Team:Groningen/Achievements

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Medal requirements

Bronze

• Register for iGEM, have a great summer and attend the Giant Jamboree.
• Meet all the deliverables on the competition deliverables page.
• Create a page with clear attributions of each aspect of your project.
• Participate in the interlab study (we were the first team who submitted their results! ☺).

Silver

• We validated parts
• We collaborated with lots of other iGEM teams. This was usefull and also great fun, as you can read on the page.
• Human Practices: we collaborated extensively with government institutions and companies to make sure our product is safe and useful.

Gold

• Integrated Human Practices: Through conversations with dairy and fermentation companies we made sure that our product will be as useful as possible.
• The modeling significantly influenced our design, which improved our project.
• We improved one existing biobrick, dCas9 to spdCas9. We improved this part by removing the illegal EcoRI site from the original part, which was not codon optimized. This part was also sequenced verified and compatible with RFC10, 23, 25 and 1000. Moreover this part is experimentally validated by successfully silencing GFP using specific guide RNAs and the protein from this part expressed on a pBAD expression vector. As this part is now biobrick compatible, sequenced verified and experimentally validated makes it an improvement over both BBa K1026001 and BBa_K1994000.

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