Difference between revisions of "Team:Groningen/Results"
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<li>We submitted sequence verified pUSP45 tracrRNA <a href="http://parts.igem.org/Part:BBa_K2361003">BBa_K2361003</a>, a CRISPR array <a href="http://parts.igem.org/Part:BBa_K2361004">BBa_K2361004</a>, a CRISPR array containing a 20 spacer <a href="http://parts.igem.org/Part:BBa_K2361005">BBa_K2361005</a>, a CRISPR array containing a 21 spacer <a href="http://parts.igem.org/Part:BBa_K2361006">BBa_K2361006</a> and a CRISPR array containing empty spacers <a href="http://parts.igem.org/Part:BBa_K2361007">BBa_K2361007</a> without furher experimental validation. | <li>We submitted sequence verified pUSP45 tracrRNA <a href="http://parts.igem.org/Part:BBa_K2361003">BBa_K2361003</a>, a CRISPR array <a href="http://parts.igem.org/Part:BBa_K2361004">BBa_K2361004</a>, a CRISPR array containing a 20 spacer <a href="http://parts.igem.org/Part:BBa_K2361005">BBa_K2361005</a>, a CRISPR array containing a 21 spacer <a href="http://parts.igem.org/Part:BBa_K2361006">BBa_K2361006</a> and a CRISPR array containing empty spacers <a href="http://parts.igem.org/Part:BBa_K2361007">BBa_K2361007</a> without furher experimental validation. | ||
<li>Unfortunately, sequencing revealed unsuccessful incorporation of the insert during the biobrick construction of two of our parts: p32 <a href="http://parts.igem.org/Part:BBa_K2361008">BBa_K2361008</a> and pUsp45 <a href="http://parts.igem.org/Part:BBa_K2361010">BBa_K2361010</a>.</li> | <li>Unfortunately, sequencing revealed unsuccessful incorporation of the insert during the biobrick construction of two of our parts: p32 <a href="http://parts.igem.org/Part:BBa_K2361008">BBa_K2361008</a> and pUsp45 <a href="http://parts.igem.org/Part:BBa_K2361010">BBa_K2361010</a>.</li> | ||
| + | <li>Unfortunately, sequencing revealed that the one of the fragments in the CRISPR operon was replaced with something of approximately the same size. Therefore we were not able to submit part <a href="http://parts.igem.org/Part:BBa_K2361002">BBa_K2361002</a>.</li> | ||
</p> | </p> | ||
Revision as of 10:48, 1 November 2017
RESULTS
Results
dCas9
- Construction
For this part we started by.... - Experimental validation
After creating the part within the pSB1C3 biobrick format,.... - Considerations for replicating the experiments
Before this part can be fully integrated into a system, the next parameters should be measured:... - Future plans for the project
Based on these results we suggest that the next experiments can be conducted:...
dCas9 VRER
- Construction
For this part we started by.... - Experimental validation
After creating the part within the pSB1C3 biobrick format,.... - Considerations for replicating the experiments
Before this part can be fully integrated into a system, the next parameters should be measured:... - Future plans for the project
Based on these results we suggest that the next experiments can be conducted:...
CRISPR arrays
- Construction
For this part we started by.... - Considerations for replicating the experiments
Before this part can be fully integrated into a system, the next parameters should be measured:... - Future plans for the project
Based on these results we suggest that the next experiments can be conducted:...
Lactis toolbox
- Construction
For this part we started by.... - Experimental validation
After creating the part within the pSB1C3 biobrick format,.... - Considerations for replicating the experiments
Before this part can be fully integrated into a system, the next parameters should be measured:... - Future plans for the project
Based on these results we suggest that the next experiments can be conducted:...
hCas9 operon
- Construction
For this part we started by.... - Considerations for replicating the experiments
Before this part can be fully integrated into a system, the next parameters should be measured:... - Future plans for the project
Based on these results we suggest that the next experiments can be conducted:...
Reporter plasmid
- Construction
For this part we started by.... - Considerations for replicating the experiments
Before this part can be fully integrated into a system, the next parameters should be measured:... - Future plans for the project
Based on these results we suggest that the next experiments can be conducted:...
You should also describe what your results mean:
- Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
- Show data, but remember all measurement and characterization data must be on part pages in the Registry.
- Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.
Project Achievements
You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.
- A list of linked bullet points of the successful results during your project
- A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.
Inspiration
See how other teams presented their results.
