Results
Here are the lab results
First amplification of pBC1. There was no growth of chemicompetent transformants [09/05/2017].
[10/13/2017]
Visualized gel amplified of the gblocks Est2 and Est4
Visualized gel of the sequences of the second orden made to IDT
Extraction gel for purification of the gBlock of the second orden made to IDT
Selected colonies for miniprep [10/19/17]
LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1-Se 1_2. It grows.
LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1-Se 2_2. It grows.
LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBS1-SEQ. It do not grows.
LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3-Est1_2. It grows.
LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3-Est2. It grows
LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3. It grows
LB and Ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1. It grows
Results of the third restriction test