Difference between revisions of "Team:Groningen/Results"

Line 75: Line 75:
 
<ul>
 
<ul>
 
<li> <b>Construction</b><br>
 
<li> <b>Construction</b><br>
<img class="imglabh" style="width: 35%; display:inline-block; float:right; margin-bottom:10px;" src="https://static.igem.org/mediawiki/2017/3/39/Gels_081017_collection.svg" alt="hCas9 sub-parts">
+
<img class="imglabh" style="width: 35%; display:inline-block; float:right; margin-bottom:10px;" src="https://static.igem.org/mediawiki/2017/f/f3/Gels_081017_collection2.png" alt="hCas9 sub-parts">
 
For this part we started by.... </li><br>
 
For this part we started by.... </li><br>
 
<li> <b>Considerations for replicating the experiments</b><br>
 
<li> <b>Considerations for replicating the experiments</b><br>

Revision as of 09:47, 1 November 2017


RESULTS

Results

Photography: Joris Bakker

  • In the end we were able to validate three of our parts: dCas9 BBa_K2361000, dCas9VRER BBa_K2361001 and pNisA promoter BBa_K2361009.
  • We submitted sequence verified pUSP45 tracrRNA BBa_K2361003, a CRISPR array BBa_K2361004, a CRISPR array containing a 20 spacer BBa_K2361005, a CRISPR array containing a 21 spacer BBa_K2361006 and a CRISPR array containing empty spacers BBa_K2361007 without furher experimental validation.
  • Unfortunately, sequencing revealed unsuccessful incorporation of the insert during the biobrick construction of two of our parts: p32 BBa_K2361008 and pUsp45 BBa_K2361010.
  • Thijs and Felix in the lab There goes the DNA polymerase So many gels...
    dCas9
    • Construction
      dCas9 colony PCR For this part we started by....

    • Experimental validation
      After creating the part within the pSB1C3 biobrick format,....

    • Considerations for replicating the experiments
      Before this part can be fully integrated into a system, the next parameters should be measured:...

    • Future plans for the project
      Based on these results we suggest that the next experiments can be conducted:...

    dCas9 VRER
    • Construction
      For this part we started by....

    • Experimental validation
      After creating the part within the pSB1C3 biobrick format,....

    • Considerations for replicating the experiments
      Before this part can be fully integrated into a system, the next parameters should be measured:...

    • Future plans for the project
      Based on these results we suggest that the next experiments can be conducted:...

    CRISPR arrays
    • Construction
      For this part we started by....

    • Considerations for replicating the experiments
      Before this part can be fully integrated into a system, the next parameters should be measured:...

    • Future plans for the project
      Based on these results we suggest that the next experiments can be conducted:...

    Lactis toolbox
    • Construction
      For this part we started by....

    • Experimental validation
      After creating the part within the pSB1C3 biobrick format,....

    • Considerations for replicating the experiments
      Before this part can be fully integrated into a system, the next parameters should be measured:...

    • Future plans for the project
      Based on these results we suggest that the next experiments can be conducted:...

    hCas9 operon
    • Construction
      hCas9 sub-parts For this part we started by....

    • Considerations for replicating the experiments
      Before this part can be fully integrated into a system, the next parameters should be measured:...

    • Future plans for the project
      Based on these results we suggest that the next experiments can be conducted:...

    Reporter plasmid
    • Construction
      For this part we started by....

    • Considerations for replicating the experiments
      Before this part can be fully integrated into a system, the next parameters should be measured:...

    • Future plans for the project
      Based on these results we suggest that the next experiments can be conducted:...



    You should also describe what your results mean:
    • Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
    • Show data, but remember all measurement and characterization data must be on part pages in the Registry.
    • Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.
    Project Achievements

    You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

    • A list of linked bullet points of the successful results during your project
    • A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.
    Inspiration

    See how other teams presented their results.

    Next: Notebook