Team:UChile OpenBio-CeBiB/Results

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Results


Here are the lab results


  • First amplification of pBC1. There was no growth of chemicompetent transformants [09/05/2017].



  • pBC1 Amplification. Thermal shock transformation [09/06/2017].







  • [10/13/2017]


    • Visualized gel amplified of the gblocks Est2 and Est4


    Visualized gel of the sequences of the second orden made to IDT


    Extraction gel for purification of the gBlock of the second orden made to IDT

  • Selected colonies for miniprep [10/19/17]


    • LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1-Se 1_2. It grows.


    LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1-Se 2_2. It grows.


    LB and ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBS1-SEQ. It do not grows.


    LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3-Est1_2. It grows.


    LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3-Est2. It grows


    LB and Chloranfenicol 50 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pSB1C3. It grows


    LB and Ampicillin 100 ug/ml. BL21DE3 strain of E. coli (No TOP10E) transformed by pBC1. It grows

  • Results of the third restriction test


    • Superior gel. It is indicated with (N °) the colony from which the plasmid comes. Staircase of 1 kB. Nomenclature: Part-N ° of colony-Plasmid to which the part is bound (Lig plasmid)


    Lower gel. It is indicated with (N °) the colony from which the plasmid comes. Staircase of 1 kB. Nomenclature: Part-N ° of colony-Plasmid to which the part is bound (Lig plasmid).