Team:Edinburgh OG/Attributions
Attributions
We are incredibly grateful to everyone who provided us with help and advice during our project. We couldn’t have done it without you - thank you very much!
Original Work
The project was based on work carried out by Ido Yosef, Miriam Manor, Ruth Kiro and Udi Qimron, described in their 2015 paper “Temperate and lytic bacteriophages programmed to sensitise and kill antibiotic-resistant bacteria”.
doi: 10.1073/pnas.1500107112
Team PhagED
Filippo Abbondanza: responsible for engineering the lambda lysogenic phage to contain a CRISPR system to target resistance gene fragments.
Erin Corbett: responsible for engineering E. coli to create the mock pathogen testing platform, and for developing the T7 lytic phage.
Yunqi He: responsible for engineering the P1 lysogenic phage to contain a CRISPR system to target resistance gene fragments.
Ti He: responsible for engineering the lambda lysogenic phage to contain a CRISPR system to target resistance gene fragments..
Lydia Mapstone: responsible for engineering E. coli to create the mock pathogen testing platform, and for engineering the T4 lytic phage using BRED (Bacteriophage Recombineering of Electroporated DNA).
Yuri Matsueda: responsible for engineering the P1 lysogenic phage to contain a CRISPR system to target resistance gene fragments.
Anton Puzorjov: responsible for building a model of bacteria-phage interactions, combining both lytic and lysogenic phages.
Yating Wang: engineering the P1 lysogenic phage to contain a CRISPR system to target resistance gene fragments.
Owen Yeung: responsible for engineering the lambda lysogenic phage to contain a CRISPR system to target resistance gene fragments.
The Edinburgh_OG team members developed the iGEM project as part of our Masters dissertations at the University of Edinburgh. Due to academic requirements, the iGEM project, PhagED, was divided into individual sub-projects to allow the production of unique dissertations. Each of us completed a dissertation based on the work we did for iGEM, and these dissertations are available upon request.
Acknowledgements
Dr. Heather Barker: supervised our lab work and advised us on our experiments and project planning.
Dr. Dominic Berry: advised us on human practices.
Mr. Thomas Brooks: provided advice and assistance for developing the model.
Dr. Russell Brown (Dr. Baojun Wang Laboratory, University of Edinburgh) : provided the P1 phagemid (unpublished data) , and advice on using P1 phages.
Dr. Elise Cachat: supervised our academic work and advised us on project planning and our dissertations.
Dr. Jane Calvert: advised us on human practices.
The Coli Genetic Stock Center: provided us with lambda, T4 and T7 phages.
Mr. Miguel Cueva: provided us with MoClo backbones.
Dr. Elise Darmon: allowed us to use her fluorescence microscope and advised us on how to use it.
Edinburgh Genomics: provided us with free sequencing services.
Edinburgh iGEM Undergraduate team 2017: for the collaborations.
Prof. Alistair Elfick: advised us on our presentation.
Eppendorf: provided us with free filter tips.
Prof. Chris French: advised us on project planning, safety and our academic work, and allowed us to use his lab equipment.
Dr. Louise Horsfall: advised us on our presentation.
IDT: provided us with free DNA GeneBlock constructs.
Dr. Yusuf Ali Lalloo: provided advice on the concept of our project from the point of view of a General Practitioner.
Prof. David Leach: provided us with information about working with phages, and phage behaviour.
The Leach Lab: provided us with E. coli strains and phage strains.
Mr. Andrew McGregor: provided advice on the concept of our project from the point of view of a farmer.
Dr. Filippo Menolascina: supervised the creation of the bacteria-phage model.
Ms. Karine Moore: provided advice on the concept of our project from the point of view of hospital domestic staff.
Newcastle iGEM Team 2017: provided us with advice on our bacteria-phage model.
Dr. Nick Pantidos: advised us on how to use the electroporator.
Ms. Ellie Powell: designed our team logo.
Mr. Tom Prest: drew our cartoon phages and human practices diagrams.
Dr. Beth Reilly: provided advice on the concept of our project from a veterinary point of view.
Ms. Holly Robertson-Dick: supervised our iGEM work and advised us on fundraising and iGEM requirements.
Dr. Hannah Taylor (Dr. El Karoui laboratory, University of Edinburgh) : provided us with T4-gene2 and MG1655:RecD .
Ms. Laura Tuck: provided the initial DVK_AF plasmid.
Dr. Baojun Wang: advised us on project planning, and allowed us to use his lab equipment.
Dr. John White: advised us on working with phages, and allowed us to use his lab for phage work.
Sponsors
We are very thankful to those who are helping us to make it happen.
