Team:UChile OpenBio-CeBiB
UChile_OpenBio-CeBiB
Greenhardtii Project
Have you ever looked up at the sky and feel upset about its grayish color? Even in summer's brightest days you can see a lot of smog all around the city, not to mention the shocking view from our highest hills that give the sensation of being embraced by an everlasting cloud of uncertainty about our future. When will it end? We don´t know. How can we avoid this? To that we all have an answer, but constant industrial recklessness makes it pretty difficult to actually change anything. What can we do to help? Well, that's what Greenhardtii Project is about: we want to reduce carbon dioxide emissions by the means of the ability of microalgae to capture this problematic gas and use it to produce materials of interest. To make it even better, we will artificially increase its carbon uptake capacity and hinder the natural synthesis of starch to concentrate glucose availability.
Overview
In regard to the increase in carbon uptake capacity, we focus in the Calvin cycle and consider the possibility of an additional enzyme to accelerate the process. Specifically, we evaluated the expected effects of incorporating FBP/SBPase to this metabolic route. Moreover, we ran short simulations on the kinetics of each reaction involved in the cycle and the consequences of modifying the parameters of the enzyme involved in them. We then came to the conclusion that adding FBP/SBPase is feasible. To allow a constant genetic expression of the FBP/SBPase gene we decided to use a strongly constitutive (but enhanceable with light and temperature) fusion promoter HSP70A/RBCS2, along with the NOSt terminator and a chloroplast peptide signal.
To hinder the synthesis route of starch, we plan to control the deactivation of [[insertar imagen del circuito]]
Lab Procedures and Experimental Design
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Mathematical Modelling
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Human Practices
Strategy
Investigate how much does Chilean people know about contamination, science, biotechnology, eco-friendly production, public spaces, etc. This info will be obtained through several surveys conducted in Santiago de Chile (capital). In response to the information obtained we will start to empower the people by doing workshops, focus groups, talks, visits to laboratories, etc. and design interesting proposal for them. As a general result of all the surveys, we want to model a couple of bioreactors with friendly shapes for people in order to be emplaced in public spaces such as squares, streets, parks, museums, highway, etc.
Financial Resources
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Team Background
[[descripción de nosotros]]
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