The NJU_CHINA team helped us to detect the expression level of Myc-CAR-CD20 and IL-17RA-SynNotch with Western blot. Flag-FOXP3-Jurkat cells expressed the proteins after transfection by electroporation. The result is shown in the figure. They also provided us with human IL-17RA antibodies.
According to the figure, we confirmed that the electroporation was successful and thus we were able to continue our experiments. We would like to thank the NJU-CHINA team for the great help for our experiments and for the provision of IL-17RA antibodies.
