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Revision as of 02:28, 2 November 2017

Document

Results


From the previous procedures, we obtained the following results:



Phase 1: The First Approach

Metabolic Pathway


We observed the production of Glucose 6-phosphate, and an increased of the intermediaries affected by FBP/SBPase. Aditionally, all the compounds reach the steady state with concentrations close to reallity, but the timeline should be reviewed. We considered this model correct for a first approach.

Genetic Pathway

The graphic shows the increase of FBP/SBPasa enzyme, which confirm the expected results. If we expand the timeline, we could observed the steady state.



Metabolic Pathway, B12 Absent

Metabolic Pathway, B12 Present


We observed that in absent of B12 vitamin, the steady state is reached after than the case of presence. Besides, in the first case there is an inicial acumulation of UDP-Glucose, which in the second case is null due to the full production of starch.

Genetic Pathway, B12 Absent

Genetic Pathway, B12 Present

We observed the same behavior of STA1 and STA6 for both cases, therefore insensitive to B12 vitamin. For GBS2 we observed the expected behavior, having a null production of protein, due to the production of antisense RNA, which is confirmed with the formation of double strand DNA.

Phase 2: Sensitivity Analysis

G6P Production from Calvin Cycle

Starch Production from Antisense

G6P Production from Calvin Cycle

Starch Production from Antisense



G6P Production from Calvin Cycle

Starch Production from Antisense



GBS2 effect on Starch Production

STA1 effect on Starch Production

STA6 effect on Starch Production