Engineering <i>E. coli</i> to create our mock pathogen testing platform and engineering the lytic T4 phage using BRED.</p>
+
<p class="team_text">Engineering <i>E. coli</i> to create our mock pathogen testing platform and engineering the lytic T4 phage using BRED.</p>
−
            <p class="social-icons">
+
<p class="social-icons">
−
            </p>
+
</p>
−
          </div>
+
</div>
−
        </div>
+
</div>
−
        <div class="col-md-3">
+
<div class="col-md-3">
−
          <div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".5s">
+
<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".5s">
−
            <div class="team-img">
+
<div class="team-img">
−
              <img src="https://static.igem.org/mediawiki/2017/2/27/T--Edinburgh_OG--yuri.jpg" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/2/27/T--Edinburgh_OG--yuri.jpg" class="team-pic" alt="">
−
            </div>
+
</div>
−
            <h3 class="team_name">Yuri Matsueda</h3>
+
<h3 class="team_name">Yuri Matsueda</h3>
−
            <p class="team_designation">MSc Biotechnology</p>
+
<p class="team_designation">MSc Biotechnology</p>
−
            <p class="team_text">Engineering the lysogenic P1 phage with a CRISPR system to target resistance gene fragments.</p>
+
<p class="team_text">Engineering the lysogenic P1 phage with a CRISPR system to target resistance gene fragments.</p>
−
            <p class="social-icons">
+
<p class="social-icons">
−
              <a href="https://www.facebook.com/profile.php?id=100004212933551" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
+
<a href="https://www.facebook.com/profile.php?id=100004212933551" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
−
            </p>
+
</p>
−
          </div>
+
</div>
−
        </div>
+
</div>
−
        <div class="col-md-3">
+
<div class="col-md-3">
−
          <div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".7s">
+
<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".7s">
−
            <div class="team-img">
+
<div class="team-img">
−
              <img src="https://static.igem.org/mediawiki/2017/d/d8/T--Edinburgh_OG--anton.jpg" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/d/d8/T--Edinburgh_OG--anton.jpg" class="team-pic" alt="">
−
            </div>
+
</div>
−
            <h3 class="team_name">Anton Puzorjov</h3>
+
<h3 class="team_name">Anton Puzorjov</h3>
−
            <p class="team_designation">MSc Bioinformatics</p>
+
<p class="team_designation">MSc Bioinformatics</p>
−
            <p class="team_text">Building a model of bacteria-phage interactions in two-step re-sensitisation combining both lysogenic and lytic phages.</p>
+
<p class="team_text">Building a model of bacteria-phage interactions in two-step re-sensitisation combining both lysogenic and lytic phages.</p>
−
            <p class="social-icons">
+
<p class="social-icons">
−
              <a href="https://www.facebook.com/puzorjov" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
+
<a href="https://www.facebook.com/puzorjov" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
−
              <a href="https://twitter.com/AntonPuzorjov" target="_blank"><i class="ion-social-twitter-outline"></i></a>
+
<a href="https://twitter.com/AntonPuzorjov" target="_blank"><i class="ion-social-twitter-outline"></i></a>
−
              <a href="https://www.linkedin.com/in/antonpuzorjov/" target="_blank"><i class="ion-social-linkedin-outline"></i></a>
+
<a href="https://www.linkedin.com/in/antonpuzorjov/" target="_blank"><i class="ion-social-linkedin-outline"></i></a>
−
            </p>
+
</p>
−
          </div>
+
</div>
−
        </div>
+
</div>
−
        <div class="col-md-3">
+
<div class="col-md-3">
−
          <div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".9s">
+
<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".9s">
−
            <div class="team-img">
+
<div class="team-img">
−
              <img src="https://static.igem.org/mediawiki/2017/7/70/T--Edinburgh_OG--yating.jpg" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/7/70/T--Edinburgh_OG--yating.jpg" class="team-pic" alt="">
−
            </div>
+
</div>
−
            <h3 class="team_name">Yating Wang</h3>
+
<h3 class="team_name">Yating Wang</h3>
−
            <p class="team_designation">MSc Drug Discovery & Translational Biology</p>
+
<p class="team_designation">MSc Drug Discovery & Translational Biology</p>
−
            <p class="team_text">Engineering the lysogenic phage P1 with a CRISPR system to target resistance gene fragments.</p>
+
<p class="team_text">Engineering the lysogenic phage P1 with a CRISPR system to target resistance gene fragments.</p>
−
            <p class="social-icons">
+
<p class="social-icons">
−
              <a href="https://www.facebook.com/profile.php?id=100010921308790" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
+
<a href="https://www.facebook.com/profile.php?id=100010921308790" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
−
            </p>
+
</p>
−
          </div>
+
</div>
−
        </div>
+
</div>
−
      </div>
+
</div>
−
<br>
+
<br>
−
      <div class="row">
+
<div class="row">
−
        <div class="col-md-3 col-md-offset-5">
+
<div class="col-md-3 col-md-offset-5">
−
          <div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
+
<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
−
            <div class="team-img">
+
<div class="team-img">
−
              <img src="https://static.igem.org/mediawiki/2017/1/1b/T--Edinburgh_OG--owen.jpg" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/1/1b/T--Edinburgh_OG--owen.jpg" class="team-pic" alt="">
−
            </div>
+
</div>
−
            <h3 class="team_name">Owen Yeung</h3>
+
<h3 class="team_name">Owen Yeung</h3>
−
            <p class="team_designation">MSc Synthetic Biology & Biotechnology</p>
+
<p class="team_designation">MSc Synthetic Biology & Biotechnology</p>
−
            <p class="team_text">Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.</p>
+
<p class="team_text">Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.</p>
−
            <p class="social-icons">
+
<p class="social-icons">
−
            </p>
+
</p>
−
          </div>
+
</div>
−
        </div>
+
</div>
−
      </div>
+
</div>
−
    </div>
+
</div>
−
<p>The Edinburgh_OG team members developed the iGEM project as part of our <strong>Masters dissertations</strong> at the University of Edinburgh. Due to academic requirements, the iGEM project, PhagED, was divided into individual sub-projects to allow the production of unique dissertations. Each of us completed a dissertation based on the work we did for iGEM, and these dissertations are available upon request.</p>
+
<div class="row" style="margin-top: 0px;">
 +
<p>The Edinburgh_OG team members developed the iGEM project as part of our <strong>Masters dissertations</strong> at the University of Edinburgh. Due to academic requirements, the iGEM project, PhagED, was divided into individual sub-projects to allow
 +
the production of unique dissertations. Each of us completed a dissertation based on the work we did for iGEM, and these dissertations are available upon request.</p>
 +
</div>
  
−
    <div class="row" style="margin-top: 40px;">
+
<div class="row" style="margin-top: 40px;">
−
      <h2 class="subtitle text-center">Supervisors</h2>
+
<h2 class="subtitle text-center">Supervisors</h2>
−
      <div class="col-md-3">
+
<div class="col-md-3">
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        <div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
+
<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
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          <div class="team-img">
+
<div class="team-img">
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            <img src="https://static.igem.org/mediawiki/2017/f/f0/T--Edinburgh_OG--elise.jpg" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/f/f0/T--Edinburgh_OG--elise.jpg" class="team-pic" alt="">
−
          </div>
+
</div>
−
          <h3 class="team_name">Dr Elise Cachat</h3>
+
<h3 class="team_name">Dr Elise Cachat</h3>
−
          <p class="team_designation">School of Biological Sciences</p>
+
<p class="team_designation">School of Biological Sciences</p>
−
          <p class="team_text">Academic supervisor.</p>
+
<p class="team_text">Academic supervisor.</p>
−
          <p class="social-icons">
+
<p class="social-icons">
−
          </p>
+
</p>
−
        </div>
+
</div>
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      </div>
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</div>
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      <div class="col-md-3">
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        <div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
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<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
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          <div class="team-img">
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<div class="team-img">
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            <img src="https://static.igem.org/mediawiki/2017/6/69/T--Edinburgh_OG--heather.jpg" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/6/69/T--Edinburgh_OG--heather.jpg" class="team-pic" alt="">
−
          </div>
+
</div>
−
          <h3 class="team_name">Dr Heather Barker</h3>
+
<h3 class="team_name">Dr Heather Barker</h3>
−
          <p class="team_designation">School of Biological Sciences</p>
+
<p class="team_designation">School of Biological Sciences</p>
−
          <p class="team_text">Supervising lab work.</p>
+
<p class="team_text">Supervising lab work.</p>
−
          <p class="social-icons">
+
<p class="social-icons">
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          </p>
+
</p>
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        </div>
+
</div>
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+
</div>
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      <div class="col-md-3">
+
<div class="col-md-3">
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        <div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
+
<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
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          <div class="team-img">
+
<div class="team-img">
−
            <img src="https://static.igem.org/mediawiki/2017/6/69/T--Edinburgh_OG--holly.jpg" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/6/69/T--Edinburgh_OG--holly.jpg" class="team-pic" alt="">
−
          </div>
+
</div>
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          <h3 class="team_name">Holly Robertson-Dick</h3>
+
<h3 class="team_name">Holly Robertson-Dick</h3>
−
          <p class="team_designation">Industrial Liaison</p>
+
<p class="team_designation">Industrial Liaison</p>
−
          <p class="team_text">Supervising iGEM administrative work.</p>
+
<p class="team_text">Supervising iGEM administrative work.</p>
−
          <p class="social-icons">
+
<p class="social-icons">
−
          </p>
+
</p>
−
        </div>
+
</div>
−
      </div>
+
</div>
−
      <div class="col-md-3">
+
<div class="col-md-3">
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        <div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
+
<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
−
          <div class="team-img">
+
<div class="team-img">
−
            <img src="https://static.igem.org/mediawiki/2017/a/af/T--Edinburgh_OG--john.jpg" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/a/af/T--Edinburgh_OG--john.jpg" class="team-pic" alt="">
−
          </div>
+
</div>
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          <h3 class="team_name">Dr John White</h3>
+
<h3 class="team_name">Dr John White</h3>
−
          <p class="team_designation">School of Chemistry</p>
+
<p class="team_designation">School of Chemistry</p>
−
          <p class="team_text">Supervising phage work.</p>
+
<p class="team_text">Supervising phage work.</p>
−
          <p class="social-icons">
+
<p class="social-icons">
−
          </p>
+
</p>
−
        </div>
+
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+
</div>
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+
</div>
  
−
        <div class="row">
+
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<div class="col-md-3">
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+
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            <div class="team-img">
+
<div class="team-img">
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              <img src="https://static.igem.org/mediawiki/2017/f/fc/T--Edinburgh_OG--filippom.jpg" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/f/fc/T--Edinburgh_OG--filippom.jpg" class="team-pic" alt="">
−
            </div>
+
</div>
−
            <h3 class="team_name">Dr Filippo Menolascina</h3>
+
<h3 class="team_name">Dr Filippo Menolascina</h3>
−
            <p class="team_designation">School of Bioengineering</p>
+
<p class="team_designation">School of Bioengineering</p>
−
            <p class="team_text">Supervising bacteria-phage modelling.</p>
+
<p class="team_text">Supervising bacteria-phage modelling.</p>
−
            <p class="social-icons">
+
<p class="social-icons">
−
            </p>
+
</p>
−
          </div>
+
</div>
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        </div>
+
</div>
−
        <div class="col-md-3">
+
<div class="col-md-3">
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          <div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
+
<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
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            <div class="team-img">
+
<div class="team-img">
−
              <img src="https://static.igem.org/mediawiki/2017/4/4c/T--Edinburgh_OG--russel.jpg" class="team-pic" alt="">
+
<img src="https://static.igem.org/mediawiki/2017/4/4c/T--Edinburgh_OG--russel.jpg" class="team-pic" alt="">
−
            </div>
+
</div>
−
            <h3 class="team_name">Dr Russell Brown</h3>
+
<h3 class="team_name">Dr Russell Brown</h3>
−
            <p class="team_designation">School of Biological Sciences</p>
+
<p class="team_designation">School of Biological Sciences</p>
−
            <p class="team_text">Supervising P1 phage construction.</p>
+
<p class="team_text">Supervising P1 phage construction.</p>
−
            <p class="social-icons">
+
<p class="social-icons">
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            </p>
+
</p>
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          </div>
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    </div>
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</section>
 
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+
<h2 class="subtitle text-center wow fadeInDown animated" data-wow-duration="500ms" data-wow-delay=".3s" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.3s; animation-name: fadeInDown;">Sponsors</h2>
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        <p class="text-center subtitle-des wow fadeInDown animated" data-wow-duration="500ms" data-wow-delay=".5s" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.5s; animation-name: fadeInDown; text-align: center !important;">We are very thankful to those who are helping us to make it happen.</p>
+
<p class="text-center subtitle-des wow fadeInDown animated" data-wow-duration="500ms" data-wow-delay=".5s" style="visibility: visible; animation-duration: 500ms; animation-delay: 0.5s; animation-name: fadeInDown; text-align: center !important;">We are very thankful to those who are helping us to make it happen.</p>
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        <div id="clients-logo" class="owl-carousel">
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<div id="clients-logo" class="owl-carousel">
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<div>
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<img src="https://static.igem.org/mediawiki/2017/f/f7/T--Edinburgh_OG--idt.PNG" alt="">
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<div>
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                                <img src="https://static.igem.org/mediawiki/2017/5/5c/T--Edinburgh_OG--edigenomics.PNG" alt="">
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<img src="https://static.igem.org/mediawiki/2017/5/5c/T--Edinburgh_OG--edigenomics.PNG" alt="">
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                            </div>
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                                <img src="https://static.igem.org/mediawiki/2017/e/e3/T--Edinburgh_OG--eppendorf.PNG" alt="">
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<img src="https://static.igem.org/mediawiki/2017/e/e3/T--Edinburgh_OG--eppendorf.PNG" alt="">
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                            </div>
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                        </div>
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</section>
 
</section>
  
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             Become a sponsor Section Start
 
             Become a sponsor Section Start
 
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+
<section id="call-to-action" style="display:none;">
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<div class="container ">
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<div class="row ">
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<div class="col-md-12 ">
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+
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                      <h2 class="title wow fadeInDown" data-wow-delay=".3s" data-wow-duration="500ms">DO YOU WANT TO HELP US TURN IT INTO REALITY?</h2>
+
<h2 class="title wow fadeInDown" data-wow-delay=".3s" data-wow-duration="500ms">DO YOU WANT TO HELP US TURN IT INTO REALITY?</h2>
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                      <p class="wow fadeInDown" data-wow-delay=".5s" data-wow-duration="500ms">We will be more than happy to accept any kind of support from you either in cash or as free/discounted services or consumables.</p>
+
<p class="wow fadeInDown" data-wow-delay=".5s" data-wow-duration="500ms">We will be more than happy to accept any kind of support from you either in cash or as free/discounted services or consumables.</p>
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</div>
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                <span class="currency">£</span>
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<span class="currency">£</span>
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                <span class="value">500</span>
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<span class="value">500</span>
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Revision as of 01:43, 2 November 2017

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

Antibiotic revolution: a molecular toolkit to re-sensitise
Methycillin resistant S.aureus (MRSA) Vancomycin resistant S.aureus (VRSA) Klebsiella pneumoniae Pseudomonas aeruginosa Enterococcus faecalis/faecium Acinetobacter baumannii

Modular molecular toolkit for re-sensitisation of antibiotic-resistant pathogens using CRISPR delivered by a two-phage system.

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ABOUT PhagED

The threat posed by antibiotic resistant bacteria is a pressing issue which must be addressed. It is difficult and expensive to develop new antibiotics, so our project is designed to make currently available antibiotics useful again. Our aim is to create a toolkit to re-sensitise pathogens to antibiotics using CRISPR and a two-phage system, based on work by Yosef et al. (2015, doi:10.1073/pnas.1500107112). An engineered lysogenic phage will transfer a CRISPR system to its host bacterium, designed to cleave resistance genes and also confer protection from an engineered lytic phage. When this lytic phage is added to the population, it kills any bacteria that have not been successfully re-sensitised. We chose to target genes found in the highly-resistant ESKAPE pathogens, and worked with 4 different phages - P1, lambda, T4 and T7. Our system was modelled in silico and tested empirically on a specially designed E. coli testing platform.

Figure: Yosef et al., 2015

TAKE A CLOSER LOOK

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Project

Get to know PhagED better.

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Parts

Blocks of DNA that we've used.

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Safety

Safety always comes first.

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Human Practices

How does PhagED fit into the context of our lives?

Meet The Team

Filippo Abbondanza

MSc Synthetic Biology & Biotechnology

Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.

Erin Corbett

MSc Synthetic Biology & Biotechnology

Engineering E. coli to create our mock pathogen testing platform, and engineering the lytic T7 phage.

Yunqi He

MSc Biochemistry

Engineering the lysogenic P1 phage with a CRISPR system to target resistance gene fragments.

Ti He

MSc Biotechnology

Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.

Lydia Mapstone

MSc Synthetic Biology & Biotechnology

Engineering E. coli to create our mock pathogen testing platform and engineering the lytic T4 phage using BRED.

Yuri Matsueda

MSc Biotechnology

Engineering the lysogenic P1 phage with a CRISPR system to target resistance gene fragments.

Anton Puzorjov

MSc Bioinformatics

Building a model of bacteria-phage interactions in two-step re-sensitisation combining both lysogenic and lytic phages.

Yating Wang

MSc Drug Discovery & Translational Biology

Engineering the lysogenic phage P1 with a CRISPR system to target resistance gene fragments.


Owen Yeung

MSc Synthetic Biology & Biotechnology

Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.

The Edinburgh_OG team members developed the iGEM project as part of our Masters dissertations at the University of Edinburgh. Due to academic requirements, the iGEM project, PhagED, was divided into individual sub-projects to allow the production of unique dissertations. Each of us completed a dissertation based on the work we did for iGEM, and these dissertations are available upon request.

Supervisors

Dr Elise Cachat

School of Biological Sciences

Academic supervisor.

Dr Heather Barker

School of Biological Sciences

Supervising lab work.

Holly Robertson-Dick

Industrial Liaison

Supervising iGEM administrative work.

Dr John White

School of Chemistry

Supervising phage work.

Dr Filippo Menolascina

School of Bioengineering

Supervising bacteria-phage modelling.

Dr Russell Brown

School of Biological Sciences

Supervising P1 phage construction.

Sponsors

We are very thankful to those who are helping us to make it happen.

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