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<center><img src="https://static.igem.org/mediawiki/2017/5/56/T--CPU_CHINA--des-figure4.png"width = "700"></center> | <center><img src="https://static.igem.org/mediawiki/2017/5/56/T--CPU_CHINA--des-figure4.png"width = "700"></center> | ||
− | + | <h4 align=middle>Figure1. </h4> | |
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<h3 class="ar-title">SynNotch System</h3> | <h3 class="ar-title">SynNotch System</h3> | ||
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<center><img src="https://static.igem.org/mediawiki/2017/5/53/T--CPU_CHINA--eng-figure3_7.png" width = "400"></center> | <center><img src="https://static.igem.org/mediawiki/2017/5/53/T--CPU_CHINA--eng-figure3_7.png" width = "400"></center> | ||
− | + | <h4 align=middle>Figure2. </h4> | |
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<h4><br>In our SynNotch system, we retain the functional sequence of the transmembrane domain and the cleavage site of the Notch 1 protein. At the N-terminus, we fuse the extracellular domain of IL17RA with Notch 1 to specifically recognize IL-17A secreted by Th17 cells, so that our regulatory T cells to obtain the ability to response to IL-17A. We also connect the gene of Gal4-vp64 (a fusion protein) in the downstream of Notch 1. In the presence of inflammatory cytokines IL17A, SynNotch protein is cleaved, and thus Gal4-VP64 fusion protein is detached from the cell membrane. </h4> | <h4><br>In our SynNotch system, we retain the functional sequence of the transmembrane domain and the cleavage site of the Notch 1 protein. At the N-terminus, we fuse the extracellular domain of IL17RA with Notch 1 to specifically recognize IL-17A secreted by Th17 cells, so that our regulatory T cells to obtain the ability to response to IL-17A. We also connect the gene of Gal4-vp64 (a fusion protein) in the downstream of Notch 1. In the presence of inflammatory cytokines IL17A, SynNotch protein is cleaved, and thus Gal4-VP64 fusion protein is detached from the cell membrane. </h4> | ||
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<center><img src="https://static.igem.org/mediawiki/2017/5/5a/T--CPU_CHINA--eng-figure3_9.png" width = "700"></center> | <center><img src="https://static.igem.org/mediawiki/2017/5/5a/T--CPU_CHINA--eng-figure3_9.png" width = "700"></center> | ||
− | + | <h4 align=middle>Figure3. </h4> | |
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<h4><br>At the N terminus, we choose variable region of CD20 monoclonal antibody as the scFv fragment, so that it can accurately identify and bind to B lymphocyte surface antigen CD20. Then, we use two 4-1-BB sequences as a co-stimulatory signal and a CD3Z sequence as a stimulatory signal that allow the signal to be delivered to the cell at a high level, thereby activating Treg cells in the presence of CD20 B lymphocytes. </h4> | <h4><br>At the N terminus, we choose variable region of CD20 monoclonal antibody as the scFv fragment, so that it can accurately identify and bind to B lymphocyte surface antigen CD20. Then, we use two 4-1-BB sequences as a co-stimulatory signal and a CD3Z sequence as a stimulatory signal that allow the signal to be delivered to the cell at a high level, thereby activating Treg cells in the presence of CD20 B lymphocytes. </h4> |
Revision as of 14:50, 30 October 2017