Revision as of 20:32, 24 October 2017

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

Attributions

Here is the list of the attributions of the Edinburgh_OG team.

This is a bronze medal requirement. Please see the Medals requirements page for more details.

Why is this page needed?

We are incredibly grateful to everyone who provided us with help and advice during our project. We couldn’t have done it without your help. Thank you very much. Original Work The project was based on work carried out by Ido Yosef, Miriam Manor, Ruth Kiro and Udi Qimron, described in their 2015 paper “Temperate and lytic bacteriophages programmed to sensitise and kill antibiotic-resistant bacteria”. doi: 10.1073/pnas.1500107112 Team PhagED Filippo Abbondanza: responsible for engineering the lambda lysogenic phage to contain CRISPR Cpf1 system. Erin Corbett: responsible for engineering E. coli to create the mock pathogen testing platform, and for developing the T7 lytic phage. Yunqi He: responsible for engineering the P1 lysogenic phage to contain CRISPR SaCas9 system. Ti He: responsible for engineering the lambda lysogenic phage to contain CRISPR SaCas9 system. Lydia Mapstone: responsible for engineering E. coli to create the mock pathogen testing platform, and for engineering the T4 lytic phage using BRED (Bacteriophage Recombineering of Electroporated DNA). Yuri Matsueda: responsible for engineering the P1 lysogenic phage to contain CRISPR SaCas9 system. Anton Puzorjov: responsible for building a model of bacteria-phage interactions, combining both lytic and lysogenic phages. Yating Wang: responsible for engineering the P1 lysogenic phage to contain CRISPR Cpf1 system. Owen Yeung: responsible for engineering the lambda lysogenic phage to contain CRISPR Cpf1. Acknowledgements Dr. Heather Barker: supervised our lab work and advised us on our experiments and project planning. Dr. Dominic Berry: advised us on human practices. Dr. Russell Brown: advised us on using P1 phages. Dr. Elise Cachat: supervised our academic work and advised us on project planning and our dissertations. Dr. Jane Calvert: advised us on human practices. The Coli Genetic Stock Center: provided us with lambda, T4 and T7 phages. Dr. Miguel Cueva: provided us with MoClo backbones. Dr. Elise Darmon: allowed us to use her fluorescence microscope and advised us on how to use it. Edinburgh Genomics: provided us with free sequencing services. Eppendorf: provided us with free filter tips. Dr. Chris French: advised us on project planning, safety and our academic work, and allowed us to use his lab equipment. IDT: provided us with free DNA GeneBlock constructs. Dr. David Leach: provided us with information about working with phages, and phage behaviour. The Leach Lab: provided us with E. coli strains and phage strains. Dr. Filippo Menolascina: supervised the creation of the bacteria-phage model. Mr. Nick Pantidos: advised us on using the electroporator. MS. Ellie Powell: designed our team logo. Ms. Holly Robertson-Dick: supervised our iGEM work and advised us on fundraising and iGEM requirements. Dr. Baojun Wang: advised us on project planning, and allowed us to use his lab equipment. Dr. John White: advised us on working with phages, and allowed us to use his lab for phage work. 2017 Edinburgh iGEM Undergraduate team: provided us E. coli strain and equipment (see collaborations). Dr. Beth Reilly: provided advice on concept of our project from veterinary point of view. Mr. Andrew McGregor: provided advice on concept of our project from point of view of farmer. Dr. Yusuf Ali Lalloo: provided advice on concept of our project from point of view of General Practitioner. Ms. Karine Moore: provided advice on concept of our project from point of view of Hospital domestic staff. Mr. Jonathan Lintott: provided advice on concept of our project from industrial point of view.

What should this page have?

General Support
Project support and advice
Fundraising help and advice
Lab support
Difficult technique support
Project advisor support
Wiki support
Presentation coaching
Human Practices support
Thanks and acknowledgements for all other people involved in helping make a successful iGEM team

Can we base our project on a previous one?

Yes! You can have a project based on a previous team, or based on someone else's idea, as long as you state this fact very clearly and give credit for the original project.

Team training and Project start

Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:

Does your institution teach an iGEM or synthetic biology course?

Yes! We had a MSc course called Synthetic Biology and Biotechnology and four people of the team took that master!

When did you start this course?

On september 2017

Are the syllabus and course materials freely available online?

Yes! just google the course and you'll find it!

When did you start your brainstorming?

The brainstorm started in March

When did you start in the lab?

In the end of may we started the real hands-on work!

When did you start working on your project?

We started to design our project in in the end of April