Difference between revisions of "Team:ECUST/Demonstrate"

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            Demonstrate
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After having designed the project, we both carried out our experiment and manufacture our new photo-bioreactor. We have gained many results through four months hardwork！
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    <h1 id="tables">Key Achivements</h1>
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  <p>1. We successfully constructed the Knock out(BBa_K2308001) and Knock in(BBa_K2308002) plasmids with pDM4 backbone by Gibson assembly.</p><br>
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  <p>2. We successfully knock in the sYFP2(BBa_K2308003) in Rhodobacter Sphaeroides ’s C terminus of H submit of reaction center. Then we did some experiments with strain RC-sYFP2.</p><br>
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  <p>3. We successfully constructed an inducible plasmid pIND4 with IacIq gene from pMCS-eq(BBa_K2308016) which is suitable for Rhodobacter Sphaeroides and characterized it both with optimized sYFP2(BBa_K2308003) and unoptimized sYFP2 (BBa_K864100).</p><br>
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  <p>4. We successfully manufacture a new type of photo-bioreactor and characterized it by some cold mold experiments.</p><br>
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    <h1 id="tables">Results</h1>
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                <div class="col-md-11">1. Electrophoresis patterns of constructed plasmids</div>
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                <img src="https://static.igem.org/mediawiki/2017/7/7c/F1-A.jpeg" height="300px;"><br>
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                <p class="figure"><a href="http://parts.igem.org/Part:BBa_K2308001 ">BBa_K2308001</a></p>
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                <img src="https://static.igem.org/mediawiki/2017/0/04/F1-B.jpg" height="300px;"><br>
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                <p class="figure"><a href="http://parts.igem.org/Part:BBa_K2308002">BBa_K2308002</a></p>
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                <img src="https://static.igem.org/mediawiki/2017/c/c4/F1-C.png" height="300px;"><br>
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                <p class="figure"><a href="http://parts.igem.org/Part:BBa_K2308016">BBa_K2308016</a></p>
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Revision as of 12:35, 31 October 2017






Demonstrate


After having designed the project, we both carried out our experiment and manufacture our new photo-bioreactor. We have gained many results through four months hardwork！

Key Achivements

1. We successfully constructed the Knock out(BBa_K2308001) and Knock in(BBa_K2308002) plasmids with pDM4 backbone by Gibson assembly.


2. We successfully knock in the sYFP2(BBa_K2308003) in Rhodobacter Sphaeroides ’s C terminus of H submit of reaction center. Then we did some experiments with strain RC-sYFP2.


3. We successfully constructed an inducible plasmid pIND4 with IacIq gene from pMCS-eq(BBa_K2308016) which is suitable for Rhodobacter Sphaeroides and characterized it both with optimized sYFP2(BBa_K2308003) and unoptimized sYFP2 (BBa_K864100).


4. We successfully manufacture a new type of photo-bioreactor and characterized it by some cold mold experiments.




Results