Team:AshesiGhana/Results

Results

Part Synthesis Results

ACIDITHIOBACILLUS FEROXIDANS DESIGN PARTS

Part Synthesis Status
ORIGIN OF REPLICATION Not Synthesized Successfully
FRET-DONOR Synthesized Successfully
FRET-ACCEPTOR Synthesized Successfully

ECOLI DESIGN PARTS

Part Synthesis Status
FRET-DONOR Synthesized Successfully
FRET-ACCEPTOR Synthesized Successfully
HIGH POTENTIAL IRON SULFUR PROTEIN (HIPIP) Synthesized Successfully
PH RESISTANCE Synthesized Successfully
TETRATHIONATE HYDROLASE Synthesized Successfully
MRUBY 2 Not Synthesized Successfully
NowGFP Not Synthesized Successfully

PARTS DIGESTED AND TRANSFORMED

1. Make up ligation reaction as below
2. High Potential Iron Sulphur Protein (HiPiP)
3. Tetrathionate Hydrolase
4. Fret-Donor
5. Fret-Acceptor

Complete Total Number of Transformations = 5
Successful number of Transformations = 2
Number of failed Transformations = 3

Results pH Resistance Gene

Total number of Transformations = 3
Successful number of Transformations = 2
Number of failed transformations = 1

Total number of gels runs = 3
Successful number of gel runs = 1
Number of failed gel runs = 2

From the gel image in figure 1 above it is evident that the pH resistance gene was inserted in the plasmid, however due to a small concentration of the DNA present, the band of the insert seemed to be blurred out.

From the results obtained and displayed in the graph above, it is evident that the pH resistance gene is effective for pH levels ranging from 5.5 to 7.5, where 7.5 is the pH level of the original media for the bacterial growth. It is also observed that, the resistance gene was not effective with pH levels 4.5 and below, resulting in no bacterial growth over time.

Results FRET Donor

Total number of Transformations = 5
Successful number of Transformations = 2
Number of failed transformations = 3

Total number of gels runs = 3
Successful number of gel runs = 0
Number of failed gel runs = 3

From figure 4 and 3 above, it is observed that the absorption and fluorescence in the sample increased over time as the bacteria growth increased, however the change was not significant and this concludes that the FRET-Donor is not as effective as expected. This could however be attributed to the fact that concentration of the Donor DNA isn’t enough to produce the expected high absorption and fluorescence values.