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<h4 align=middle>Figure8. The expression of SynNotch and CAR system in Flag-FOXP3-Jurkat cell line introduced by lentiviral transfection and electroporation</h4> | <h4 align=middle>Figure8. The expression of SynNotch and CAR system in Flag-FOXP3-Jurkat cell line introduced by lentiviral transfection and electroporation</h4> | ||
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− | <h2><br>2.The functioning validation of SynNotch and CAR system</h2> | + | <h2 align=left><br>2.The functioning validation of SynNotch and CAR system</h2> |
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<h4><br>To test SynNotch’s stabilization function on FOXP3 in Treg cells under inflammatory conditions, inflammatory factor IL-6 was added into the culture medium to simulate the microenvironment in RA patients, then western blot and quantitative real-time PCR were performed. Without IL-17A, the expression of FOXP3 was significantly reduced compared to normal one due to the inactivation of SynNotch. However, with the addition of IL-17A, the FOXP3 level was greatly uplifted (Figure 9), indicating that the SynNotch system stabilized FOXP3 in Treg cells with the presence of IL-6.</h4> | <h4><br>To test SynNotch’s stabilization function on FOXP3 in Treg cells under inflammatory conditions, inflammatory factor IL-6 was added into the culture medium to simulate the microenvironment in RA patients, then western blot and quantitative real-time PCR were performed. Without IL-17A, the expression of FOXP3 was significantly reduced compared to normal one due to the inactivation of SynNotch. However, with the addition of IL-17A, the FOXP3 level was greatly uplifted (Figure 9), indicating that the SynNotch system stabilized FOXP3 in Treg cells with the presence of IL-6.</h4> |
Revision as of 02:49, 2 November 2017