|
|
| Line 22: |
Line 22: |
| | <p class="personal-info">We had multiple evenings together to come up with possible project subjects. We stuck on a DNA detector device using the CRISPR Cas9 system. Later we decided to primarily focus on the detection of bacteriophages in the dairy industry. The day organized by the Rathenau institute helped us to make this decision. </p> | | <p class="personal-info">We had multiple evenings together to come up with possible project subjects. We stuck on a DNA detector device using the CRISPR Cas9 system. Later we decided to primarily focus on the detection of bacteriophages in the dairy industry. The day organized by the Rathenau institute helped us to make this decision. </p> |
| | </div> | | </div> |
| − | </section>
| |
| | | | |
| − | <div class="column half_size">
| + | We had multiple evenings together to come up with possible project subjects. We stuck on a DNA detector device using the CRISPR Cas9 system. Later we decided to primarily focus on the detection of bacteriophages in the dairy industry. The day organized by the Rathenau institute helped us to make this decision. |
| − | <h5> What should this page have?</h5>
| + | |
| | | | |
| − | <ul>
| + | Scientific design |
| − | <li>General Support</li>
| + | - dCas9: Mart |
| − | <li>Project support and advice</li>
| + | - hCas9: Thijs |
| − | <li>Fundraising help and advice</li>
| + | - Reporter plasmid: Sebald and Felix |
| − | <li>Lab support</li>
| + | - Sebald also helped the others with designing their subproject. |
| − | <li>Difficult technique support</li>
| + | - We got tips from our supervisors along the way. |
| − | <li>Project advisor support</li>
| + | |
| − | <li>Wiki support</li>
| + | |
| − | <li>Presentation coaching</li>
| + | |
| − | <li>Human Practices support</li>
| + | |
| − | <li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| | | | |
| | + | Wet Lab |
| | + | Thijs, Felix and Mart worked mostly on their own subprojects. Matthijs and Floor generally assisted the different sub projects. Ada, Meintje, Joana and Sebald helped out a few times when there was demand. |
| | + | Thanks to Jan Kiel and Ger Telkamp for lending us their lab equipment. |
| | + | Thanks to MolGen for providing us with enzymes, chemicals and advice. |
| | + | Thanks to R. Heler for sending us their hCas9 plasmid. |
| | + | Thanks to Chenxi Huang for giving us her dCas9 plasmid. |
| | + | Thanks to Harma Karsens for giving us her pBad plasmid. |
| | + | Thanks to Oscar Kuipers for giving us Nisin and p32 promotor. |
| | | | |
| − | <div class="clear"></div>
| + | Acquisition/Finances |
| | + | Matthijs, Joana and Floor were responsible for getting funds. Matthijs made sure that all the money coming in and out was checked and written down. |
| | + | Matthijs and Floor set up the crowdfunding campaign. |
| | + | Many thanks to Chienes Metus for helping with the inquiries and finance questions. |
| | + | Special thanks to all our generous sponsors and crowdfunding participants. |
| | | | |
| − | <div class="column half_size">
| + | Product Design |
| | + | Ivar and Matthijs worked together to make the bacteriophage detection cartridge. |
| | | | |
| − | <div class="highlight">
| + | Human Practices |
| − | <h5> Can we base our project on a previous one? </h5>
| + | Meintje, Ada, Ivar, Joana and Floor were part of the Human Practices team. They arranged all the interviews and organized events. |
| − | <p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
| + | During phone-and skype calls and visits to factories/places other team members were present as well. |
| − | </div>
| + | Thanks to all the persons who took their time to help us during tours and talks. Sometimes we got new contact from the people we talked to. |
| − | </div>
| + | |
| | | | |
| | + | Modelling |
| | + | The two models were made by Carlos and Angelo. Sebald helped with the scientific input for the models. |
| | | | |
| − | <div class="column half_size">
| + | Education |
| | + | Meintje provided most of the preparations and supervision for the education of high school students and pre-school children. She got help from Joana, Matthijs and Ivar. |
| | + | Ivar did most of the work for creating our ‘Outbreak!’ game. |
| | | | |
| − | <h5>Inspiration</h5>
| + | WIKI construction |
| − | <p>Take a look at what other teams have done:</p>
| + | Ivar and Angelo did most of the code for the WIKI. |
| − | <ul>
| + | Everyone wrote parts for the WIKI. |
| − | <li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
| + | |
| − | <li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
| + | |
| − | <li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
| + | |
| − | <li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
| + | |
| − | </ul>
| + | |
| | | | |
| − | </div>
| + | Visual Design |
| | + | Matthijs made all our posters, wiki images and other designs. |
| | + | Our project video was made by Scripta. |
| | | | |
| − | <div class="clear"></div>
| + | Presentations |
| | + | Felix did gave presentations during most of the events, such as the Dutch iGEM meet-up, the GBB symposium, KIVI event and Giant Jamboree. |
| | + | Mart gave a short presentation during the NBC. |
| | | | |
| − | <div class="column half_size">
| + | Supervisors |
| | + | Thanks to our supervisors Jan Kok, Dirk-Jan Scheffers, Andreas Milias Argeitis, Patricia Alvarez Sieiro, Chenxi Huang, Carsten Pohl and Harma Carsten for helping us troubleshoot tricky parts of our failures. |
| | + | We are especially grateful to Chenxi and Patricia for their enthusiastic input. |
| | | | |
| − | <h5>Team training and Project start</h5>
| + | Others |
| − | <p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
| + | Thanks to Manon Dusseljee for arranging our doorpasses and flight tickets. |
| − | <ul>
| + | Thanks to the friendly reception personnel for always being there for us and helping with organization issues. |
| − | <li>Does your institution teach an iGEM or synthetic biology course?</li>
| + | Thanks to Ivar’s brother for lending us his 3D printer. |
| − | <li>When did you start this course?</li>
| + | Thanks to Meintjes’ personnel pass for free coffee. |
| − | <li>Are the syllabus and course materials freely available online?</li>
| + | |
| − | <li>When did you start your brainstorming?</li>
| + | |
| − | <li>When did you start in the lab?</li>
| + | |
| − | <li>When did you start working on your project?</li>
| + | |
| − | | + | |
| − | </ul>
| + | |
| − | | + | |
| − | </div>
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | </div>
| + | |
| − | </html>
| + | |
Attributions
Project subject
We had multiple evenings together to come up with possible project subjects. We stuck on a DNA detector device using the CRISPR Cas9 system. Later we decided to primarily focus on the detection of bacteriophages in the dairy industry. The day organized by the Rathenau institute helped us to make this decision.
We had multiple evenings together to come up with possible project subjects. We stuck on a DNA detector device using the CRISPR Cas9 system. Later we decided to primarily focus on the detection of bacteriophages in the dairy industry. The day organized by the Rathenau institute helped us to make this decision.
Scientific design
- dCas9: Mart
- hCas9: Thijs
- Reporter plasmid: Sebald and Felix
- Sebald also helped the others with designing their subproject.
- We got tips from our supervisors along the way.
Wet Lab
Thijs, Felix and Mart worked mostly on their own subprojects. Matthijs and Floor generally assisted the different sub projects. Ada, Meintje, Joana and Sebald helped out a few times when there was demand.
Thanks to Jan Kiel and Ger Telkamp for lending us their lab equipment.
Thanks to MolGen for providing us with enzymes, chemicals and advice.
Thanks to R. Heler for sending us their hCas9 plasmid.
Thanks to Chenxi Huang for giving us her dCas9 plasmid.
Thanks to Harma Karsens for giving us her pBad plasmid.
Thanks to Oscar Kuipers for giving us Nisin and p32 promotor.
Acquisition/Finances
Matthijs, Joana and Floor were responsible for getting funds. Matthijs made sure that all the money coming in and out was checked and written down.
Matthijs and Floor set up the crowdfunding campaign.
Many thanks to Chienes Metus for helping with the inquiries and finance questions.
Special thanks to all our generous sponsors and crowdfunding participants.
Product Design
Ivar and Matthijs worked together to make the bacteriophage detection cartridge.
Human Practices
Meintje, Ada, Ivar, Joana and Floor were part of the Human Practices team. They arranged all the interviews and organized events.
During phone-and skype calls and visits to factories/places other team members were present as well.
Thanks to all the persons who took their time to help us during tours and talks. Sometimes we got new contact from the people we talked to.
Modelling
The two models were made by Carlos and Angelo. Sebald helped with the scientific input for the models.
Education
Meintje provided most of the preparations and supervision for the education of high school students and pre-school children. She got help from Joana, Matthijs and Ivar.
Ivar did most of the work for creating our ‘Outbreak!’ game.
WIKI construction
Ivar and Angelo did most of the code for the WIKI.
Everyone wrote parts for the WIKI.
Visual Design
Matthijs made all our posters, wiki images and other designs.
Our project video was made by Scripta.
Presentations
Felix did gave presentations during most of the events, such as the Dutch iGEM meet-up, the GBB symposium, KIVI event and Giant Jamboree.
Mart gave a short presentation during the NBC.
Supervisors
Thanks to our supervisors Jan Kok, Dirk-Jan Scheffers, Andreas Milias Argeitis, Patricia Alvarez Sieiro, Chenxi Huang, Carsten Pohl and Harma Carsten for helping us troubleshoot tricky parts of our failures.
We are especially grateful to Chenxi and Patricia for their enthusiastic input.
Others
Thanks to Manon Dusseljee for arranging our doorpasses and flight tickets.
Thanks to the friendly reception personnel for always being there for us and helping with organization issues.
Thanks to Ivar’s brother for lending us his 3D printer.
Thanks to Meintjes’ personnel pass for free coffee.
