Team:ECUST/Demonstrate






Demonstrate


After having designed the project, we both carried out our experiment and manufacture our new photo-bioreactor. We have gained many results through four months hardwork！

Key Achivements

1. We successfully constructed the Knock out(BBa_K2308001) and Knock in(BBa_K2308002) plasmids with pDM4 backbone by Gibson assembly.


2. We successfully knock in the sYFP2(BBa_K2308003) in Rhodobacter Sphaeroides ’s C terminus of H submit of reaction center. Then we did some experiments with strain RC-sYFP2.


3. We successfully constructed an inducible plasmid pIND4 with IacIq gene from pMCS-eq(BBa_K2308016) which is suitable for Rhodobacter Sphaeroides and characterized it both with optimized sYFP2(BBa_K2308003) and unoptimized sYFP2 (BBa_K864100).


4. We successfully manufacture a new type of photo-bioreactor and characterized it by some cold mold experiments.




Results