Difference between revisions of "Team:Groningen/Parts"
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<h1 class="left">Parts</h1> | <h1 class="left">Parts</h1> | ||
| − | <p class="left">Our months of lab work contributed to the construction of several new parts. Please | + | <p class="left">Our months of lab work contributed to the construction of several new parts. Please consult the registry for a more detailed explanation of our parts and the validation work that was performed for it. </p> |
<h5 class="left" >Best Basic Part: dCas9 VRER</h5> | <h5 class="left" >Best Basic Part: dCas9 VRER</h5> | ||
| − | <p class="left"> Our favorite part and the Groningen contender for Best New Basic part is dCas9 VRER. This variation on | + | <p class="left"> Our favorite part and the Groningen contender for Best New Basic part is dCas9 VRER. This variation on our improved dCas9sp for bacterial use, is adapted in such a way that it recognizes different PAM sites. These different sites ensure that the two Cas-complexes of our system do not compete for the binding sites of their counterpart. The difference in PAM sites ensures that the dCas9 VRER protein only binds to the sites which are specifically located on our pre-programmed reporter plasmid. It also prevent it from binding on the injected phage DNA. This also works the other way around as hCas9 only binds on the infecting phage DNA and not on the reporter plasmid, as it would cleave the plasmid upon binding to one of its sites. |
Revision as of 09:45, 28 October 2017
PARTS
Parts
Our months of lab work contributed to the construction of several new parts. Please consult the registry for a more detailed explanation of our parts and the validation work that was performed for it.
Best Basic Part: dCas9 VRER
Our favorite part and the Groningen contender for Best New Basic part is dCas9 VRER. This variation on our improved dCas9sp for bacterial use, is adapted in such a way that it recognizes different PAM sites. These different sites ensure that the two Cas-complexes of our system do not compete for the binding sites of their counterpart. The difference in PAM sites ensures that the dCas9 VRER protein only binds to the sites which are specifically located on our pre-programmed reporter plasmid. It also prevent it from binding on the injected phage DNA. This also works the other way around as hCas9 only binds on the infecting phage DNA and not on the reporter plasmid, as it would cleave the plasmid upon binding to one of its sites.
Parts list
<groupparts>iGEM2017 Groningen</groupparts>
