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#content{ | #content{ | ||
width: 100%; | width: 100%; | ||
+ | } | ||
+ | .body-container{ | ||
+ | text-indent:0 !important; | ||
+ | |||
+ | } | ||
+ | h4{line-height:40px; | ||
+ | |||
+ | } | ||
+ | h1,h2,h3,h4{ | ||
+ | font-family: "Century Gothic"; | ||
+ | } | ||
+ | .week{ | ||
+ | font-size:14px !important; | ||
} | } | ||
</style> | </style> | ||
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</div> | </div> | ||
<div class="cd-timeline-content"> | <div class="cd-timeline-content"> | ||
− | <p> | + | <p>Continue to develop Jurkat</p> |
− | <p> | + | <p></p> |
<span class="cd-date">2017/9 / 5-2017 / 9-11</span> | <span class="cd-date">2017/9 / 5-2017 / 9-11</span> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="cd-timeline-content"> | <div class="cd-timeline-content"> | ||
− | <p> | + | <p>Continue to develop Jurkat and HEK293</p> |
− | <p> | + | <p>Transfect plasmids into Jurkat cells by Electrical transfection</p> |
+ | <p>qRCR to verify the mRNA expression of foxp3, Usp7, SynNotch, CAR</p> | ||
<span class="cd-date">2017/9 / 12-2017 / 9/18</span> | <span class="cd-date">2017/9 / 12-2017 / 9/18</span> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="cd-timeline-content"> | <div class="cd-timeline-content"> | ||
− | <p> | + | <p>The cytokine stimulation experiment by IL-6 and IL17A</p> |
+ | <p>Extraction of RNA</p> | ||
+ | <p>Protein extraction</p> | ||
<span class="cd-date">2017/9 / 19-2017 / 9/25</span> | <span class="cd-date">2017/9 / 19-2017 / 9/25</span> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="cd-timeline-content"> | <div class="cd-timeline-content"> | ||
− | <p> | + | <p>Quantify BCA protein</p> |
− | <p> | + | <p>Western blot</p> |
− | + | ||
<span class="cd-date">2017/9 / 26-2017 / 10/2</span> | <span class="cd-date">2017/9 / 26-2017 / 10/2</span> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="cd-timeline-content"> | <div class="cd-timeline-content"> | ||
− | <p>Reverse the RNA into cDNA | + | <p>Reverse the RNA into cDNA</p> |
− | <p> | + | <p>Verify the mRNA expression of Foxp3,Usp7, SynNotch and CAR by qPCR, IP</p> |
<span class="cd-date">2017/10 / 3-2017 / 10/10</span> | <span class="cd-date">2017/10 / 3-2017 / 10/10</span> | ||
</div> | </div> |
Latest revision as of 15:33, 30 October 2017
NOTEBOOK
Gene synthesis at Kingsley Corporation
Order chloramphenicol, related antibodies, BCA kit
2017/6 / 20-2017 / 6/26Gene synthesis at Genescript
Start Interlab
Plasmid transformation, picking a single clone, amplification, determination of absorbance
2017/6 / 27-2017 / 7/3small amount of plasmid was extracted and sequenced
2017/7 / 4-2017 / 7/10Sequence is correct, the plasmid mentioned, get enough plasmid
2017/7 / 11-2017 / 7/17Resuscitation of Jurkat cells
2017/7 / 18-2017 / 7/24Jurkat cell proliferation, partial state is poor, discarded
Recovery another Jurkat cell
Recovery HEK293
2017/7 / 25-2017 / 7/31Continue to develop Jurkat and HEK293
2017/8 / 1-2017 / 8/7Lentivirus plasmid packaging in HEK293, virus particle extraction, concentration
The titer is low and does not meet the requirements
2017/8 / 8-2017 / 8/14Repeated packaging of the virus, transfection of Jurkat cells
Resistance screening
2017/8 / 15-2017 / 8/21RNA and protein extraction
Western blot with the flag antibody, the sample concentration is too large
2017/8 / 22-2017 / 8/28Protein Quantification BCA Kit Quantification of cell extracts and adjustment of sample volume.
Repeat Western blot
Reverse RNA to Cdna
2017/8 / 29-2017 / 9/4Continue to develop Jurkat
2017/9 / 5-2017 / 9-11Continue to develop Jurkat and HEK293
Transfect plasmids into Jurkat cells by Electrical transfection
qRCR to verify the mRNA expression of foxp3, Usp7, SynNotch, CAR
2017/9 / 12-2017 / 9/18The cytokine stimulation experiment by IL-6 and IL17A
Extraction of RNA
Protein extraction
2017/9 / 19-2017 / 9/25Quantify BCA protein
Western blot
2017/9 / 26-2017 / 10/2Reverse the RNA into cDNA
Verify the mRNA expression of Foxp3,Usp7, SynNotch and CAR by qPCR, IP
2017/10 / 3-2017 / 10/10