Difference between revisions of "Team:Edinburgh OG/Experiments"

Line 115: Line 115:
 
           <div class="media-body">
 
           <div class="media-body">
 
             <h4 class="media-heading"><a href="https://static.igem.org/mediawiki/2017/a/aa/T--Edinburgh_OG--titration.pdf">Titration of T4</a></h4>
 
             <h4 class="media-heading"><a href="https://static.igem.org/mediawiki/2017/a/aa/T--Edinburgh_OG--titration.pdf">Titration of T4</a></h4>
 +
          </div>
 +
        </div>
 +
      </div>
 +
 +
      <div class="col-md-4 col-lg-4 col-xs-12">
 +
        <div class="media wow fadeInDown animated" data-wow-duration="500ms" data-wow-delay="1800ms">
 +
          <div class="media-left">
 +
            <div class="icon">
 +
              <i class="ion-ios-flask-outline"></i>
 +
            </div>
 +
          </div>
 +
          <div class="media-body">
 +
            <h4 class="media-heading"><a href="https://static.igem.org/mediawiki/2017/4/47/T--Edinburgh_OG--experiments_fluomicro.pdf">Fluorescence microscopy</a></h4>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="col-md-4 col-lg-4 col-xs-12">
 +
        <div class="media wow fadeInDown animated" data-wow-duration="500ms" data-wow-delay="1800ms">
 +
          <div class="media-left">
 +
            <div class="icon">
 +
              <i class="ion-ios-flask-outline"></i>
 +
            </div>
 +
          </div>
 +
          <div class="media-body">
 +
            <h4 class="media-heading"><a href="https://static.igem.org/mediawiki/2017/d/df/T--Edinburgh_OG--experiments_MoClo.pdf">Molecular Cloning (MoClo)</a></h4>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="col-md-4 col-lg-4 col-xs-12">
 +
        <div class="media wow fadeInDown animated" data-wow-duration="500ms" data-wow-delay="1800ms">
 +
          <div class="media-left">
 +
            <div class="icon">
 +
              <i class="ion-ios-flask-outline"></i>
 +
            </div>
 +
          </div>
 +
          <div class="media-body">
 +
            <h4 class="media-heading"><a href="https://static.igem.org/mediawiki/2017/8/8c/T--Edinburgh_OG--experiments_PCR.pdf">Polymerase chain reaction (PCR)</a></h4>
 +
          </div>
 +
        </div>
 +
      </div>
 +
      <div class="col-md-4 col-lg-4 col-xs-12">
 +
        <div class="media wow fadeInDown animated" data-wow-duration="500ms" data-wow-delay="1800ms">
 +
          <div class="media-left">
 +
            <div class="icon">
 +
              <i class="ion-ios-flask-outline"></i>
 +
            </div>
 +
          </div>
 +
          <div class="media-body">
 +
            <h4 class="media-heading"><a href="https://static.igem.org/mediawiki/2017/e/e0/T--Edinburgh_OG--experiments_T7pCas9.pdf">pCas9 Spacer Cloning</a></h4>
 
           </div>
 
           </div>
 
         </div>
 
         </div>

Revision as of 18:14, 31 October 2017

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

Experiments

Below you can find links to all protocols that we followed to conduct our experiments. If you have any questions please get in touch with us.

Agarose gel

Bacteriophage Recombineering of Electroporated DNA (BRED)

Chemically competent cells CaCl2 method

DNA techniques

Electrocompetent cells

Plasmid transformation into electrocompetent cells

Bacterial glycerol stock

Media

Titration of T4

Fluorescence microscopy

Molecular Cloning (MoClo)

Polymerase chain reaction (PCR)

pCas9 Spacer Cloning