Difference between revisions of "Team:Edinburgh OG/Attributions"

Line 19: Line 19:
 
<div class="column half_size">
 
<div class="column half_size">
 
<h5> Why is this page needed? </h5>
 
<h5> Why is this page needed? </h5>
−
<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
+
<p></p>
 
<p>
 
<p>
−
For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
+
We are incredibly grateful to everyone who provided us with help and advice during our project. We couldn’t have done it without your help.Thank you very much.
 +
 
 +
Original Work
 +
 
 +
The project was based on work carried out by Ido Yosef, Miriam Manor, Ruth Kiro and Udi Qimron, described in their 2015 paper “Temperate and lytic bacteriophages programmed to sensitise and kill antibiotic-resistant bacteria”.
 +
doi: 10.1073/pnas.1500107112
 +
 
 +
Team PhagED
 +
 
 +
Filippo Abbondanza: responsible for engineering the lambda lysogenic phage to contain CRISPR Cpf1 system.
 +
 
 +
Erin Corbett: responsible for engineering E. coli to create the mock pathogen testing platform, and for developing the T7 lytic phage.
 +
 
 +
Yunqi He: responsible for engineering the P1 lysogenic phage to contain CRISPR SpCas9 system.
 +
 +
Ti He: responsible for engineering the lambda lysogenic phage to contain CRISPR SaCas9 system.
 +
 
 +
Lydia Mapstone: responsible for engineering E. coli to create the mock pathogen testing platform, and for engineering the T4 lytic phage using BRED (Bacteriophage Recombineering of Electroporated DNA).
 +
 
 +
Yuri Matsueda: responsible for engineering the P1 lysogenic phage to contain CRISPR SaCas9 system.
 +
 
 +
Anton Puzorjov: responsible for building a model of bacteria-phage interactions, combining both lytic and lysogenic phages.
 +
 
 +
Yating Wang: responsible for engineering the P1 lysogenic phage to contain CRISPR  Cpf1 system.
 +
 
 +
Owen Yeung: responsible for engineering the lambda lysogenic phage to contain  CRISPR.
 +
 
 +
 
 +
Acknowledgements
 +
 
 +
Dr. Heather Barker: supervised our lab work and advised us on our experiments and project planning.
 +
 
 +
Dr. Dominic Berry: advised us on human practices.
 +
 
 +
Dr. Russell Brown: advised us on using P1 phages.
 +
 
 +
Dr. Elise Cachat: supervised our academic work and advised us on project planning and our dissertations.
 +
 
 +
Dr. Jane Calvert: advised us on human practices.
 +
 
 +
The Coli Genetic Stock Center: provided us with lambda, T4 and T7 phages.
 +
 
 +
Dr. Miguel Cueva: provided us with MoClo backbones.
 +
 
 +
Dr. Elise Darmon: allowed us to use her fluorescence microscope and advised us on  how to use it.
 +
 
 +
Edinburgh Genomics: provided us with free sequencing services.
 +
 
 +
Eppendorf: provided us with free filter tips.
 +
 
 +
Dr. Chris French: advised us on project planning, safety and our academic work, and allowed us to use his lab equipment.
 +
 
 +
IDT: provided us with free DNA GeneBlock constructs.
 +
 
 +
Dr. David Leach: provided us with information about working with phages, and phage behaviour.
 +
 
 +
The Leach Lab: provided us with E. coli strains and phage strains.
 +
 
 +
Dr. Filippo Menolascina: supervised the creation of the bacteria-phage model.
 +
 
 +
Mr. Nick Pantidos: advised us on using the electroporator.
 +
 
 +
MS. Ellie Powell: designed our team logo.
 +
 
 +
Ms. Holly Robertson-Dick: supervised our iGEM work and advised us on fundraising and iGEM requirements.
 +
 
 +
Dr. Baojun Wang: advised us on project planning, and allowed us to use his lab equipment.
 +
 
 +
Dr. John White: advised us on working with phages, and allowed us to use his lab for phage work.
 +
 
 +
2017 Edinburgh iGEM Undergraduate team: helped us to do collaborational work.
 +
 
 +
<b>Dr. Beth Reilly<b>: provided advice on concept of our project from veterinary point of view.
 +
 
 +
<b>Mr. Andrew McGregor<b>: provided advice on concept of our project from point of view o farmer.
 +
 
 +
<b>Dr. Yusuf Ali Lalloo<b>: provided advice on concept of our project from point of view of General Practitioner.
 +
 
 +
<b>Ms. Karine Moore<b>: provided advice on concept of our project from point of view of Hospital domestic staff.
 +
 
 +
<b>Mr. Jonathan Lintott<b>: provided advice on concept of our project from industrial point of  view.
 +
 
 +
 
 +
 
 +
 
 +
</p>
 
</div>
 
</div>
  
Line 55: Line 140:
  
 
<div class="column half_size">
 
<div class="column half_size">
−
 
−
<h5>Inspiration</h5>
 
−
<p>Take a look at what other teams have done:</p>
 
−
<ul>
 
−
<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
 
−
<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
 
−
</ul>
 
  
 
</div>
 
</div>
Line 74: Line 150:
 
<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
 
<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
 
<ul>
 
<ul>
−
<li>Does your institution teach an iGEM or synthetic biology course?</li>
+
<li>Does your institution teach an iGEM or synthetic biology course?</li> <p> Yes! We had a MSc course called Synthetic Biology and Biotechnology and four people of the team took that master! <p>
−
<li>When did you start this course?</li>
+
<li>When did you start this course?</li> <p> On september 2017 <p>
−
<li>Are the syllabus and course materials freely available online?</li>
+
<li>Are the syllabus and course materials freely available online?</li> <p> Yes! just google the course and you'll find it!<p>
−
<li>When did you start your brainstorming?</li>
+
<li>When did you start your brainstorming?</li> <p> The brainstorm started in March <p>
−
<li>When did you start in the lab?</li>
+
<li>When did you start in the lab?</li> <p> In the end of may we started the real hands-on work!
−
<li>When did you start working on  your project?</li>
+
<li>When did you start working on  your project?</li> <p> We started to design our project in in the end of April
  
 
</ul>
 
</ul>

Revision as of 20:22, 24 October 2017

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

Attributions

Here is the list of the attributions of the Edinburgh_OG team.

This is a bronze medal requirement. Please see the Medals requirements page for more details.

Why is this page needed?

We are incredibly grateful to everyone who provided us with help and advice during our project. We couldn’t have done it without your help.Thank you very much. Original Work The project was based on work carried out by Ido Yosef, Miriam Manor, Ruth Kiro and Udi Qimron, described in their 2015 paper “Temperate and lytic bacteriophages programmed to sensitise and kill antibiotic-resistant bacteria”. doi: 10.1073/pnas.1500107112 Team PhagED Filippo Abbondanza: responsible for engineering the lambda lysogenic phage to contain CRISPR Cpf1 system. Erin Corbett: responsible for engineering E. coli to create the mock pathogen testing platform, and for developing the T7 lytic phage. Yunqi He: responsible for engineering the P1 lysogenic phage to contain CRISPR SpCas9 system. Ti He: responsible for engineering the lambda lysogenic phage to contain CRISPR SaCas9 system. Lydia Mapstone: responsible for engineering E. coli to create the mock pathogen testing platform, and for engineering the T4 lytic phage using BRED (Bacteriophage Recombineering of Electroporated DNA). Yuri Matsueda: responsible for engineering the P1 lysogenic phage to contain CRISPR SaCas9 system. Anton Puzorjov: responsible for building a model of bacteria-phage interactions, combining both lytic and lysogenic phages. Yating Wang: responsible for engineering the P1 lysogenic phage to contain CRISPR Cpf1 system. Owen Yeung: responsible for engineering the lambda lysogenic phage to contain CRISPR. Acknowledgements Dr. Heather Barker: supervised our lab work and advised us on our experiments and project planning. Dr. Dominic Berry: advised us on human practices. Dr. Russell Brown: advised us on using P1 phages. Dr. Elise Cachat: supervised our academic work and advised us on project planning and our dissertations. Dr. Jane Calvert: advised us on human practices. The Coli Genetic Stock Center: provided us with lambda, T4 and T7 phages. Dr. Miguel Cueva: provided us with MoClo backbones. Dr. Elise Darmon: allowed us to use her fluorescence microscope and advised us on how to use it. Edinburgh Genomics: provided us with free sequencing services. Eppendorf: provided us with free filter tips. Dr. Chris French: advised us on project planning, safety and our academic work, and allowed us to use his lab equipment. IDT: provided us with free DNA GeneBlock constructs. Dr. David Leach: provided us with information about working with phages, and phage behaviour. The Leach Lab: provided us with E. coli strains and phage strains. Dr. Filippo Menolascina: supervised the creation of the bacteria-phage model. Mr. Nick Pantidos: advised us on using the electroporator. MS. Ellie Powell: designed our team logo. Ms. Holly Robertson-Dick: supervised our iGEM work and advised us on fundraising and iGEM requirements. Dr. Baojun Wang: advised us on project planning, and allowed us to use his lab equipment. Dr. John White: advised us on working with phages, and allowed us to use his lab for phage work. 2017 Edinburgh iGEM Undergraduate team: helped us to do collaborational work. Dr. Beth Reilly: provided advice on concept of our project from veterinary point of view. Mr. Andrew McGregor: provided advice on concept of our project from point of view o farmer. Dr. Yusuf Ali Lalloo: provided advice on concept of our project from point of view of General Practitioner. Ms. Karine Moore: provided advice on concept of our project from point of view of Hospital domestic staff. Mr. Jonathan Lintott: provided advice on concept of our project from industrial point of view.

What should this page have?
  • General Support
  • Project support and advice
  • Fundraising help and advice
  • Lab support
  • Difficult technique support
  • Project advisor support
  • Wiki support
  • Presentation coaching
  • Human Practices support
  • Thanks and acknowledgements for all other people involved in helping make a successful iGEM team
Can we base our project on a previous one?

Yes! You can have a project based on a previous team, or based on someone else's idea, as long as you state this fact very clearly and give credit for the original project.

Team training and Project start

Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:

  • Does your institution teach an iGEM or synthetic biology course?

    • Yes! We had a MSc course called Synthetic Biology and Biotechnology and four people of the team took that master!

  • When did you start this course?

    • On september 2017

  • Are the syllabus and course materials freely available online?

    • Yes! just google the course and you'll find it!

  • When did you start your brainstorming?

    • The brainstorm started in March

  • When did you start in the lab?

    • In the end of may we started the real hands-on work!

  • When did you start working on your project?

    • We started to design our project in in the end of April