Team:ECUST/Lab/Safety
About our Lab
Biosafety Level
1. What is the Safety Level of your lab?
Level 1 (low risk)
2.Which work areas do you use to handle biological materials?
Open bench & Biosafety cabinet
Biosafety Training
1.Have your team members received any safety training yet?
Yes, we have already received safety training.
2. briefly describe the topics that you learned about (or will learn about) in your safety training.
- Lab access and rules (including appropriate clothing, eating and drinking, etc.)
- Responsible individuals (such as lab or departmental specialist or institutional biosafety officer)
- Differences between biosafety levels
- • Biosafety equipment (such as biosafety cabinets)
- Good microbial technique (such as lab practices)
- Disinfection and sterilization
- Emergency procedures
- Transport rules
- • Chemicals, fire and electrical safety
3.Who provided your safety training? Did you get training from multiple people?
Yes, we have already received safety training.
- Departmental specialist
- PIs/instructors
Local rules and regulations
1.Who is responsible for the safety of biology labs at your institution? What are the guidelines for laboratory biosafety? Please give a link to these guidelines, or briefly describe them if you cannot give a link.
Division of laboratory & facility management is responsible for the safety of biology labs in our school.
The above link is the page in which some guidelines can be downloaded. (In Chinese)
2.In your country / region, what are the laws and regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.
About our Project
Project Overview
1.How will your project work?
Our main idea is to expand the absorption spectrum of Rhodobacter sphaeroides by adding fluorescing (such as sYFP2) to the reaction center (C terminal of H subunit) through fusion proteins. Suicide plasmids will be used to knock in the target protein, and plasmids will be conjugated from E.coli to Rhodobacter spheroids. The second part is to produce hydrogen by culturing Rhodobacter spheroids in specific reactor devices.
Future applications
1.How would your project be used in the real world?
- In a factory
- In a small enclosed device
2.What safety or ethical risks would be involved with such a use?
It is very safe and environmentally friendly.
Chassis
1.What is your chassis organism?
E. coli: DH5a, sm10
Others: Rhodobater Sphaeroides
Commends: Rhodobater Sphaeroides is a kind of purple bacteria. Its risk group is 1(low risk) in DSMZ.
Its products, for expamle, Q10 is good to people.
