Difference between revisions of "Team:UChile OpenBio-CeBiB/Results"

 
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<h1 style="text-align:center;"> Results </h1>
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<p>Here are the lab results </p>
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<button class="accordion"><p id="panelTitle"><b>First amplification of pBC1. There was no growth of chemicompetent transformants [09/05/2017].</b></p></button>
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<button class="accordion"><p id="panelTitle"><b>pBC1 Amplification. Thermal shock transformation [09/06/2017].</b></p></button>
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<img id="FigA" align="center" src="https://static.igem.org/mediawiki/2017/f/f4/T--UChile_OpenBio-CeBiB--results2.png">
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<button class="accordion"><p id="panelTitle"> <b>Electrophoresis results [10/13/2017]</b></p></button>
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<img id="FigA" align="center" src="https://static.igem.org/mediawiki/2017/2/2b/T--UChile_OpenBio-CeBiB--results5.png ">
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<cite>Gel visualization of amplification of the gblocks Est2 and Est4</cite>
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<img id="FigA" align="center" src="https://static.igem.org/mediawiki/2017/1/12/T--UChile_OpenBio-CeBiB--results6.png ">
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<cite>Gel visualization of the sequences of the second order made to IDT</cite>
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<img id="FigA" align="center" src="https://static.igem.org/mediawiki/2017/8/84/T--UChile_OpenBio-CeBiB--results7.png ">
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<br>
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<cite>Gel extraction for purification of the gBlock of the second order made to IDT</cite>
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<button class="accordion"><p id="panelTitle"><b>Selected colonies for miniprep [10/19/17] </b></p></button>
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<img id="FigA" align="center" src="https://static.igem.org/mediawiki/2017/9/91/T--UChile_OpenBio-CeBiB--results8.png ">
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<cite>LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1-Se 1_2. It grows.</cite>
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<img id="FigA" align="center" src="https://static.igem.org/mediawiki/2017/9/99/T--UChile_OpenBio-CeBiB--results9.png ">
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<cite>LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1-Se 2_2. It grows.</cite>
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<img id="FigA" align="center" src="https://static.igem.org/mediawiki/2017/b/b7/T--UChile_OpenBio-CeBiB--results10.png ">
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<cite>LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBS1-SEQ. It do not grows.</cite>
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<img id="FigA" align="center" src="https://static.igem.org/mediawiki/2017/d/d9/T--UChile_OpenBio-CeBiB--results11.png ">
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<cite>LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3-Est1_2. It grows.</cite>
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<img id="FigA" align="center" src="https://static.igem.org/mediawiki/2017/7/77/T--UChile_OpenBio-CeBiB--results12.png ">
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<cite>LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3-Est2. It grows</cite>
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<img id="FigA" align="center" src=" https://static.igem.org/mediawiki/2017/9/96/T--UChile_OpenBio-CeBiB--results13.png">
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<cite>LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3. It grows</cite>
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<img id="FigA" align="center" src="https://static.igem.org/mediawiki/2017/3/3f/T--UChile_OpenBio-CeBiB--results14.png ">
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<cite>LB and Ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1. It grows</cite>
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<button class="accordion" style="margin-bottom:50px;"><p id="panelTitle"><b> Results of the third restriction test</b></p></button>
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<cite>Superior gel. It is indicated with (N °) the colony from which the plasmid comes. Staircase of 1 kB. Nomenclature: Part-N ° of colony-Plasmid to which the part is bound (Lig plasmid)</cite>
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<cite>Lower gel. It is indicated with (N °) the colony from which the plasmid comes. Staircase of 1 kB. Nomenclature: Part-N ° of colony-Plasmid to which the part is bound (Lig plasmid).</cite>
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Latest revision as of 02:36, 2 November 2017

Document

Results


Here are the lab results










Gel visualization of amplification of the gblocks Est2 and Est4


Gel visualization of the sequences of the second order made to IDT


Gel extraction for purification of the gBlock of the second order made to IDT


LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1-Se 1_2. It grows.


LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1-Se 2_2. It grows.


LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBS1-SEQ. It do not grows.


LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3-Est1_2. It grows.


LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3-Est2. It grows


LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3. It grows


LB and Ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1. It grows


Superior gel. It is indicated with (N °) the colony from which the plasmid comes. Staircase of 1 kB. Nomenclature: Part-N ° of colony-Plasmid to which the part is bound (Lig plasmid)


Lower gel. It is indicated with (N °) the colony from which the plasmid comes. Staircase of 1 kB. Nomenclature: Part-N ° of colony-Plasmid to which the part is bound (Lig plasmid).