Difference between revisions of "Team:ECUST/Demonstrate"
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<p class="figure"><a href="http://parts.igem.org/Part:BBa_K2308016">BBa_K2308016</a></p> | <p class="figure"><a href="http://parts.igem.org/Part:BBa_K2308016">BBa_K2308016</a></p> | ||
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<p class="figure"><a href="http://parts.igem.org/Part:BBa_K2308016">BBa_K2308016</a></p> | <p class="figure"><a href="http://parts.igem.org/Part:BBa_K2308016">BBa_K2308016</a></p> | ||
Revision as of 12:39, 31 October 2017
Key Achivements
1. We successfully constructed the Knock out(BBa_K2308001) and Knock in(BBa_K2308002) plasmids with pDM4 backbone by Gibson assembly.
2. We successfully knock in the sYFP2(BBa_K2308003) in Rhodobacter Sphaeroides ’s C terminus of H submit of reaction center. Then we did some experiments with strain RC-sYFP2.
3. We successfully constructed an inducible plasmid pIND4 with IacIq gene from pMCS-eq(BBa_K2308016) which is suitable for Rhodobacter Sphaeroides and characterized it both with optimized sYFP2(BBa_K2308003) and unoptimized sYFP2 (BBa_K864100).
4. We successfully manufacture a new type of photo-bioreactor and characterized it by some cold mold experiments.
